LOCUS       BBa_K1316005            1252 bp    DNA     linear       23-May-2024
DEFINITION  ybiJ promoter coupled to mKate2 reporter gene
ACCESSION   BBa_K1316005
VERSION     BBa_K1316005.1  
FEATURES             Location/Qualifiers
     promoter        1..408
                     /label=ybiJ promoter
     promoter        1..424
                     /label=BBa_K1316004
     CDS             451..1149
                     /label=mKate2
     misc_feature    451..453
                     /label=start codon
     misc_feature    1147..1149
                     /label=stop codon
     RBS             433..444
                     /label=B0034 RBS
     misc_feature    433..1149
                     /label=BBa_K1316000
     stem_loop       1165..1184
                     /label=T7 TE
     misc_feature    1191..1191
                     /label=stop
     polyA_site      1185..1198
                     /label=polya
     misc_feature    1158..1198
                     /label=BBa_B0012
     terminator      1158..1198
                     /label=BBa_B0012
     stem_loop       1219..1241
                     /label=stem_loop
     misc_feature    1207..1252
                     /label=BBa_B0011
     terminator      1207..1252
                     /label=BBa_B0011
     terminator      1158..1252
                     /label=BBa_B0014
     terminator      1158..1252
                     /label=BBa_K823017
     misc_feature    433..1252
                     /label=BBa_K1316001
ORIGIN
        1 gcatgcggta cccgcagagg ttcgaaagac agccagttac gttaactaca actggcgaga
       61 tgcatagcga gtataatttg tattttgcgt gtcattccgt gcctttaacg ctgctgtgac
      121 tgccacgcgg tataaaacaa gttcataagt acaacaaata aatggtttat cagtaggtta
      181 gatatcaatc aatttatttg aacaaggcgg tcaattctct tcgattttat ctctcgtaaa
      241 aaaacgtgat actcatcaca tcgacgaaac aacgtcactt atacaaaaat cacctgcgag
      301 agattaatta tgaaaactat caatactgtt gttgctgcta tggctctttc aactctgtca
      361 tttggcgtgt tcgcggcgga accggtaacg gcatcccagg cacagaaccg atagacatat
      421 gctatactag agaaagagga gaaatatacc atggtgagcg agctgattaa ggagaacatg
      481 cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag
      541 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggcggt cgagggcggc
      601 cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag caaaaccttc
      661 atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccccga gggcttcaca
      721 tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc
      781 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac
      841 ggccctgtga tgcagaagaa aacactcggc tgggaggcct ccaccgagac cctgtacccc
      901 gctgacggcg gcctggaagg cagagccgac atggccctga agctcgtggg cgggggccac
      961 ctgatctgca acttgaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg
     1021 cccggcgtct actatgtgga cagaagactg gaaagaatca aggaggccga caaagagacc
     1081 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg
     1141 cacagatgat actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     1201 ctagagagag aatataaaaa gccagattat taatccggct tttttattat tt
//