##gff-version 3
##sequence-region BBa_K1314008 1 4129
BBa_K1314008	.	engineered_region	1	3040	.	+	0	ID=BBa_K1314006;Name=BBa_K1314006
BBa_K1314008	.	CDS	249	2903	.	+	0	ID=BBa_K145001;Name=BBa_K145001;Parent=BBa_K1314006
BBa_K1314008	.	stop_codon	2898	2900	.	+	0	ID=annotation1968217;Name=stop;Parent=BBa_K145001
BBa_K1314008	.	stop_codon	2901	2903	.	+	0	ID=annotation1968218;Name=stop;Parent=BBa_K145001
BBa_K1314008	.	start_codon	249	251	.	+	0	ID=annotation1968216;Name=start;Parent=BBa_K145001
BBa_K1314008	.	CDS	252	2897	.	+	0	ID=annotation1968243;Name=protein;Parent=BBa_K145001
BBa_K1314008	.	promoter	1	220	.	+	0	ID=BBa_K1314000;Name=BBa_K1314000;Parent=BBa_K1314006
BBa_K1314008	.	promoter	1	220	.	+	0	ID=annotation2396224;Name=nifH promoter;Parent=BBa_K1314000
BBa_K1314008	.	terminator	2912	3040	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1314006
BBa_K1314008	.	terminator	2912	2991	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1314008	.	engineered_region	2912	2991	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1314008	.	stem_loop	2923	2966	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1314008	.	terminator	3000	3040	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1314008	.	polyA_site	3027	3040	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1314008	.	engineered_region	3000	3040	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1314008	.	stop_codon	3033	3033	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1314008	.	stem_loop	3007	3026	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1314008	.	ribosome_entry_site	229	242	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K1314006
BBa_K1314008	.	conserved_region	235	238	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K1314008	.	engineered_region	3049	4129	.	+	0	ID=BBa_K1314004;Name=BBa_K1314004
BBa_K1314008	.	engineered_region	3207	4129	.	+	0	ID=BBa_I13521;Name=BBa_I13521;Parent=BBa_K1314004
BBa_K1314008	.	terminator	4001	4080	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_K1314008	.	engineered_region	4001	4080	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1314008	.	stem_loop	4012	4055	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1314008	.	CDS	3287	3967	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_K1314008	.	engineered_tag	3968	3992	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1314008	.	CDS	3287	3961	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1314008	.	ribosome_entry_site	3269	3280	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_K1314008	.	conserved_region	3273	3276	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1314008	.	terminator	4089	4129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_K1314008	.	polyA_site	4116	4129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1314008	.	engineered_region	4089	4129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1314008	.	stop_codon	4122	4122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1314008	.	stem_loop	4096	4115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1314008	.	promoter	3207	3260	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_K1314008	.	promoter	3226	3231	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1314008	.	engineered_region	3207	3260	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1314008	.	non_covalent_binding_site	3207	3225	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1314008	.	promoter	3249	3254	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1314008	.	non_covalent_binding_site	3232	3250	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1314008	.	sequence_feature	3049	3198	.	+	0	ID=BBa_K1314002;Name=BBa_K1314002;Parent=BBa_K1314004
BBa_K1314008	.	engineered_region	3049	3198	.	+	0	ID=annotation2397292;Name=random sequence/spacer;Parent=BBa_K1314002
>BBa_K1314008
tgtcagttttgtcacagggggccggaccaggatggtggacgctcgatggggatgtcgggccattgttcggttgtagcaa
ttacaacagtcggagtagggggattgtagggggattgttgtgtatcagaccgccctgctgctcccgtcgatggataatt
aatcatttaaaatcaatggtttatttatgtgttgcgggtgctggcacagacgctgcattacctactagagtcacacagg
aaacctactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacac
tctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagca
cgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcacta
ccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagc
cttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagt
gctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatcc
gtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagc
atttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaa
gactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatg
ctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgc
gctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctat
tgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttaca
tgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaa
cgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaa
gacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgca
agtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttccctta
caacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgctt
acgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcg
ataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccact
ggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcac
cacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgc
tccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaa
gaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacact
ggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtg
tgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccat
tcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatt
tgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctg
ctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccc
tgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctacc
attaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaag
acggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactc
cttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgt
gatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccgg
ctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagggaattggggcccttgtggcctaaatccaaccg
cggatcagtcagggcgcggatcccctgcaacgtcgcgacgctagagatactctatccagcggtagctcgtgtggttcaa
agggtaagccgtcacgctggcttcggaggtctatctgctactagagtccctatcagtgatagagattgacatccctatc
agtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagtt
catgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccg
tacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgc
agttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaagg
tttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggt
gagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggtt
gggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaaga
cggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaacc
gacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcact
ccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata