BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1313003
1
RBS+OxRed
RBS + Oxoacyl Reductase
2014-10-11T11:00:00Z
2015-05-08T01:09:47Z
Designed composite by ITESM CEM
This composite has an associated RBS (BBa_B0034) to BBa_K1313001.
false
false
_1688_
0
19941
9
It's complicated
false
TACTAG scar
false
Oliva S??nchez
component2417602
1
BBa_B0034
component2417603
1
BBa_K1313001
annotation2417602
1
BBa_B0034
range2417602
1
1
12
annotation2417603
1
BBa_K1313001
range2417603
1
19
960
BBa_K1313001
1
BBa_K1313001
Oxoacyl Reductase
2014-10-10T11:00:00Z
2015-05-08T01:09:47Z
ATGAAACGCCTGGTCTGTGTTCTGCTGGTCTGCTCGTCTGCTGTTGCTCCGCTGCATCATAGCCTGGTTGATCAC
CCGTCTGCCCATCCGGGTCATGGCGGTGGCCCTGTTAGTGCGGGCGTCGCCCTGGTGACCGGTGGCTCCCGTGGC
GTTGGTAAAGGCGTCGCAACGGCACTGGGTGCGGCGGGTTGGACCGTGTATGTTACGGGTCGTGGCCCGATGACC
GAAGATTGCCCGCTGAGTGCATCCGCTCGTACCGTTACGGGTGCGGGTGGCACCGGTATTGCAGTCGCCTGTGAC
CACCGTGAAGATGCAGACGTGCGTGCACTGTTTGCTCGCATCAGTGATGAACAGGCCGGTCGCCTGGACCTGCTG
GTTAACAATGTCTGGGCAGCTCCGAAAGGCTTTGGTGGCTTCAGCACCCCGTTCTGGGAACGTCCGCTGGATGAC
TGGGATTCTCTGATTGGTGTTGGCCTGCGTGCGCATTATGTGGCCTCAGTTGAAGCAGCACGTCTGATGGTGCCG
CGTCGCTCGGGTCTGATTGCGAACATCAGCTCTTTTGGTACCCGCGGCCATCTGCACTCAGTGCTGTACGGCATG
TCGAAAGCAGGCCTGGATAAAATGGCTAGCGACATGGCAGCTGAACTGGCCCCGCACGATGTCCGTGCAGTGAGT
CTGTGGCCGGGCATTGTGCGCACCGAAATGATCGTTGCGTCCGGTCTGGAAACGATCGCCGGCTTTAGCGTTGCA
GATGCTGAATCTCCGGAATTTATTGGTGAAGTCATCTGCGCACTGACCGCCGACCCGACGCTGCCGCAGCGTTCT
GGTCATACCTTCGTCACGGCAGAACTGGCTGTGGAATACGGTATTACCGATACGCAAGGTCGTACCCCGCCGAGC
CACCGTGAACCGTTTGGTGGCGGTCCGCTGTTCAATATCACG
7-ketocholesterol is the result of the oxidation that exists in the bloodstream. 7-keto group is being reduced by Oxoacyl Reductase into 7-OH. The identity of this enzyme has not yet veryfied. This nucleotidic sequence was based on Jacques Mathieu studies.He identified gene expression analysis using microarrays and in silico amino acidic predictions.
false
false
_1688_
0
19941
9
It's complicated
false
Codon optimization for a better expression on E.coli.
false
Eddie Cano
BBa_K1313003_sequence
1
aaagaggagaaatactagatgaaacgcctggtctgtgttctgctggtctgctcgtctgctgttgctccgctgcatcatagcctggttgatcacccgtctgcccatccgggtcatggcggtggccctgttagtgcgggcgtcgccctggtgaccggtggctcccgtggcgttggtaaaggcgtcgcaacggcactgggtgcggcgggttggaccgtgtatgttacgggtcgtggcccgatgaccgaagattgcccgctgagtgcatccgctcgtaccgttacgggtgcgggtggcaccggtattgcagtcgcctgtgaccaccgtgaagatgcagacgtgcgtgcactgtttgctcgcatcagtgatgaacaggccggtcgcctggacctgctggttaacaatgtctgggcagctccgaaaggctttggtggcttcagcaccccgttctgggaacgtccgctggatgactgggattctctgattggtgttggcctgcgtgcgcattatgtggcctcagttgaagcagcacgtctgatggtgccgcgtcgctcgggtctgattgcgaacatcagctcttttggtacccgcggccatctgcactcagtgctgtacggcatgtcgaaagcaggcctggataaaatggctagcgacatggcagctgaactggccccgcacgatgtccgtgcagtgagtctgtggccgggcattgtgcgcaccgaaatgatcgttgcgtccggtctggaaacgatcgccggctttagcgttgcagatgctgaatctccggaatttattggtgaagtcatctgcgcactgaccgccgacccgacgctgccgcagcgttctggtcataccttcgtcacggcagaactggctgtggaatacggtattaccgatacgcaaggtcgtaccccgccgagccaccgtgaaccgtttggtggcggtccgctgttcaatatcacg
BBa_K1313001_sequence
1
atgaaacgcctggtctgtgttctgctggtctgctcgtctgctgttgctccgctgcatcatagcctggttgatcacccgtctgcccatccgggtcatggcggtggccctgttagtgcgggcgtcgccctggtgaccggtggctcccgtggcgttggtaaaggcgtcgcaacggcactgggtgcggcgggttggaccgtgtatgttacgggtcgtggcccgatgaccgaagattgcccgctgagtgcatccgctcgtaccgttacgggtgcgggtggcaccggtattgcagtcgcctgtgaccaccgtgaagatgcagacgtgcgtgcactgtttgctcgcatcagtgatgaacaggccggtcgcctggacctgctggttaacaatgtctgggcagctccgaaaggctttggtggcttcagcaccccgttctgggaacgtccgctggatgactgggattctctgattggtgttggcctgcgtgcgcattatgtggcctcagttgaagcagcacgtctgatggtgccgcgtcgctcgggtctgattgcgaacatcagctcttttggtacccgcggccatctgcactcagtgctgtacggcatgtcgaaagcaggcctggataaaatggctagcgacatggcagctgaactggccccgcacgatgtccgtgcagtgagtctgtggccgggcattgtgcgcaccgaaatgatcgttgcgtccggtctggaaacgatcgccggctttagcgttgcagatgctgaatctccggaatttattggtgaagtcatctgcgcactgaccgccgacccgacgctgccgcagcgttctggtcataccttcgtcacggcagaactggctgtggaatacggtattaccgatacgcaaggtcgtaccccgccgagccaccgtgaaccgtttggtggcggtccgctgttcaatatcacg
BBa_B0034_sequence
1
aaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z