LOCUS       BBa_K1316006            2795 bp    DNA     linear       07-May-2025
DEFINITION  Rhamnose promoter coupled to N-genes
ACCESSION   BBa_K1316006
VERSION     BBa_K1316006.1  
FEATURES             Location/Qualifiers
     promoter        1..122
                     /label=Rhamnose promoter
     RBS             129..140
                     /label=RBS B0034 
     CDS             149..887
                     /label=nfsA
     misc_feature    152..170
                     /label=6xHis tag
     misc_feature    888..891
                     /label=stop
     RBS             897..909
                     /label=RBS B0034
     CDS             916..1585
                     /label=nfsB
     misc_feature    1585..1588
                     /label=stop
     misc_feature    919..937
                     /label=6xHis tag
     RBS             1600..1612
                     /label=RBS B0034
     CDS             1619..2732
                     /label=nemA
     misc_feature    2714..2732
                     /label=6xHis tag
     misc_feature    2733..2735
                     /label=stop
     misc_feature    2737..2795
                     /label=His terminator
     misc_feature    149..152
                     /label=start
     misc_feature    916..918
                     /label=start
     misc_feature    1619..1621
                     /label=start
ORIGIN
        1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
       61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg
      121 aacgaagctt aaagaggaga aagatcatat gcatcatcat catcatcata cgccaaccat
      181 tgaacttatt tgtggccatc gctccattcg ccatttcact gatgaaccca tttccgaagc
      241 gcagcgtgag gcgattatta acagcgcccg tgcgacgtcc agttccagtt ttttgcagtg
      301 cagtagcatt attcgcatta ccgacaaagc gttacgtgaa gaactggtga cgctgaccgg
      361 cgggcaaaaa cacgtagcgc aagcggcgga gttctgggtg ttctgtgccg actttaaccg
      421 ccatttacaa atctgtccgg atgctcagct cggcctggcg gaacaactgt tgctcggtgt
      481 cgttgatacg gcaatgatgg cgcagaatgc attaatcgca gcggaatcgc tgggattggg
      541 cggggtatat atcggcggcc tgcgcaataa tattgaagcg gtgacgaaac tgcttaaatt
      601 accgcagcat gttctgccgc tgtttgggct gtgccttggc tggcctgcgg ataatccgga
      661 tcttaagccg cgtttaccgg cctccatttt ggtgcatgaa aacagctatc aaccgctgga
      721 taaaggcgca ctggcgcagt atgacgagca actggcggaa tattacctca cccgtggcag
      781 caataatcgc cgggatacct ggagcgatca tatccgccga acaatcatta aagaaagccg
      841 cccatttatt ctggattatt tgcacaaaca gggttgggcg acgcgctaac taagtaaaaa
      901 gaggagaaag atcatatgca tcatcatcat catcatgata tcatttctgt cgccttaaag
      961 cgtcattcca ctaaggcatt tgatgccagc aaaaaactta ccccggaaca ggccgagcag
     1021 atcaaaacgc tactgcaata cagcccatcc agcaccaact cccagccgtg gcattttatt
     1081 gttgccagca cggaagaagg taaagcgcgt gttgccaaat ccgctgccgg taattacgtg
     1141 ttcaacgagc gtaaaatgct tgatgcctcg cacgtcgtgg tgttctgtgc aaaaaccgcg
     1201 atggacgatg tctggctgaa gctggttgtt gaccaggaag atgccgatgg ccgctttgcc
     1261 acgccggaag cgaaagccgc gaacgataaa ggtcgcaagt tcttcgctga tatgcaccgt
     1321 aaggatctgc atgatgatgc agagtggatg gcaaaacagg tttatctcaa cgtcggtaac
     1381 ttcctgctcg gcgtggcggc tctgggtctg gacgcggtac ccatcgaagg ttttgacgcc
     1441 gccatcctcg atgcagaatt tggtctgaaa gagaaaggct acaccagtct ggtggttgtt
     1501 ccggtaggtc atcacagcgt tgaagatttt aacgctacgc tgccgaaatc tcgtctgccg
     1561 caaaacatca ccttaaccga agtgtaacta agtaaaaaga ggagaaagat catatgtcat
     1621 ctgaaaaact gtattcccca ctgaaagtgg gcgcgatcac ggcggcaaac cgtattttta
     1681 tggcaccgct gacgcgtctg cgcagtattg aaccgggtga cattcctacc ccgttgatgg
     1741 cggaatacta tcgccaacgt gccagtgccg gtttgattat tagtgaagcc acgcaaattt
     1801 ctgcccaggc aaaaggatat gcaggtgcgc ctggcatcca tagtccggag caaattgccg
     1861 catggaaaaa aatcaccgct ggcgttcatg ctgaaaatgg tcatatggcc gtgcaactgt
     1921 ggcacaccgg acgcatttct cacgccagcc tgcaacctgg cggtcaggcc ccggtagcgc
     1981 cttcagcact tagcgcggga acacgtactt ctctgcgcga tgaaaatggt caggcgatcc
     2041 gtgttgaaac atccatgccg cgtgcgcttg aactggaaga gattccaggt atcgtcaatg
     2101 atttccgtca ggccattgct aacgcgcgtg aagccggttt tgatctggta gagctccact
     2161 ctgctcacgg ttatttgctg catcagttcc tttctccttc ttcaaaccat cgtaccgatc
     2221 agtacggcgg cagcgtggaa aatcgcgcac gtttggtact ggaagtggtc gatgccggga
     2281 ttgaagaatg gggtgccgat cgcattggca ttcgcgtttc accaatcggt actttccaga
     2341 acacagataa cggcccgaat gaagaagccg atgcactgta tctgattgaa caactgggta
     2401 aacgcggcat tgcttatctg catatgtcag aaccagattg ggcggggggt gaaccgtata
     2461 ctgatgcgtt ccgcgaaaaa gtacgcgccc gtttccacgg tccgattatc ggcgcaggtg
     2521 catacacagt agaaaaagct gaaacgctga tcggcaaagg gttaattgat gcggtggcat
     2581 ttggtcgtga ctggattgcg aacccggatc tggtcgcccg cttgcagcgc aaagctgagc
     2641 ttaacccaca gcgtgccgaa agtttctacg gtggcggcgc ggaaggctat accgattacc
     2701 cgacgttgca tcatcatcat catcattaac taagtaatcc ggcaaaaaag ggcaaggtgt
     2761 caccaccctg ccctttttct ttaaaaccga aaaga
//