##gff-version 3 ##sequence-region BBa_K1311007 1 2408 BBa_K1311007 . engineered_region 1548 1559 . + 0 ID=annotation2408485;Name=BBa_B0034 BBa_K1311007 . start_codon 1512 1512 . + 0 ID=annotation2408484;Name=start BBa_K1311007 . engineered_region 1340 1539 . + 0 ID=annotation2408479;Name=BBa_R0010 BBa_K1311007 . engineered_region 1566 2246 . + 0 ID=annotation2408487;Name=BBa_E1010 BBa_K1311007 . polyA_site 2395 2408 . + 0 ID=annotation2408493;Name=polya BBa_K1311007 . non_covalent_binding_site 1428 1465 . + 0 ID=annotation2408480;Name=CAP BBa_K1311007 . engineered_region 135 1169 . + 0 ID=annotation2408472;Name=BBa_C0012 BBa_K1311007 . conserved_region 1552 1555 . + 0 ID=annotation2408486;Name=conserved BBa_K1311007 . polyA_site 1318 1331 . + 0 ID=annotation2408476;Name=polya BBa_K1311007 . stop_codon 2401 2401 . + 0 ID=annotation2408494;Name=stop BBa_K1311007 . stop_codon 1324 1324 . + 0 ID=annotation2408477;Name=stop BBa_K1311007 . sequence_feature 42 128 . + 0 ID=annotation2408471;Name=TALE binding site+RBS BBa_K1311007 . engineered_region 1340 2408 . + 0 ID=annotation2408478;Name=BBa_J04450 BBa_K1311007 . promoter 1500 1505 . + 0 ID=annotation2408482;Name=-10 BBa_K1311007 . non_covalent_binding_site 1505 1539 . + 0 ID=annotation2408483;Name=LacI BBa_K1311007 . engineered_region 1 35 . + 0 ID=annotation2408470;Name=BBa_J23102 BBa_K1311007 . engineered_region 2280 2359 . + 0 ID=annotation2408489;Name=BBa_B0010 BBa_K1311007 . engineered_region 2368 2408 . + 0 ID=annotation2408491;Name=BBa_B0012 BBa_K1311007 . CDS 135 1169 . + 0 ID=annotation2408473;Name=lacI-LVA BBa_K1311007 . CDS 1566 2240 . + 0 ID=annotation2408488;Name=mrfp1 BBa_K1311007 . stem_loop 2375 2394 . + 0 ID=annotation2408492;Name=T7 TE BBa_K1311007 . stem_loop 1214 1257 . + 0 ID=annotation2408475;Name=stem_loop BBa_K1311007 . promoter 1476 1481 . + 0 ID=annotation2408481;Name=-35 BBa_K1311007 . engineered_region 1203 1331 . + 0 ID=annotation2408474;Name=BBa_B0015 BBa_K1311007 . stem_loop 2291 2334 . + 0 ID=annotation2408490;Name=stem_loop >BBa_K1311007 gacagctagctcagtcctaggtactgtgctagctactagagaaagaggggacaaacgtacctcatcaggaacatgttag tgacctcatcaggaacatgttagtgacctcatcaggaacatgttagtactagagatgaaaccagtaacgttatacgatg tcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcg ggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcg ttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccg atcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatct tctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgc actaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggta cgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctc ggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgac tggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgcca acgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtggg atacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaa accagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtga aaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacg acaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct ttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacga caggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggct ttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaaaagaggagaaaatgg cttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcga aatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctg ccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccgg actacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgt tacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggt ccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtg aaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaacc ggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaa cagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgataagtgctagtgtagatcgcccaggcat caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga gtcacactggctcaccttcgggtgggcctttctgcgtttata