##gff-version 3
##sequence-region BBa_K1316007 1 3902
BBa_K1316007	.	engineered_region	1	1099	.	+	0	ID=BBa_K1316003;Name=BBa_K1316003
BBa_K1316007	.	engineered_region	280	1099	.	+	0	ID=BBa_K1316001;Name=BBa_K1316001;Parent=BBa_K1316003
BBa_K1316007	.	terminator	1005	1099	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1316001
BBa_K1316007	.	terminator	1005	1099	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1316007	.	terminator	1054	1099	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1316007	.	engineered_region	1054	1099	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1316007	.	stem_loop	1066	1088	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1316007	.	terminator	1005	1045	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1316007	.	stem_loop	1012	1031	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1316007	.	polyA_site	1032	1045	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1316007	.	stop_codon	1038	1038	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1316007	.	engineered_region	1005	1045	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1316007	.	engineered_region	280	996	.	+	0	ID=BBa_K1316000;Name=BBa_K1316000;Parent=BBa_K1316001
BBa_K1316007	.	start_codon	298	300	.	+	0	ID=annotation2381269;Name=start codon;Parent=BBa_K1316000
BBa_K1316007	.	stop_codon	994	996	.	+	0	ID=annotation2381270;Name=stop codon;Parent=BBa_K1316000
BBa_K1316007	.	CDS	298	996	.	+	0	ID=annotation2381268;Name=mKate2;Parent=BBa_K1316000
BBa_K1316007	.	ribosome_entry_site	280	291	.	+	0	ID=annotation2381284;Name=B0034 RBS;Parent=BBa_K1316000
BBa_K1316007	.	promoter	1	271	.	+	0	ID=BBa_K1316002;Name=BBa_K1316002;Parent=BBa_K1316003
BBa_K1316007	.	promoter	1	249	.	+	0	ID=annotation2381283;Name=yqjF promoter;Parent=BBa_K1316002
BBa_K1316007	.	CDS	1108	3902	.	+	0	ID=BBa_K1316006;Name=BBa_K1316006
BBa_K1316007	.	start_codon	1256	1259	.	+	0	ID=annotation2382958;Name=start;Parent=BBa_K1316006
BBa_K1316007	.	stop_codon	1995	1998	.	+	0	ID=annotation2382947;Name=stop;Parent=BBa_K1316006
BBa_K1316007	.	CDS	1256	1994	.	+	0	ID=annotation2382945;Name=nfsA;Parent=BBa_K1316006
BBa_K1316007	.	promoter	1108	1229	.	+	0	ID=annotation2382943;Name=Rhamnose promoter;Parent=BBa_K1316006
BBa_K1316007	.	tag	2026	2044	.	+	0	ID=annotation2382952;Name=6xHis tag;Parent=BBa_K1316006
BBa_K1316007	.	tag	3821	3839	.	+	0	ID=annotation2382955;Name=6xHis tag;Parent=BBa_K1316006
BBa_K1316007	.	sequence_feature	3844	3902	.	+	0	ID=annotation2382957;Name=His terminator;Parent=BBa_K1316006
BBa_K1316007	.	stop_codon	2692	2695	.	+	0	ID=annotation2382950;Name=stop;Parent=BBa_K1316006
BBa_K1316007	.	ribosome_entry_site	1236	1247	.	+	0	ID=annotation2382944;Name=RBS B0034 ;Parent=BBa_K1316006
BBa_K1316007	.	CDS	2726	3839	.	+	0	ID=annotation2382954;Name=nemA;Parent=BBa_K1316006
BBa_K1316007	.	start_codon	2023	2025	.	+	0	ID=annotation2382959;Name=start;Parent=BBa_K1316006
BBa_K1316007	.	stop_codon	3840	3842	.	+	0	ID=annotation2382956;Name=stop;Parent=BBa_K1316006
BBa_K1316007	.	ribosome_entry_site	2004	2016	.	+	0	ID=annotation2382948;Name=RBS B0034;Parent=BBa_K1316006
BBa_K1316007	.	start_codon	2726	2728	.	+	0	ID=annotation2382960;Name=start;Parent=BBa_K1316006
BBa_K1316007	.	ribosome_entry_site	2707	2719	.	+	0	ID=annotation2382953;Name=RBS B0034;Parent=BBa_K1316006
BBa_K1316007	.	tag	1259	1277	.	+	0	ID=annotation2382946;Name=6xHis tag;Parent=BBa_K1316006
BBa_K1316007	.	CDS	2023	2692	.	+	0	ID=annotation2382949;Name=nfsB;Parent=BBa_K1316006
>BBa_K1316007
ggtggtacccggttttggcgtatggagcgcctggcatctggttaaaacgaccctcaagcagcaacagcttcgcggttaa
cttccctctggccggagccattccggccttatccctcaaattttttgaagatctttgacaattttccttgctaacaatc
atcattcaccacgtttatgattctctccatcgacagcaacgacgctaataccgcgcctttgcacaaaaaaacaatcagc
agcctgagtggccgagcatgctagacatatgctatactagagaaagaggagaaatataccatggtgagcgagctgatta
aggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaagg
caagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctg
gctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttcc
ccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctcca
ggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaaca
ctcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagc
tcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcc
cggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtg
gctgtggccagatactgcgacctccctagcaaactggggcacagatgatactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactaga
gccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacg
agaaggtcgcgtattcaggcgctttttagactggtcgtaatgaacgaagcttaaagaggagaaagatcatatgcatcat
catcatcatcatacgccaaccattgaacttatttgtggccatcgctccattcgccatttcactgatgaacccatttccg
aagcgcagcgtgaggcgattattaacagcgcccgtgcgacgtccagttccagttttttgcagtgcagtagcattattcg
cattaccgacaaagcgttacgtgaagaactggtgacgctgaccggcgggcaaaaacacgtagcgcaagcggcggagttc
tgggtgttctgtgccgactttaaccgccatttacaaatctgtccggatgctcagctcggcctggcggaacaactgttgc
tcggtgtcgttgatacggcaatgatggcgcagaatgcattaatcgcagcggaatcgctgggattgggcggggtatatat
cggcggcctgcgcaataatattgaagcggtgacgaaactgcttaaattaccgcagcatgttctgccgctgtttgggctg
tgccttggctggcctgcggataatccggatcttaagccgcgtttaccggcctccattttggtgcatgaaaacagctatc
aaccgctggataaaggcgcactggcgcagtatgacgagcaactggcggaatattacctcacccgtggcagcaataatcg
ccgggatacctggagcgatcatatccgccgaacaatcattaaagaaagccgcccatttattctggattatttgcacaaa
cagggttgggcgacgcgctaactaagtaaaaagaggagaaagatcatatgcatcatcatcatcatcatgatatcatttc
tgtcgccttaaagcgtcattccactaaggcatttgatgccagcaaaaaacttaccccggaacaggccgagcagatcaaa
acgctactgcaatacagcccatccagcaccaactcccagccgtggcattttattgttgccagcacggaagaaggtaaag
cgcgtgttgccaaatccgctgccggtaattacgtgttcaacgagcgtaaaatgcttgatgcctcgcacgtcgtggtgtt
ctgtgcaaaaaccgcgatggacgatgtctggctgaagctggttgttgaccaggaagatgccgatggccgctttgccacg
ccggaagcgaaagccgcgaacgataaaggtcgcaagttcttcgctgatatgcaccgtaaggatctgcatgatgatgcag
agtggatggcaaaacaggtttatctcaacgtcggtaacttcctgctcggcgtggcggctctgggtctggacgcggtacc
catcgaaggttttgacgccgccatcctcgatgcagaatttggtctgaaagagaaaggctacaccagtctggtggttgtt
ccggtaggtcatcacagcgttgaagattttaacgctacgctgccgaaatctcgtctgccgcaaaacatcaccttaaccg
aagtgtaactaagtaaaaagaggagaaagatcatatgtcatctgaaaaactgtattccccactgaaagtgggcgcgatc
acggcggcaaaccgtatttttatggcaccgctgacgcgtctgcgcagtattgaaccgggtgacattcctaccccgttga
tggcggaatactatcgccaacgtgccagtgccggtttgattattagtgaagccacgcaaatttctgcccaggcaaaagg
atatgcaggtgcgcctggcatccatagtccggagcaaattgccgcatggaaaaaaatcaccgctggcgttcatgctgaa
aatggtcatatggccgtgcaactgtggcacaccggacgcatttctcacgccagcctgcaacctggcggtcaggccccgg
tagcgccttcagcacttagcgcgggaacacgtacttctctgcgcgatgaaaatggtcaggcgatccgtgttgaaacatc
catgccgcgtgcgcttgaactggaagagattccaggtatcgtcaatgatttccgtcaggccattgctaacgcgcgtgaa
gccggttttgatctggtagagctccactctgctcacggttatttgctgcatcagttcctttctccttcttcaaaccatc
gtaccgatcagtacggcggcagcgtggaaaatcgcgcacgtttggtactggaagtggtcgatgccgggattgaagaatg
gggtgccgatcgcattggcattcgcgtttcaccaatcggtactttccagaacacagataacggcccgaatgaagaagcc
gatgcactgtatctgattgaacaactgggtaaacgcggcattgcttatctgcatatgtcagaaccagattgggcggggg
gtgaaccgtatactgatgcgttccgcgaaaaagtacgcgcccgtttccacggtccgattatcggcgcaggtgcatacac
agtagaaaaagctgaaacgctgatcggcaaagggttaattgatgcggtggcatttggtcgtgactggattgcgaacccg
gatctggtcgcccgcttgcagcgcaaagctgagcttaacccacagcgtgccgaaagtttctacggtggcggcgcggaag
gctataccgattacccgacgttgcatcatcatcatcatcattaactaagtaatccggcaaaaaagggcaaggtgtcacc
accctgccctttttctttaaaaccgaaaaga