##gff-version 3
##sequence-region BBa_K1314004 1 1081
BBa_K1314004	.	engineered_region	159	1081	.	+	0	ID=BBa_I13521;Name=BBa_I13521
BBa_K1314004	.	CDS	239	919	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_K1314004	.	engineered_tag	920	944	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1314004	.	CDS	239	913	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1314004	.	terminator	953	1032	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_K1314004	.	engineered_region	953	1032	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1314004	.	stem_loop	964	1007	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1314004	.	ribosome_entry_site	221	232	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_K1314004	.	conserved_region	225	228	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1314004	.	terminator	1041	1081	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_K1314004	.	polyA_site	1068	1081	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1314004	.	engineered_region	1041	1081	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1314004	.	stop_codon	1074	1074	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1314004	.	stem_loop	1048	1067	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1314004	.	promoter	159	212	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_K1314004	.	promoter	178	183	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1314004	.	promoter	201	206	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1314004	.	engineered_region	159	212	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1314004	.	non_covalent_binding_site	159	177	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1314004	.	non_covalent_binding_site	184	202	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1314004	.	sequence_feature	1	150	.	+	0	ID=BBa_K1314002;Name=BBa_K1314002
BBa_K1314004	.	engineered_region	1	150	.	+	0	ID=annotation2397292;Name=random sequence/spacer;Parent=BBa_K1314002
>BBa_K1314004
ggaattggggcccttgtggcctaaatccaaccgcggatcagtcagggcgcggatcccctgcaacgtcgcgacgctagag
atactctatccagcggtagctcgtgtggttcaaagggtaagccgtcacgctggcttcggaggtctatctgctactagag
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgag
ttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtc
cgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtt
accgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccg
acggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaa
aggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaa
aaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcg
ttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata