LOCUS BBa_K1314007 4127 bp DNA linear 05-May-2025 DEFINITION nifH promoter+B0034+T7 RNA polymerase+ B0015+ random sequence+ptet mRFP reporter ACCESSION BBa_K1314007 VERSION BBa_K1314007.1 FEATURES Location/Qualifiers promoter 1..220 /label=nifH promoter promoter 1..220 /label=BBa_K1314000 misc_feature 233..236 /label=conserved RBS 229..240 /label=BBa_B0034 misc_feature 247..249 /label=start misc_feature 2896..2898 /label=stop misc_feature 2899..2901 /label=stop CDS 250..2895 /label=protein CDS 247..2901 /label=BBa_K145001 stem_loop 2921..2964 /label=stem_loop misc_feature 2910..2989 /label=BBa_B0010 terminator 2910..2989 /label=BBa_B0010 stem_loop 3005..3024 /label=T7 TE misc_feature 3031..3031 /label=stop polyA_site 3025..3038 /label=polya misc_feature 2998..3038 /label=BBa_B0012 terminator 2998..3038 /label=BBa_B0012 terminator 2910..3038 /label=BBa_B0015 misc_feature 1..3038 /label=BBa_K1314005 misc_feature 3047..3196 /label=random sequence/spacer misc_feature 3047..3196 /label=BBa_K1314002 misc_feature 3205..3223 /label=TetR 1 misc_feature 3205..3258 /label=BBa_R0040 promoter 3224..3229 /label=-35 misc_feature 3230..3248 /label=TetR 2 promoter 3247..3252 /label=-10 promoter 3205..3258 /label=BBa_R0040 misc_feature 3271..3274 /label=conserved RBS 3267..3278 /label=BBa_B0034 CDS 3285..3959 /label=mrfp1 misc_feature 3966..3990 /label=Help:Barcodes CDS 3285..3965 /label=BBa_E1010 stem_loop 4010..4053 /label=stem_loop misc_feature 3999..4078 /label=BBa_B0010 terminator 3999..4078 /label=BBa_B0010 stem_loop 4094..4113 /label=T7 TE misc_feature 4120..4120 /label=stop polyA_site 4114..4127 /label=polya misc_feature 4087..4127 /label=BBa_B0012 terminator 4087..4127 /label=BBa_B0012 misc_feature 3205..4127 /label=BBa_I13521 misc_feature 3047..4127 /label=BBa_K1314004 ORIGIN 1 tgtcagtttt gtcacagggg gccggaccag gatggtggac gctcgatggg gatgtcgggc 61 cattgttcgg ttgtagcaat tacaacagtc ggagtagggg gattgtaggg ggattgttgt 121 gtatcagacc gccctgctgc tcccgtcgat ggataattaa tcatttaaaa tcaatggttt 181 atttatgtgt tgcgggtgct ggcacagacg ctgcattacc tactagagaa agaggagaaa 241 tactagatga acacgattaa catcgctaag aacgacttct ctgacatcga actggctgct 301 atcccgttca acactctggc tgaccattac ggtgagcgtt tagctcgcga acagttggcc 361 cttgagcatg agtcttacga gatgggtgaa gcacgcttcc gcaagatgtt tgagcgtcaa 421 cttaaagctg gtgaggttgc ggataacgct gccgccaagc ctctcatcac taccctactc 481 cctaagatga ttgcacgcat caacgactgg tttgaggaag tgaaagctaa gcgcggcaag 541 cgcccgacag ccttccagtt cctgcaagaa atcaagccgg aagccgtagc gtacatcacc 601 attaagacca ctctggcttg cctaaccagt gctgacaata caaccgttca ggctgtagca 661 agcgcaatcg gtcgggccat tgaggacgag gctcgcttcg gtcgtatccg tgaccttgaa 721 gctaagcact tcaagaaaaa cgttgaggaa caactcaaca agcgcgtagg gcacgtctac 781 aagaaagcat ttatgcaagt tgtcgaggct gacatgctct ctaagggtct actcggtggc 841 gaggcgtggt cttcgtggca taaggaagac tctattcatg taggagtacg ctgcatcgag 901 atgctcattg agtcaaccgg aatggttagc ttacaccgcc aaaatgctgg cgtagtaggt 961 caagactctg agactatcga actcgcacct gaatacgctg aggctatcgc aacccgtgca 1021 ggtgcgctgg ctggcatctc tccgatgttc caaccttgcg tagttcctcc taagccgtgg 1081 actggcatta ctggtggtgg ctattgggct aacggtcgtc gtcctctggc gctggtgcgt 1141 actcacagta agaaagcact gatgcgctac gaagacgttt acatgcctga ggtgtacaaa 1201 gcgattaaca ttgcgcaaaa caccgcatgg aaaatcaaca agaaagtcct agcggtcgcc 1261 aacgtaatca ccaagtggaa gcattgtccg gtcgaggaca tccctgcgat tgagcgtgaa 1321 gaactcccga tgaaaccgga agacatcgac atgaatcctg aggctctcac cgcgtggaaa 1381 cgtgctgccg ctgctgtgta ccgcaaggac aaggctcgca agtctcgccg tatcagcctt 1441 gagttcatgc ttgagcaagc caataagttt gctaaccata aggccatctg gttcccttac 1501 aacatggact ggcgcggtcg tgtttacgct gtgtcaatgt tcaacccgca aggtaacgat 1561 atgaccaaag gactgcttac gctggcgaaa ggtaaaccaa tcggtaagga aggttactac 1621 tggctgaaaa tccacggtgc aaactgtgcg ggtgtcgata aggttccgtt ccctgagcgc 1681 atcaagttca ttgaggaaaa ccacgagaac atcatggctt gcgctaagtc tccactggag 1741 aacacttggt gggctgagca agattctccg ttctgcttcc ttgcgttctg ctttgagtac 1801 gctggggtac agcaccacgg cctgagctat aactgctccc ttccgctggc gtttgacggg 1861 tcttgctctg gcatccagca cttctccgcg atgctccgag atgaggtagg tggtcgcgcg 1921 gttaacttgc ttcctagtga aaccgttcag gacatctacg ggattgttgc taagaaagtc 1981 aacgagattc tacaagcaga cgcaatcaat gggaccgata acgaagtagt taccgtgacc 2041 gatgagaaca ctggtgaaat ctctgagaaa gtcaagctgg gcactaaggc actggctggt 2101 caatggctgg cttacggtgt tactcgcagt gtgactaagc gttcagtcat gacgctggct 2161 tacgggtcca aagagttcgg cttccgtcaa caagtgctgg aagataccat tcagccagct 2221 attgattccg gcaagggtct gatgttcact cagccgaatc aggctgctgg atacatggct 2281 aagctgattt gggaatctgt gagcgtgacg gtggtagctg cggttgaagc aatgaactgg 2341 cttaagtctg ctgctaagct gctggctgct gaggtcaaag ataagaagac tggagagatt 2401 cttcgcaagc gttgcgctgt gcattgggta actcctgatg gtttccctgt gtggcaggaa 2461 tacaagaagc ctattcagac gcgcttgaac ctgatgttcc tcggtcagtt ccgcttacag 2521 cctaccatta acaccaacaa agatagcgag attgatgcac acaaacagga gtctggtatc 2581 gctcctaact ttgtacacag ccaagacggt agccaccttc gtaagactgt agtgtgggca 2641 cacgagaagt acggaatcga atcttttgca ctgattcacg actccttcgg taccattccg 2701 gctgacgctg cgaacctgtt caaagcagtg cgcgaaacta tggttgacac atatgagtct 2761 tgtgatgtac tggctgattt ctacgaccag ttcgctgacc agttgcacga gtctcaattg 2821 gacaaaatgc cagcacttcc ggctaaaggt aacttgaacc tccgtgacat cttagagtcg 2881 gacttcgcgt tcgcgtaata atactagagc caggcatcaa ataaaacgaa aggctcagtc 2941 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 3001 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagggaa ttggggccct 3061 tgtggcctaa atccaaccgc ggatcagtca gggcgcggat cccctgcaac gtcgcgacgc 3121 tagagatact ctatccagcg gtagctcgtg tggttcaaag ggtaagccgt cacgctggct 3181 tcggaggtct atctgctact agagtcccta tcagtgatag agattgacat ccctatcagt 3241 gatagagata ctgagcacta ctagagaaag aggagaaata ctagatggct tcctccgaag 3301 acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg 3361 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac 3421 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc 3481 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt 3541 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta 3601 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg 3661 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt 3721 ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga 3781 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac 3841 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg 3901 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt 3961 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa 4021 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 4081 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //