LOCUS BBa_K1314001 1089 bp DNA linear 22-Dec-2024 DEFINITION nifH promoter with mRFP reporter ACCESSION BBa_K1314001 VERSION BBa_K1314001.1 FEATURES Location/Qualifiers promoter 1..220 /label=nifH promoter promoter 1..220 /label=BBa_K1314000 misc_feature 233..236 /label=conserved RBS 229..240 /label=BBa_B0034 CDS 247..921 /label=mrfp1 misc_feature 928..952 /label=Help:Barcodes CDS 247..927 /label=BBa_E1010 misc_feature 229..952 /label=BBa_I13502 stem_loop 972..1015 /label=stem_loop misc_feature 961..1040 /label=BBa_B0010 terminator 961..1040 /label=BBa_B0010 stem_loop 1056..1075 /label=T7 TE misc_feature 1082..1082 /label=stop polyA_site 1076..1089 /label=polya misc_feature 1049..1089 /label=BBa_B0012 terminator 1049..1089 /label=BBa_B0012 terminator 961..1089 /label=BBa_B0015 ORIGIN 1 tgtcagtttt gtcacagggg gccggaccag gatggtggac gctcgatggg gatgtcgggc 61 cattgttcgg ttgtagcaat tacaacagtc ggagtagggg gattgtaggg ggattgttgt 121 gtatcagacc gccctgctgc tcccgtcgat ggataattaa tcatttaaaa tcaatggttt 181 atttatgtgt tgcgggtgct ggcacagacg ctgcattacc tactagagaa agaggagaaa 241 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg 301 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac 361 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg 421 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac 481 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac 541 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc 601 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa 661 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa 721 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa 781 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa 841 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa 901 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag 961 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1021 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1081 gcgtttata //