##gff-version 3
##sequence-region BBa_K1314013 1 4311
BBa_K1314013	.	sequence_feature	1	176	.	+	0	ID=BBa_K1314012;Name=BBa_K1314012
BBa_K1314013	.	sequence_feature	1	170	.	+	0	ID=annotation2397839;Name=part of nifK;Parent=BBa_K1314012
BBa_K1314013	.	sequence_feature	171	176	.	+	0	ID=annotation2397840;Name=XhoI;Parent=BBa_K1314012
BBa_K1314013	.	engineered_region	185	4311	.	+	0	ID=BBa_K1314007;Name=BBa_K1314007
BBa_K1314013	.	engineered_region	185	3222	.	+	0	ID=BBa_K1314005;Name=BBa_K1314005;Parent=BBa_K1314007
BBa_K1314013	.	ribosome_entry_site	413	424	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1314005
BBa_K1314013	.	conserved_region	417	420	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1314013	.	promoter	185	404	.	+	0	ID=BBa_K1314000;Name=BBa_K1314000;Parent=BBa_K1314005
BBa_K1314013	.	promoter	185	404	.	+	0	ID=annotation2396224;Name=nifH promoter;Parent=BBa_K1314000
BBa_K1314013	.	terminator	3094	3222	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1314005
BBa_K1314013	.	terminator	3182	3222	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1314013	.	polyA_site	3209	3222	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1314013	.	stem_loop	3189	3208	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1314013	.	engineered_region	3182	3222	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1314013	.	stop_codon	3215	3215	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1314013	.	terminator	3094	3173	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1314013	.	stem_loop	3105	3148	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1314013	.	engineered_region	3094	3173	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1314013	.	CDS	431	3085	.	+	0	ID=BBa_K145001;Name=BBa_K145001;Parent=BBa_K1314005
BBa_K1314013	.	stop_codon	3083	3085	.	+	0	ID=annotation1968218;Name=stop;Parent=BBa_K145001
BBa_K1314013	.	CDS	434	3079	.	+	0	ID=annotation1968243;Name=protein;Parent=BBa_K145001
BBa_K1314013	.	stop_codon	3080	3082	.	+	0	ID=annotation1968217;Name=stop;Parent=BBa_K145001
BBa_K1314013	.	start_codon	431	433	.	+	0	ID=annotation1968216;Name=start;Parent=BBa_K145001
BBa_K1314013	.	engineered_region	3231	4311	.	+	0	ID=BBa_K1314004;Name=BBa_K1314004;Parent=BBa_K1314007
BBa_K1314013	.	engineered_region	3389	4311	.	+	0	ID=BBa_I13521;Name=BBa_I13521;Parent=BBa_K1314004
BBa_K1314013	.	terminator	4183	4262	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_K1314013	.	stem_loop	4194	4237	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1314013	.	engineered_region	4183	4262	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1314013	.	CDS	3469	4149	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_K1314013	.	engineered_tag	4150	4174	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1314013	.	CDS	3469	4143	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1314013	.	terminator	4271	4311	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_K1314013	.	polyA_site	4298	4311	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1314013	.	stem_loop	4278	4297	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1314013	.	engineered_region	4271	4311	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1314013	.	stop_codon	4304	4304	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1314013	.	promoter	3389	3442	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_K1314013	.	promoter	3431	3436	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1314013	.	non_covalent_binding_site	3389	3407	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1314013	.	non_covalent_binding_site	3414	3432	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1314013	.	promoter	3408	3413	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1314013	.	engineered_region	3389	3442	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1314013	.	ribosome_entry_site	3451	3462	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_K1314013	.	conserved_region	3455	3458	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1314013	.	sequence_feature	3231	3380	.	+	0	ID=BBa_K1314002;Name=BBa_K1314002;Parent=BBa_K1314004
BBa_K1314013	.	engineered_region	3231	3380	.	+	0	ID=annotation2397292;Name=random sequence/spacer;Parent=BBa_K1314002
>BBa_K1314013
gtatcggcttcccgatcttcgaccgtcatcacctgcatcgctccaccaccctgggttacgagggcgccatgcagatcct
gaccaccctggtgaactcgatcctggaacgtctggacgaggaaacccgcggtatgcaggccaccgactacaaccacgac
ctggtacgctaactcgagtactagagtgtcagttttgtcacagggggccggaccaggatggtggacgctcgatggggat
gtcgggccattgttcggttgtagcaattacaacagtcggagtagggggattgtagggggattgttgtgtatcagaccgc
cctgctgctcccgtcgatggataattaatcatttaaaatcaatggtttatttatgtgttgcgggtgctggcacagacgc
tgcattacctactagagaaagaggagaaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcga
actggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcat
gagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacg
ctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagc
taagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaag
accactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgagg
acgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcg
cgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgag
gcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaa
tggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctga
ggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtgg
actggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcac
tgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaa
caagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgt
gaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctg
tgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaa
ccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggt
aacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatcc
acggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacat
catggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgc
tttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctg
gcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttca
ggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagta
gttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggc
tggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccg
tcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggct
gctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggctta
agtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgca
ttgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctc
ggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcg
ctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcga
atcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaact
atggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaat
tggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagggaattggg
gcccttgtggcctaaatccaaccgcggatcagtcagggcgcggatcccctgcaacgtcgcgacgctagagatactctat
ccagcggtagctcgtgtggttcaaagggtaagccgtcacgctggcttcggaggtctatctgctactagagtccctatca
gtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttc
ctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatc
gaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgt
tcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggacta
cctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacc
caggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccgg
ttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaat
caaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggtt
cagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagt
acgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttata