LOCUS       BBa_K1312004            2091 bp    DNA     linear       17-Dec-2024
DEFINITION  pSB1C3 plus 6x His tag
ACCESSION   BBa_K1312004
VERSION     BBa_K1312004.1  
FEATURES             Location/Qualifiers
     misc_feature    22..39
                     /label=6xHistag
     misc_feature    40..42
                     /label=stop codon
     misc_feature    1..21
                     /label=Biobrick suffix
     stem_loop       43..114
                     /label=E. coli his operon terminator
     stem_loop       51..85
                     /label=Stem loop
     primer_bind     155..174
                     /label=Verification reverse (VR) primer binding site
     misc_feature    282..896
                     /label=rep (pMB1)
     misc_feature    297..297
                     /label=ORI
     stem_loop       1063..1168
                     /label=T0 terminator
     CDS             1181..1840
                     /label=Chloramphenicol resistance
     primer_bind     1954..1973
                     /label=Verification forward (VF2) primer binding site
     stem_loop       2030..2033
                     /label=end of terminator
     stem_loop       2053..2062
                     /label=Stem loop
     misc_feature    2072..2091
                     /label=BioBrick prefix
ORIGIN
        1 tactagtagc ggccgctgca gcatcatcac catcaccact aatccggcaa aaaagggcaa
       61 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc
      121 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      181 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      241 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      301 caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      361 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      421 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      481 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      541 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      601 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      661 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      721 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      781 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      841 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      901 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      961 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1021 ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa
     1081 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1141 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1201 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1261 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1321 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1381 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     1441 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1501 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1561 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1621 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1681 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1741 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     1801 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     1861 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1921 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt
     1981 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     2041 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga g
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