LOCUS BBa_K1316007 3902 bp DNA linear 03-Apr-2025 DEFINITION yqjF promoter coupled to mKate2 reporter gene and Rhamnose promoter coupled to N-genes ACCESSION BBa_K1316007 VERSION BBa_K1316007.1 FEATURES Location/Qualifiers promoter 1..249 /label=yqjF promoter promoter 1..271 /label=BBa_K1316002 CDS 298..996 /label=mKate2 misc_feature 298..300 /label=start codon misc_feature 994..996 /label=stop codon RBS 280..291 /label=B0034 RBS misc_feature 280..996 /label=BBa_K1316000 stem_loop 1012..1031 /label=T7 TE misc_feature 1038..1038 /label=stop polyA_site 1032..1045 /label=polya misc_feature 1005..1045 /label=BBa_B0012 terminator 1005..1045 /label=BBa_B0012 stem_loop 1066..1088 /label=stem_loop misc_feature 1054..1099 /label=BBa_B0011 terminator 1054..1099 /label=BBa_B0011 terminator 1005..1099 /label=BBa_B0014 terminator 1005..1099 /label=BBa_K823017 misc_feature 280..1099 /label=BBa_K1316001 misc_feature 1..1099 /label=BBa_K1316003 promoter 1108..1229 /label=Rhamnose promoter RBS 1236..1247 /label=RBS B0034 CDS 1256..1994 /label=nfsA misc_feature 1259..1277 /label=6xHis tag misc_feature 1995..1998 /label=stop RBS 2004..2016 /label=RBS B0034 CDS 2023..2692 /label=nfsB misc_feature 2692..2695 /label=stop misc_feature 2026..2044 /label=6xHis tag RBS 2707..2719 /label=RBS B0034 CDS 2726..3839 /label=nemA misc_feature 3821..3839 /label=6xHis tag misc_feature 3840..3842 /label=stop misc_feature 3844..3902 /label=His terminator misc_feature 1256..1259 /label=start misc_feature 2023..2025 /label=start misc_feature 2726..2728 /label=start CDS 1108..3902 /label=BBa_K1316006 ORIGIN 1 ggtggtaccc ggttttggcg tatggagcgc ctggcatctg gttaaaacga ccctcaagca 61 gcaacagctt cgcggttaac ttccctctgg ccggagccat tccggcctta tccctcaaat 121 tttttgaaga tctttgacaa ttttccttgc taacaatcat cattcaccac gtttatgatt 181 ctctccatcg acagcaacga cgctaatacc gcgcctttgc acaaaaaaac aatcagcagc 241 ctgagtggcc gagcatgcta gacatatgct atactagaga aagaggagaa atataccatg 301 gtgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac 361 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc 421 atgagaatca aggcggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc 481 agcttcatgt acggcagcaa aaccttcatc aaccacaccc agggcatccc cgacttcttt 541 aagcagtcct tccccgaggg cttcacatgg gagagagtca ccacatacga agacgggggc 601 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag 661 atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg 721 gaggcctcca ccgagaccct gtaccccgct gacggcggcc tggaaggcag agccgacatg 781 gccctgaagc tcgtgggcgg gggccacctg atctgcaact tgaagaccac atacagatcc 841 aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacag aagactggaa 901 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga 961 tactgcgacc tccctagcaa actggggcac agatgatact agagtcacac tggctcacct 1021 tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa 1081 tccggctttt ttattatttt actagagcca caattcagca aattgtgaac atcatcacgt 1141 tcatctttcc ctggttgcca atggcccatt ttcctgtcag taacgagaag gtcgcgtatt 1201 caggcgcttt ttagactggt cgtaatgaac gaagcttaaa gaggagaaag atcatatgca 1261 tcatcatcat catcatacgc caaccattga acttatttgt ggccatcgct ccattcgcca 1321 tttcactgat gaacccattt ccgaagcgca gcgtgaggcg attattaaca gcgcccgtgc 1381 gacgtccagt tccagttttt tgcagtgcag tagcattatt cgcattaccg acaaagcgtt 1441 acgtgaagaa ctggtgacgc tgaccggcgg gcaaaaacac gtagcgcaag cggcggagtt 1501 ctgggtgttc tgtgccgact ttaaccgcca tttacaaatc tgtccggatg ctcagctcgg 1561 cctggcggaa caactgttgc tcggtgtcgt tgatacggca atgatggcgc agaatgcatt 1621 aatcgcagcg gaatcgctgg gattgggcgg ggtatatatc ggcggcctgc gcaataatat 1681 tgaagcggtg acgaaactgc ttaaattacc gcagcatgtt ctgccgctgt ttgggctgtg 1741 ccttggctgg cctgcggata atccggatct taagccgcgt ttaccggcct ccattttggt 1801 gcatgaaaac agctatcaac cgctggataa aggcgcactg gcgcagtatg acgagcaact 1861 ggcggaatat tacctcaccc gtggcagcaa taatcgccgg gatacctgga gcgatcatat 1921 ccgccgaaca atcattaaag aaagccgccc atttattctg gattatttgc acaaacaggg 1981 ttgggcgacg cgctaactaa gtaaaaagag gagaaagatc atatgcatca tcatcatcat 2041 catgatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagcaaa 2101 aaacttaccc cggaacaggc cgagcagatc aaaacgctac tgcaatacag cccatccagc 2161 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt 2221 gccaaatccg ctgccggtaa ttacgtgttc aacgagcgta aaatgcttga tgcctcgcac 2281 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgtct ggctgaagct ggttgttgac 2341 caggaagatg ccgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt 2401 cgcaagttct tcgctgatat gcaccgtaag gatctgcatg atgatgcaga gtggatggca 2461 aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggctct gggtctggac 2521 gcggtaccca tcgaaggttt tgacgccgcc atcctcgatg cagaatttgg tctgaaagag 2581 aaaggctaca ccagtctggt ggttgttccg gtaggtcatc acagcgttga agattttaac 2641 gctacgctgc cgaaatctcg tctgccgcaa aacatcacct taaccgaagt gtaactaagt 2701 aaaaagagga gaaagatcat atgtcatctg aaaaactgta ttccccactg aaagtgggcg 2761 cgatcacggc ggcaaaccgt atttttatgg caccgctgac gcgtctgcgc agtattgaac 2821 cgggtgacat tcctaccccg ttgatggcgg aatactatcg ccaacgtgcc agtgccggtt 2881 tgattattag tgaagccacg caaatttctg cccaggcaaa aggatatgca ggtgcgcctg 2941 gcatccatag tccggagcaa attgccgcat ggaaaaaaat caccgctggc gttcatgctg 3001 aaaatggtca tatggccgtg caactgtggc acaccggacg catttctcac gccagcctgc 3061 aacctggcgg tcaggccccg gtagcgcctt cagcacttag cgcgggaaca cgtacttctc 3121 tgcgcgatga aaatggtcag gcgatccgtg ttgaaacatc catgccgcgt gcgcttgaac 3181 tggaagagat tccaggtatc gtcaatgatt tccgtcaggc cattgctaac gcgcgtgaag 3241 ccggttttga tctggtagag ctccactctg ctcacggtta tttgctgcat cagttccttt 3301 ctccttcttc aaaccatcgt accgatcagt acggcggcag cgtggaaaat cgcgcacgtt 3361 tggtactgga agtggtcgat gccgggattg aagaatgggg tgccgatcgc attggcattc 3421 gcgtttcacc aatcggtact ttccagaaca cagataacgg cccgaatgaa gaagccgatg 3481 cactgtatct gattgaacaa ctgggtaaac gcggcattgc ttatctgcat atgtcagaac 3541 cagattgggc ggggggtgaa ccgtatactg atgcgttccg cgaaaaagta cgcgcccgtt 3601 tccacggtcc gattatcggc gcaggtgcat acacagtaga aaaagctgaa acgctgatcg 3661 gcaaagggtt aattgatgcg gtggcatttg gtcgtgactg gattgcgaac ccggatctgg 3721 tcgcccgctt gcagcgcaaa gctgagctta acccacagcg tgccgaaagt ttctacggtg 3781 gcggcgcgga aggctatacc gattacccga cgttgcatca tcatcatcat cattaactaa 3841 gtaatccggc aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa 3901 ga //