LOCUS       BBa_K1315010            1572 bp    DNA     linear       19-Apr-2024
DEFINITION  <i>Pseudomonas</i> AutoInducer 1 (PAI-1) Biosensor
ACCESSION   BBa_K1315010
VERSION     BBa_K1315010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             61..783
                     /label=BBa_C0179
     misc_feature    792..811
                     /label=LuxR/HSL
     promoter        811..816
                     /label=-35
     promoter        833..838
                     /label=-10
     misc_feature    844..844
                     /label=start
     misc_feature    792..846
                     /label=BBa_R0062
     promoter        792..846
                     /label=BBa_R0062
     CDS             853..1572
                     /label=GFP protein
     CDS             853..1572
                     /label=BBa_E0040
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 atggccttgg ttgacggttt tcttgagctg gaacgctcaa gtggaaaatt ggagtggagc
      121 gccatcctcc agaagatggc gagcgacctt ggattctcga agatcctgtt cggcctgttg
      181 cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg gcaactaccc ggccgcctgg
      241 cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc cgacggtcag tcactgtacc
      301 cagagcgtac tgccgatttt ctgggaaccg tccatctacc agacgcgaaa gcagcacgag
      361 ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc tgaccatgcc gctgcatggt
      421 gctcgcggcg aactcggcgc gctgagcctc agcgtggaag cggaaaaccg ggccgaggcc
      481 aaccgtttca tagagtcggt cctgccgacc ctgtggatgc tcaaggacta cgcactgcaa
      541 agcggtgccg gactggcctt cgaacatccg gtcagcaaac cggtggttct gaccagccgg
      601 gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca gttgggagat atcggttatc
      661 tgcaactgct cggaagccaa tgtgaacttc catatgggaa atattcggcg gaagttcggt
      721 gtgacctccc gccgcgtagc ggccattatg gccgttaatt tgggtcttat tactctctaa
      781 taatactaga gacctgtagg atcgtacagg tttacgcaag aaaatggttt gttatagtcg
      841 aataaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      901 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      961 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     1021 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1081 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1141 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1201 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1261 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1321 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     1381 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1441 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1501 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     1561 tacaaataat aa
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