BBa_K1313004
1
NeoR
Neomycin Resistance
2014-10-11T11:00:00Z
2016-01-28T01:43:08Z
This gene was obtained from pCDNA 3.1 myc His(A).
Neomycin resistance gene. This gene can be used for selection of cells with an efficient transformation. This gene codes neomycin phosphotransferase which breaks neomycin, kanamycin, and gentamicin molecules. If you use these gene as selectivity gene in bacteria, and your laboratory lacks neomycin, you can replace it for kanamycin by adding 35 mg/ml per liter of LB media. The best concentration of neomycin to use is 80 mg/ml per liter of LB media. <br><br>
This gene can be also used as a selective gene for mammalian cells using gentamicin.
false
false
_1688_
4206
19941
9
In stock
true
Pst1 site cuts gene.
false
Carlos Meza
BBa_K1313004_sequence
1
atggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctaataat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z