BBa_K1312004 1 BBa_K1312004 pSB1C3 plus 6x His tag 2014-10-03T11:00:00Z 2015-05-08T01:09:47Z pSB1C3 This part is pSB1C3 which introduced 6xHis tag and stop codon UAA after Biobrick Suffix. Insert promoter,RBS and coding protein gene which is from start codon to before stop codon, if you want to add 6xHis tag to protein. false false _1687_ 0 17633 9 Not in stock false We added 6xHistag and stop codon UAA to after Biobrick Suffix. false Yutaka Murata annotation2428839 1 T0 terminator range2428839 1 1063 1168 annotation2428834 1 E. coli his operon terminator range2428834 1 43 114 annotation2428837 1 rep (pMB1) range2428837 1 282 896 annotation2428841 1 Verification forward (VF2) primer binding site range2428841 1 1954 1973 annotation2428842 1 end of terminator range2428842 1 2030 2033 annotation2428835 1 Stem loop range2428835 1 51 85 annotation2428840 1 Chloramphenicol resistance range2428840 1 1181 1840 annotation2428832 1 stop codon range2428832 1 40 42 annotation2428838 1 ORI range2428838 1 297 297 annotation2428843 1 Stem loop range2428843 1 2053 2062 annotation2428831 1 6xHistag range2428831 1 22 39 annotation2428844 1 BioBrick prefix range2428844 1 2072 2091 annotation2428836 1 Verification reverse (VR) primer binding site range2428836 1 155 174 annotation2428833 1 Biobrick suffix range2428833 1 1 21 BBa_K1312004_sequence 1 tactagtagcggccgctgcagcatcatcaccatcaccactaatccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z