LOCUS BBa_K1314013 4311 bp DNA linear 02-Jan-2026 DEFINITION nifK-XhoI-nifH promoter T7 expression system(strong) ACCESSION BBa_K1314013 VERSION BBa_K1314013.1 FEATURES Location/Qualifiers misc_feature 1..170 /label=part of nifK misc_feature 171..176 /label=XhoI misc_feature 1..176 /label=BBa_K1314012 promoter 185..404 /label=nifH promoter promoter 185..404 /label=BBa_K1314000 misc_feature 417..420 /label=conserved RBS 413..424 /label=BBa_B0034 misc_feature 431..433 /label=start misc_feature 3080..3082 /label=stop misc_feature 3083..3085 /label=stop CDS 434..3079 /label=protein CDS 431..3085 /label=BBa_K145001 stem_loop 3105..3148 /label=stem_loop misc_feature 3094..3173 /label=BBa_B0010 terminator 3094..3173 /label=BBa_B0010 stem_loop 3189..3208 /label=T7 TE misc_feature 3215..3215 /label=stop polyA_site 3209..3222 /label=polya misc_feature 3182..3222 /label=BBa_B0012 terminator 3182..3222 /label=BBa_B0012 terminator 3094..3222 /label=BBa_B0015 misc_feature 185..3222 /label=BBa_K1314005 misc_feature 3231..3380 /label=random sequence/spacer misc_feature 3231..3380 /label=BBa_K1314002 misc_feature 3389..3407 /label=TetR 1 misc_feature 3389..3442 /label=BBa_R0040 promoter 3408..3413 /label=-35 misc_feature 3414..3432 /label=TetR 2 promoter 3431..3436 /label=-10 promoter 3389..3442 /label=BBa_R0040 misc_feature 3455..3458 /label=conserved RBS 3451..3462 /label=BBa_B0034 CDS 3469..4143 /label=mrfp1 misc_feature 4150..4174 /label=Help:Barcodes CDS 3469..4149 /label=BBa_E1010 stem_loop 4194..4237 /label=stem_loop misc_feature 4183..4262 /label=BBa_B0010 terminator 4183..4262 /label=BBa_B0010 stem_loop 4278..4297 /label=T7 TE misc_feature 4304..4304 /label=stop polyA_site 4298..4311 /label=polya misc_feature 4271..4311 /label=BBa_B0012 terminator 4271..4311 /label=BBa_B0012 misc_feature 3389..4311 /label=BBa_I13521 misc_feature 3231..4311 /label=BBa_K1314004 misc_feature 185..4311 /label=BBa_K1314007 ORIGIN 1 gtatcggctt cccgatcttc gaccgtcatc acctgcatcg ctccaccacc ctgggttacg 61 agggcgccat gcagatcctg accaccctgg tgaactcgat cctggaacgt ctggacgagg 121 aaacccgcgg tatgcaggcc accgactaca accacgacct ggtacgctaa ctcgagtact 181 agagtgtcag ttttgtcaca gggggccgga ccaggatggt ggacgctcga tggggatgtc 241 gggccattgt tcggttgtag caattacaac agtcggagta gggggattgt agggggattg 301 ttgtgtatca gaccgccctg ctgctcccgt cgatggataa ttaatcattt aaaatcaatg 361 gtttatttat gtgttgcggg tgctggcaca gacgctgcat tacctactag agaaagagga 421 gaaatactag atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc 481 tgctatcccg ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt 541 ggcccttgag catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg 601 tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct 661 actccctaag atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg 721 caagcgcccg acagccttcc agttcctgca agaaatcaag ccggaagccg tagcgtacat 781 caccattaag accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt 841 agcaagcgca atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct 901 tgaagctaag cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt 961 ctacaagaaa gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg 1021 tggcgaggcg tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat 1081 cgagatgctc attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt 1141 aggtcaagac tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg 1201 tgcaggtgcg ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc 1261 gtggactggc attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt 1321 gcgtactcac agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta 1381 caaagcgatt aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tcctagcggt 1441 cgccaacgta atcaccaagt ggaagcattg tccggtcgag gacatccctg cgattgagcg 1501 tgaagaactc ccgatgaaac cggaagacat cgacatgaat cctgaggctc tcaccgcgtg 1561 gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag 1621 ccttgagttc atgcttgagc aagccaataa gtttgctaac cataaggcca tctggttccc 1681 ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa 1741 cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta 1801 ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc cgttccctga 1861 gcgcatcaag ttcattgagg aaaaccacga gaacatcatg gcttgcgcta agtctccact 1921 ggagaacact tggtgggctg agcaagattc tccgttctgc ttccttgcgt tctgctttga 1981 gtacgctggg gtacagcacc acggcctgag ctataactgc tcccttccgc tggcgtttga 2041 cgggtcttgc tctggcatcc agcacttctc cgcgatgctc cgagatgagg taggtggtcg 2101 cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa 2161 agtcaacgag attctacaag cagacgcaat caatgggacc gataacgaag tagttaccgt 2221 gaccgatgag aacactggtg aaatctctga gaaagtcaag ctgggcacta aggcactggc 2281 tggtcaatgg ctggcttacg gtgttactcg cagtgtgact aagcgttcag tcatgacgct 2341 ggcttacggg tccaaagagt tcggcttccg tcaacaagtg ctggaagata ccattcagcc 2401 agctattgat tccggcaagg gtctgatgtt cactcagccg aatcaggctg ctggatacat 2461 ggctaagctg atttgggaat ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa 2521 ctggcttaag tctgctgcta agctgctggc tgctgaggtc aaagataaga agactggaga 2581 gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca 2641 ggaatacaag aagcctattc agacgcgctt gaacctgatg ttcctcggtc agttccgctt 2701 acagcctacc attaacacca acaaagatag cgagattgat gcacacaaac aggagtctgg 2761 tatcgctcct aactttgtac acagccaaga cggtagccac cttcgtaaga ctgtagtgtg 2821 ggcacacgag aagtacggaa tcgaatcttt tgcactgatt cacgactcct tcggtaccat 2881 tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg acacatatga 2941 gtcttgtgat gtactggctg atttctacga ccagttcgct gaccagttgc acgagtctca 3001 attggacaaa atgccagcac ttccggctaa aggtaacttg aacctccgtg acatcttaga 3061 gtcggacttc gcgttcgcgt aataatacta gagccaggca tcaaataaaa cgaaaggctc 3121 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3181 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag ggaattgggg 3241 cccttgtggc ctaaatccaa ccgcggatca gtcagggcgc ggatcccctg caacgtcgcg 3301 acgctagaga tactctatcc agcggtagct cgtgtggttc aaagggtaag ccgtcacgct 3361 ggcttcggag gtctatctgc tactagagtc cctatcagtg atagagattg acatccctat 3421 cagtgataga gatactgagc actactagag aaagaggaga aatactagat ggcttcctcc 3481 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt 3541 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct 3601 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag 3661 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa 3721 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt 3781 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg 3841 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa 3901 gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt 3961 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa 4021 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac 4081 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt 4141 gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac 4201 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 4261 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //