BBa_K1314013
type: ComponentDefinition
persistent identity: BBa K1314013
display id: BBa_K1314013
version: 1
was generated by: iGEM to SBOL conversion
description: nifK-XhoI-nifH promoter T7 expression system(strong)
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:09:48Z
mutable provenance: -
owned by: myers
owned by: james
mutable description: homologous integration knock out nitrogenase gene with nifH promoter T7 expression system
discontinued: false
dominant: false
experience: experience/None
group u list: _1689_
m user id: 0
owner id: 19949
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_K1314012
component: BBa_K1314007
sequence annotation: BBa_K1314012
sequence annotation: BBa_K1314007
sequence: https://synbiohub.org/public/igem/BBa_K1314013_sequence/1

BBa_K1314012
type: Component
persistent identity: component2397843
display id: component2397843
version: 1
top level: BBa_K1314013
definition: BBa_K1314012
access: public

BBa_K1314007
type: Component
persistent identity: component2397881
display id: component2397881
version: 1
top level: BBa_K1314013
definition: BBa_K1314007
access: public

BBa_K1314012
type: SequenceAnnotation
persistent identity: annotation2397843
display id: annotation2397843
version: 1
top level: BBa_K1314013
location: https://synbiohub.org/public/igem/BBa_K1314013/annotation2397843/range2397843/1
component: BBa_K1314012

https://synbiohub.org/public/igem/BBa_K1314013/annotation2397843/range2397843/1
type: Range
persistent identity: range2397843
display id: range2397843
version: 1
top level: BBa_K1314013
direction: direction/reverse
start: 1
end: 176
orientation: inline

BBa_K1314007
type: SequenceAnnotation
persistent identity: annotation2397881
display id: annotation2397881
version: 1
top level: BBa_K1314013
location: https://synbiohub.org/public/igem/BBa_K1314013/annotation2397881/range2397881/1
component: BBa_K1314007

https://synbiohub.org/public/igem/BBa_K1314013/annotation2397881/range2397881/1
type: Range
persistent identity: range2397881
display id: range2397881
version: 1
top level: BBa_K1314013
direction: direction/reverse
start: 185
end: 4311
orientation: inline

BBa_K1314007
type: ComponentDefinition
persistent identity: BBa K1314007
display id: BBa_K1314007
version: 1
was generated by: iGEM to SBOL conversion
description: nifH promoter+B0034+T7 RNA polymerase+ B0015+ random sequence+ptet mRFP reporter
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:09:48Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: nifH promoter+B0034+T7 RNA polymerase+ B0015+ random sequence+ptet mRFP reporter
discontinued: false
dominant: false
experience: experience/None
group u list: _1689_
m user id: 0
owner id: 19949
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_K1314004
component: BBa_K1314005
sequence annotation: BBa_K1314005
sequence annotation: BBa_K1314004
sequence: https://synbiohub.org/public/igem/BBa_K1314007_sequence/1

BBa_K1314004
type: Component
persistent identity: component2397754
display id: component2397754
version: 1
top level: BBa_K1314007
definition: BBa_K1314004
access: public

BBa_K1314005
type: Component
persistent identity: component2397734
display id: component2397734
version: 1
top level: BBa_K1314007
definition: BBa_K1314005
access: public

BBa_K1314005
type: SequenceAnnotation
persistent identity: annotation2397734
display id: annotation2397734
version: 1
top level: BBa_K1314007
location: https://synbiohub.org/public/igem/BBa_K1314007/annotation2397734/range2397734/1
component: BBa_K1314005

https://synbiohub.org/public/igem/BBa_K1314007/annotation2397734/range2397734/1
type: Range
persistent identity: range2397734
display id: range2397734
version: 1
top level: BBa_K1314007
direction: direction/reverse
start: 1
end: 3038
orientation: inline

BBa_K1314004
type: SequenceAnnotation
persistent identity: annotation2397754
display id: annotation2397754
version: 1
top level: BBa_K1314007
location: https://synbiohub.org/public/igem/BBa_K1314007/annotation2397754/range2397754/1
component: BBa_K1314004

https://synbiohub.org/public/igem/BBa_K1314007/annotation2397754/range2397754/1
type: Range
persistent identity: range2397754
display id: range2397754
version: 1
top level: BBa_K1314007
direction: direction/reverse
start: 3047
end: 4127
orientation: inline

BBa_K1314012
type: ComponentDefinition
persistent identity: BBa K1314012
display id: BBa_K1314012
version: 1
was generated by: iGEM to SBOL conversion
description: nif K homologous sequence
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:09:48Z
mutable provenance: Azotobacter vinelandii( strain DJ)
owned by: james
owned by: myers
mutable description: nif K homologous sequence
discontinued: false
dominant: false
experience: experience/None
group u list: _1689_
m user id: 0
owner id: 19949
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: part of nifK
sequence annotation: XhoI
sequence: https://synbiohub.org/public/igem/BBa_K1314012_sequence/1

part of nifK
type: SequenceAnnotation
persistent identity: annotation2397839
display id: annotation2397839
version: 1
top level: BBa_K1314012
location: https://synbiohub.org/public/igem/BBa_K1314012/annotation2397839/range2397839/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1314012/annotation2397839/range2397839/1
type: Range
persistent identity: range2397839
display id: range2397839
version: 1
top level: BBa_K1314012
direction: direction/forward
start: 1
end: 170
orientation: inline

XhoI
type: SequenceAnnotation
persistent identity: annotation2397840
display id: annotation2397840
version: 1
top level: BBa_K1314012
location: https://synbiohub.org/public/igem/BBa_K1314012/annotation2397840/range2397840/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1314012/annotation2397840/range2397840/1
type: Range
persistent identity: range2397840
display id: range2397840
version: 1
top level: BBa_K1314012
direction: direction/forward
start: 171
end: 176
orientation: inline

BBa_K1314004
type: ComponentDefinition
persistent identity: BBa K1314004
display id: BBa_K1314004
version: 1
was generated by: iGEM to SBOL conversion
description: Ptet mRFP with random sequence
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:09:48Z
mutable provenance: Composite of BBa_K1314002 and BBa_K1314003
owned by: james
owned by: myers
mutable description: Ptet prmoter mRFP with random sequence upstream
discontinued: false
dominant: false
experience: experience/None
group u list: _1689_
m user id: 0
owner id: 20058
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Ligation and Transformation
star: false
creator: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_I13521
component: BBa_K1314002
sequence annotation: BBa_K1314002
sequence annotation: BBa_I13521
sequence: https://synbiohub.org/public/igem/BBa_K1314004_sequence/1

BBa_I13521
type: Component
persistent identity: component2397657
display id: component2397657
version: 1
top level: BBa_K1314004
definition: BBa_I13521
access: public

BBa_K1314002
type: Component
persistent identity: component2397640
display id: component2397640
version: 1
top level: BBa_K1314004
definition: BBa_K1314002
access: public

BBa_K1314002
type: SequenceAnnotation
persistent identity: annotation2397640
display id: annotation2397640
version: 1
top level: BBa_K1314004
location: https://synbiohub.org/public/igem/BBa_K1314004/annotation2397640/range2397640/1
component: BBa_K1314002

https://synbiohub.org/public/igem/BBa_K1314004/annotation2397640/range2397640/1
type: Range
persistent identity: range2397640
display id: range2397640
version: 1
top level: BBa_K1314004
direction: direction/reverse
start: 1
end: 150
orientation: inline

BBa_I13521
type: SequenceAnnotation
persistent identity: annotation2397657
display id: annotation2397657
version: 1
top level: BBa_K1314004
location: https://synbiohub.org/public/igem/BBa_K1314004/annotation2397657/range2397657/1
component: BBa_I13521

https://synbiohub.org/public/igem/BBa_K1314004/annotation2397657/range2397657/1
type: Range
persistent identity: range2397657
display id: range2397657
version: 1
top level: BBa_K1314004
direction: direction/reverse
start: 159
end: 1081
orientation: inline

BBa_K1314005
type: ComponentDefinition
persistent identity: BBa K1314005
display id: BBa_K1314005
version: 1
was generated by: iGEM to SBOL conversion
description: nifH promoter+ B0034+T7 RNA polymerase+ B0015
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:09:48Z
mutable provenance: IGEM distribution kit
owned by: james
owned by: myers
mutable description: B0034+T7 RNA polymerase+ B0015
discontinued: false
dominant: false
experience: experience/None
group u list: _1689_
m user id: 0
owner id: 19949
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: ~~
star: false
creator: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0015
component: BBa_K145001
component: BBa_B0034
component: BBa_K1314000
sequence annotation: BBa_K1314000
sequence annotation: BBa_B0034
sequence annotation: BBa_K145001
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1314005_sequence/1

BBa_B0015
type: Component
persistent identity: component2397717
display id: component2397717
version: 1
top level: BBa_K1314005
definition: BBa_B0015
access: public

BBa_K145001
type: Component
persistent identity: component2397710
display id: component2397710
version: 1
top level: BBa_K1314005
definition: T7pol
access: public

BBa_B0034
type: Component
persistent identity: component2397705
display id: component2397705
version: 1
top level: BBa_K1314005
definition: BBa_B0034
access: public

BBa_K1314000
type: Component
persistent identity: component2397703
display id: component2397703
version: 1
top level: BBa_K1314005
definition: BBa_K1314000
access: public

BBa_K1314000
type: SequenceAnnotation
persistent identity: annotation2397703
display id: annotation2397703
version: 1
top level: BBa_K1314005
location: https://synbiohub.org/public/igem/BBa_K1314005/annotation2397703/range2397703/1
component: BBa_K1314000

https://synbiohub.org/public/igem/BBa_K1314005/annotation2397703/range2397703/1
type: Range
persistent identity: range2397703
display id: range2397703
version: 1
top level: BBa_K1314005
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2397705
display id: annotation2397705
version: 1
top level: BBa_K1314005
location: https://synbiohub.org/public/igem/BBa_K1314005/annotation2397705/range2397705/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1314005/annotation2397705/range2397705/1
type: Range
persistent identity: range2397705
display id: range2397705
version: 1
top level: BBa_K1314005
direction: direction/reverse
start: 229
end: 240
orientation: inline

BBa_K145001
type: SequenceAnnotation
persistent identity: annotation2397710
display id: annotation2397710
version: 1
top level: BBa_K1314005
location: https://synbiohub.org/public/igem/BBa_K1314005/annotation2397710/range2397710/1
component: BBa_K145001

https://synbiohub.org/public/igem/BBa_K1314005/annotation2397710/range2397710/1
type: Range
persistent identity: range2397710
display id: range2397710
version: 1
top level: BBa_K1314005
direction: direction/reverse
start: 247
end: 2901
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2397717
display id: annotation2397717
version: 1
top level: BBa_K1314005
location: https://synbiohub.org/public/igem/BBa_K1314005/annotation2397717/range2397717/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1314005/annotation2397717/range2397717/1
type: Range
persistent identity: range2397717
display id: range2397717
version: 1
top level: BBa_K1314005
direction: direction/reverse
start: 2910
end: 3038
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_I13521
type: ComponentDefinition
persistent identity: BBa I13521
display id: BBa_I13521
version: 1
was generated by: iGEM to SBOL conversion
description: Ptet mRFP
created: 2005-06-21T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive on mRFP (positive control)
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_R0040
component: BBa_E1010
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13521_sequence/1

BBa_R0040
type: Component
persistent identity: component1532558
display id: component1532558
version: 1
top level: BBa_I13521
definition: p(tetR)
access: public

BBa_E1010
type: Component
persistent identity: component1532573
display id: component1532573
version: 1
top level: BBa_I13521
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component1532566
display id: component1532566
version: 1
top level: BBa_I13521
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1532589
display id: component1532589
version: 1
top level: BBa_I13521
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1532579
display id: component1532579
version: 1
top level: BBa_I13521
definition: BBa_B0010
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1532558
display id: annotation1532558
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
type: Range
persistent identity: range1532558
display id: range1532558
version: 1
top level: BBa_I13521
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1532566
display id: annotation1532566
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532566/range1532566/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13521/annotation1532566/range1532566/1
type: Range
persistent identity: range1532566
display id: range1532566
version: 1
top level: BBa_I13521
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1532573
display id: annotation1532573
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
type: Range
persistent identity: range1532573
display id: range1532573
version: 1
top level: BBa_I13521
direction: direction/forward
start: 81
end: 761
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1532579
display id: annotation1532579
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
type: Range
persistent identity: range1532579
display id: range1532579
version: 1
top level: BBa_I13521
direction: direction/forward
start: 795
end: 874
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1532589
display id: annotation1532589
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
type: Range
persistent identity: range1532589
display id: range1532589
version: 1
top level: BBa_I13521
direction: direction/forward
start: 883
end: 923
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

T7pol
type: ComponentDefinition
persistent identity: BBa K145001
display id: BBa_K145001
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA polymerase
created: 2008-07-07T11:00:00Z
modified: 2015-05-08T01:10:28Z
mutable provenance: BBa_I712022 LVA tag from BBa_E0022
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 RNA polymerase with LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Fuse the LVA tag to the T7 coding region
star: true
creator: Jonas Demeulemeester and Benjamien Moeyaert
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: protein
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K145001_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1968216
display id: annotation1968216
version: 1
top level: T7pol
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968216/range1968216/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K145001/annotation1968216/range1968216/1
type: Range
persistent identity: range1968216
display id: range1968216
version: 1
top level: T7pol
direction: direction/forward
start: 1
end: 3
orientation: inline

protein
type: SequenceAnnotation
persistent identity: annotation1968243
display id: annotation1968243
version: 1
top level: T7pol
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968243/range1968243/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K145001/annotation1968243/range1968243/1
type: Range
persistent identity: range1968243
display id: range1968243
version: 1
top level: T7pol
direction: direction/forward
start: 4
end: 2649
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968217
display id: annotation1968217
version: 1
top level: T7pol
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968217/range1968217/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K145001/annotation1968217/range1968217/1
type: Range
persistent identity: range1968217
display id: range1968217
version: 1
top level: T7pol
direction: direction/forward
start: 2650
end: 2652
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968218
display id: annotation1968218
version: 1
top level: T7pol
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968218/range1968218/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K145001/annotation1968218/range1968218/1
type: Range
persistent identity: range1968218
display id: range1968218
version: 1
top level: T7pol
direction: direction/forward
start: 2653
end: 2655
orientation: inline

BBa_K1314000
type: ComponentDefinition
persistent identity: BBa K1314000
display id: BBa_K1314000
version: 1
was generated by: iGEM to SBOL conversion
description: Ammonium repressible nifH promoter(from Azotobacter vinelandii)
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:09:47Z
mutable provenance: A. vinelandii(strain DJ)
owned by: james
owned by: myers
mutable description: This part arose from the natural version of the A. vinelandii nifH promoter with illegal restriction site removed. It is part of the transcriptional regulatory system of the nitrogenase of A. vinelandii. The expression level of the gene under this promoter was found to be highest when nitrogen is depleted in A. vinelandii.(Trinity L. et al. , 2011) With a sufficiently low nitrogen level, the expression level from nifH promoter protein expression system is expected to be higher than that in tranditional E. coli T7 protein expression system. (2)
discontinued: false
dominant: false
experience: experience/None
group u list: _1689_
m user id: 0
owner id: 19949
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: nifH promoter
sequence: https://synbiohub.org/public/igem/BBa_K1314000_sequence/1

nifH promoter
type: SequenceAnnotation
persistent identity: annotation2396224
display id: annotation2396224
version: 1
top level: BBa_K1314000
location: https://synbiohub.org/public/igem/BBa_K1314000/annotation2396224/range2396224/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1314000/annotation2396224/range2396224/1
type: Range
persistent identity: range2396224
display id: range2396224
version: 1
top level: BBa_K1314000
direction: direction/forward
start: 1
end: 220
orientation: inline

BBa_K1314002
type: ComponentDefinition
persistent identity: BBa K1314002
display id: BBa_K1314002
version: 1
was generated by: iGEM to SBOL conversion
description: 150bp random sequence (spacer)
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:09:48Z
mutable provenance: synthesis by overlapping pcr
owned by: james
owned by: myers
mutable description: A random sequence for stable integration of azotobacter vinelandii.
discontinued: false
dominant: false
experience: experience/None
group u list: _1689_
m user id: 0
owner id: 20058
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: synthesis
star: false
creator: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: random sequence/spacer
sequence: https://synbiohub.org/public/igem/BBa_K1314002_sequence/1

random sequence/spacer
type: SequenceAnnotation
persistent identity: annotation2397292
display id: annotation2397292
version: 1
top level: BBa_K1314002
location: https://synbiohub.org/public/igem/BBa_K1314002/annotation2397292/range2397292/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1314002/annotation2397292/range2397292/1
type: Range
persistent identity: range2397292
display id: range2397292
version: 1
top level: BBa_K1314002
direction: direction/forward
start: 1
end: 150
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K1314013_sequence/1
type: Sequence
persistent identity: BBa K1314013 sequence
display id: BBa_K1314013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtatcggcttcccgatcttcgaccgtcatcacctgcatcgctccaccaccctgggttacgagggcgccatgcagatcctgaccaccctggtgaactcgatcctggaacgtctggacgaggaaacccgcggtatgcaggccaccgactacaaccacgacctggtacgctaactcgagtactagagtgtcagttttgtcacagggggccggaccaggatggtggacgctcgatggggatgtcgggccattgttcggttgtagcaattacaacagtcggagtagggggattgtagggggattgttgtgtatcagaccgccctgctgctcccgtcgatggataattaatcatttaaaatcaatggtttatttatgtgttgcgggtgctggcacagacgctgcattacctactagagaaagaggagaaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1314007_sequence/1
type: Sequence
persistent identity: BBa K1314007 sequence
display id: BBa_K1314007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtcagttttgtcacagggggccggaccaggatggtggacgctcgatggggatgtcgggccattgttcggttgtagcaattacaacagtcggagtagggggattgtagggggattgttgtgtatcagaccgccctgctgctcccgtcgatggataattaatcatttaaaatcaatggtttatttatgtgttgcgggtgctggcacagacgctgcattacctactagagaaagaggagaaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1314012_sequence/1
type: Sequence
persistent identity: BBa K1314012 sequence
display id: BBa_K1314012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtatcggcttcccgatcttcgaccgtcatcacctgcatcgctccaccaccctgggttacgagggcgccatgcagatcctgaccaccctggtgaactcgatcctggaacgtctggacgaggaaacccgcggtatgcaggccaccgactacaaccacgacctggtacgctaactcgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1314004_sequence/1
type: Sequence
persistent identity: BBa K1314004 sequence
display id: BBa_K1314004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggaattggggcccttgtggcctaaatccaaccgcggatcagtcagggcgcggatcccctgcaacgtcgcgacgctagagatactctatccagcggtagctcgtgtggttcaaagggtaagccgtcacgctggcttcggaggtctatctgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1314005_sequence/1
type: Sequence
persistent identity: BBa K1314005 sequence
display id: BBa_K1314005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtcagttttgtcacagggggccggaccaggatggtggacgctcgatggggatgtcgggccattgttcggttgtagcaattacaacagtcggagtagggggattgtagggggattgttgtgtatcagaccgccctgctgctcccgtcgatggataattaatcatttaaaatcaatggtttatttatgtgttgcgggtgctggcacagacgctgcattacctactagagaaagaggagaaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13521_sequence/1
type: Sequence
persistent identity: BBa I13521 sequence
display id: BBa_I13521_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145001_sequence/1
type: Sequence
persistent identity: BBa K145001 sequence
display id: BBa_K145001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1314000_sequence/1
type: Sequence
persistent identity: BBa K1314000 sequence
display id: BBa_K1314000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtcagttttgtcacagggggccggaccaggatggtggacgctcgatggggatgtcgggccattgttcggttgtagcaattacaacagtcggagtagggggattgtagggggattgttgtgtatcagaccgccctgctgctcccgtcgatggataattaatcatttaaaatcaatggtttatttatgtgttgcgggtgctggcacagacgctgcattacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1314002_sequence/1
type: Sequence
persistent identity: BBa K1314002 sequence
display id: BBa_K1314002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggaattggggcccttgtggcctaaatccaaccgcggatcagtcagggcgcggatcccctgcaacgtcgcgacgctagagatactctatccagcggtagctcgtgtggttcaaagggtaagccgtcacgctggcttcggaggtctatctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z