BBa_K1312000 1 BBa_K1312000 generate some magnetosome proteins 2014-09-29T11:00:00Z 2015-05-08T01:09:47Z This part come from magnetotactic bacteria AMB-1's genomic sequence. If you use this part, you can generate magnetotactic bacteria AMB-1's magnetosome protein mamL, mamQ, mamB. These proteins are suggested that they are essential to magnetosome formation in AMB-1. We added 6X histidine tag to mamL and mamQ in order to identify these protein by western blotting. MW of mamB and mamQ are similar so we didn't add his tag to mamB. false false _1687_ 0 17653 9 It's complicated false original sequence of mamB has PstI's recognition site so we pointed mutation false Tatsui yusuke annotation2392563 1 mamL range2392563 1 59 313 annotation2390557 1 B0034 range2390557 1 41 52 annotation2390556 1 J23100 range2390556 1 2 36 annotation2413323 1 6Xhistag range2413323 1 1155 1175 annotation2392562 1 mamB range2392562 1 1202 2092 annotation2392564 1 B0034 range2392564 1 1184 1195 annotation2413322 1 6Xhistag range2413322 1 293 313 annotation2392566 1 B0034 range2392566 1 321 332 annotation2392565 1 mamQ range2392565 1 339 1175 BBa_K1312000_sequence 1 gttgacggctagctcagtcctaggtacagtgctagctagaaaagaggagaaaagtggcatggtaagattgatcggatcgttggtgttcggcgggttgatcgtgctgttggcgtcgtccaacgcgcatatggtcgaaacccgcctgggaccactggcgatgattgcgccgcatttcgttgttctgggcataacgttttttctcggtttcgccatcggcatcgtctcggtgctggcgaacgtcatgacgcggcgcaagcagaagtcgccggggaagagcatcgtcatcaagcgccatcatcaccatcaccactgatactagaaaagaggagaaagcggatatggcattaggcgacgcgaatgttggttcggcccctggggtcgacttcagtgcgctgcaacgggtgaagcagtcggaggagctgcttgcccagttgtacgtggtggaggagacgccgcgtcgtttggggcgtgggccggtgcacgctcttatggtcatatcggttttgtcggttgttgcgttcatcgcgacgttgctgatgcgctacaacaccttcgtgaccatgtcggaagacacgcaggcgaagcggtccaattacgaggtgatgatccagcgccgggacaatctgttcggcaatcttgtgaagctgacgctgaaccatgctgcattggagcactcgatcttctcgcatacctcggacaagcggaccgagggcgttgaggctggcaagggcggccctatcggctcggcgctggaacagctgatgaagcagggcgggatcggcaagcttctgggtgatattgggggcggcaaggcgctgctgggggccgacggaggcttcggcaatgcgctgggccgtttgatggcggtggtggagcagtatccgaccatccagtcggtggacacctacaagcatatgatgacgtcgctggtggagatggaagaccgcatcgcgacgcgtcgggaggattacaacgccgccgcctcgacctacaacatcgagatcaccaagtggccgtggaactatctggcgttcatcaccgggttcaagcgggcggagtatttccaggaaaagccggcgggcgacacgccgatcatcacgccgcagctgttccaggaactgttgcctctcaaccatgcgcaggacatcaagaaacatcatcaccatcaccactgatactagagaaagaggagaaagaaccgatgaagttcgaaaattgcagggattgccgcgaggaagtggtgtggtgggcctttaccgccgacatctgcatgaccctgttcaagggggtcctcgggctgatgagcggcagcgtggcgctggtggcggattcgttgcattcgggggcggacgtggtggccagcggggtgacccagctcagcttgaagatttcgaacaagccggcggatgagcgctatccgttcggctacggcaacatccaatacatctcgtcgtccatcgtgggctccttgctgctgatcggggccagcttcttgatgtacggctcggtgatgaagctgatctcggggacctacgaggcgccaagcatcttcgcggcggttggggcgtcggtgacggtgatcgtcaacgagctgatgtatcgctaccagatctgcgtgggcaacgaaaacaacagcccggccatcatcgccaatgcctgggacaaccggtccgacgccatctcgtcggcggcggtgatggtcggcgtgatcgcctcggtgatcggctttcccatcgccgacaccatcgcggccatcggcgtctcggccctggtgggacgcatcggcctggaactgatcggaacctcgatccacggcctgatggacagctcggtggatacggaattgctgcaaaccgcctggcaggtggccatggacacgcccatggtccacagcatctatttcctgcgcggtcgccatgtgggcgaggacgtgcagttcgacatccgcctgcgggtcgatcccaatctgcgcatcaaggacagttccatggtggccgaggcggtacgccggcgcatccaggaggaaatcccccacgcccgcgacatccgcctgttcgtcagcccggcacccgccgccgcggcacgggcctgagcgagagaggggcggcgtcatggacatccggccagagcgtatgttgagcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z