nifK-XhoI-nifH promoter T7 expression system(weak)
homologous integration knock out nitrogenase gene with nifH promoter T7 expression system
Seak Chi U, Chan Wai Lun, CHIU, Yee Ting
nifH promoter+B0031+T7 RNA polymerase+ B0015+ random sequence+ptet mRFP reporter
Composite of BBa_K1314002 and BBa_K1314003
Ptet prmoter mRFP with random sequence upstream
nifH promoter+B0031+T7 RNA polymerase+ B0015
Double terminator consisting of BBa_B0010 and BBa_B0012
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Fuse the LVA tag to the T7 coding region
Jonas Demeulemeester and Benjamien Moeyaert
Ammonium repressible nifH promoter(from Azotobacter vinelandii)
This part arose from the natural version of the A. vinelandii nifH promoter with illegal restriction site removed. It is part of the transcriptional regulatory system of the nitrogenase of A. vinelandii. The expression level of the gene under this promoter was found to be highest when nitrogen is depleted in A. vinelandii.(Trinity L. et al. , 2011) With a sufficiently low nitrogen level, the expression level from nifH promoter protein expression system is expected to be higher than that in tranditional E. coli T7 protein expression system. (2)
A random sequence for stable integration of azotobacter vinelandii.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
Sequence for pTet inverting regulator driven by the TetR protein.
BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1