LOCUS BBa_K1315009 1674 bp DNA linear 22-Dec-2024 DEFINITION Pseudomonas AutoInducer 1 (PAI-1) Biosensor ACCESSION BBa_K1315009 VERSION BBa_K1315009.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 CDS 61..783 /label=BBa_C0179 misc_feature 837..854 /label=OP2 misc_feature 897..916 /label=OP1 promoter 908..913 /label=-35 promoter 931..936 /label=-10 promoter 792..948 /label=BBa_R0079 CDS 955..1674 /label=GFP protein CDS 955..1674 /label=BBa_E0040 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 atggccttgg ttgacggttt tcttgagctg gaacgctcaa gtggaaaatt ggagtggagc 121 gccatcctcc agaagatggc gagcgacctt ggattctcga agatcctgtt cggcctgttg 181 cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg gcaactaccc ggccgcctgg 241 cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc cgacggtcag tcactgtacc 301 cagagcgtac tgccgatttt ctgggaaccg tccatctacc agacgcgaaa gcagcacgag 361 ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc tgaccatgcc gctgcatggt 421 gctcgcggcg aactcggcgc gctgagcctc agcgtggaag cggaaaaccg ggccgaggcc 481 aaccgtttca tagagtcggt cctgccgacc ctgtggatgc tcaaggacta cgcactgcaa 541 agcggtgccg gactggcctt cgaacatccg gtcagcaaac cggtggttct gaccagccgg 601 gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca gttgggagat atcggttatc 661 tgcaactgct cggaagccaa tgtgaacttc catatgggaa atattcggcg gaagttcggt 721 gtgacctccc gccgcgtagc ggccattatg gccgttaatt tgggtcttat tactctctaa 781 taatactaga ggcccctcgc tgagcgcgtc ccggagctgg gggcaaccta gctgccacct 841 gcttttctgc tagctattcc agcgaaaaca tacagatttc cggcgaaatc aaggctacct 901 gccagttctg gcaggtttgg ccgcgggttc tttttggtac acgaaagcta ctagatgcgt 961 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 1021 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt 1081 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 1141 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac 1201 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat 1261 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 1321 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 1381 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa 1441 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat 1501 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc 1561 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag 1621 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataa //