LOCUS BBa_K1316008 4055 bp DNA linear 22-Dec-2024 DEFINITION ybiJ promoter coupled to mKate2 reporter gene and Rhamnose promoter coupled to N-genes ACCESSION BBa_K1316008 VERSION BBa_K1316008.1 FEATURES Location/Qualifiers promoter 1..408 /label=ybiJ promoter promoter 1..424 /label=BBa_K1316004 CDS 451..1149 /label=mKate2 misc_feature 451..453 /label=start codon misc_feature 1147..1149 /label=stop codon RBS 433..444 /label=B0034 RBS misc_feature 433..1149 /label=BBa_K1316000 stem_loop 1165..1184 /label=T7 TE misc_feature 1191..1191 /label=stop polyA_site 1185..1198 /label=polya misc_feature 1158..1198 /label=BBa_B0012 terminator 1158..1198 /label=BBa_B0012 stem_loop 1219..1241 /label=stem_loop misc_feature 1207..1252 /label=BBa_B0011 terminator 1207..1252 /label=BBa_B0011 terminator 1158..1252 /label=BBa_B0014 terminator 1158..1252 /label=BBa_K823017 misc_feature 433..1252 /label=BBa_K1316001 misc_feature 1..1252 /label=BBa_K1316005 promoter 1261..1382 /label=Rhamnose promoter RBS 1389..1400 /label=RBS B0034 CDS 1409..2147 /label=nfsA misc_feature 1412..1430 /label=6xHis tag misc_feature 2148..2151 /label=stop RBS 2157..2169 /label=RBS B0034 CDS 2176..2845 /label=nfsB misc_feature 2845..2848 /label=stop misc_feature 2179..2197 /label=6xHis tag RBS 2860..2872 /label=RBS B0034 CDS 2879..3992 /label=nemA misc_feature 3974..3992 /label=6xHis tag misc_feature 3993..3995 /label=stop misc_feature 3997..4055 /label=His terminator misc_feature 1409..1412 /label=start misc_feature 2176..2178 /label=start misc_feature 2879..2881 /label=start CDS 1261..4055 /label=BBa_K1316006 ORIGIN 1 gcatgcggta cccgcagagg ttcgaaagac agccagttac gttaactaca actggcgaga 61 tgcatagcga gtataatttg tattttgcgt gtcattccgt gcctttaacg ctgctgtgac 121 tgccacgcgg tataaaacaa gttcataagt acaacaaata aatggtttat cagtaggtta 181 gatatcaatc aatttatttg aacaaggcgg tcaattctct tcgattttat ctctcgtaaa 241 aaaacgtgat actcatcaca tcgacgaaac aacgtcactt atacaaaaat cacctgcgag 301 agattaatta tgaaaactat caatactgtt gttgctgcta tggctctttc aactctgtca 361 tttggcgtgt tcgcggcgga accggtaacg gcatcccagg cacagaaccg atagacatat 421 gctatactag agaaagagga gaaatatacc atggtgagcg agctgattaa ggagaacatg 481 cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag 541 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggcggt cgagggcggc 601 cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag caaaaccttc 661 atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccccga gggcttcaca 721 tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc 781 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac 841 ggccctgtga tgcagaagaa aacactcggc tgggaggcct ccaccgagac cctgtacccc 901 gctgacggcg gcctggaagg cagagccgac atggccctga agctcgtggg cgggggccac 961 ctgatctgca acttgaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg 1021 cccggcgtct actatgtgga cagaagactg gaaagaatca aggaggccga caaagagacc 1081 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg 1141 cacagatgat actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 1201 ctagagagag aatataaaaa gccagattat taatccggct tttttattat tttactagag 1261 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc 1321 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg 1381 aacgaagctt aaagaggaga aagatcatat gcatcatcat catcatcata cgccaaccat 1441 tgaacttatt tgtggccatc gctccattcg ccatttcact gatgaaccca tttccgaagc 1501 gcagcgtgag gcgattatta acagcgcccg tgcgacgtcc agttccagtt ttttgcagtg 1561 cagtagcatt attcgcatta ccgacaaagc gttacgtgaa gaactggtga cgctgaccgg 1621 cgggcaaaaa cacgtagcgc aagcggcgga gttctgggtg ttctgtgccg actttaaccg 1681 ccatttacaa atctgtccgg atgctcagct cggcctggcg gaacaactgt tgctcggtgt 1741 cgttgatacg gcaatgatgg cgcagaatgc attaatcgca gcggaatcgc tgggattggg 1801 cggggtatat atcggcggcc tgcgcaataa tattgaagcg gtgacgaaac tgcttaaatt 1861 accgcagcat gttctgccgc tgtttgggct gtgccttggc tggcctgcgg ataatccgga 1921 tcttaagccg cgtttaccgg cctccatttt ggtgcatgaa aacagctatc aaccgctgga 1981 taaaggcgca ctggcgcagt atgacgagca actggcggaa tattacctca cccgtggcag 2041 caataatcgc cgggatacct ggagcgatca tatccgccga acaatcatta aagaaagccg 2101 cccatttatt ctggattatt tgcacaaaca gggttgggcg acgcgctaac taagtaaaaa 2161 gaggagaaag atcatatgca tcatcatcat catcatgata tcatttctgt cgccttaaag 2221 cgtcattcca ctaaggcatt tgatgccagc aaaaaactta ccccggaaca ggccgagcag 2281 atcaaaacgc tactgcaata cagcccatcc agcaccaact cccagccgtg gcattttatt 2341 gttgccagca cggaagaagg taaagcgcgt gttgccaaat ccgctgccgg taattacgtg 2401 ttcaacgagc gtaaaatgct tgatgcctcg cacgtcgtgg tgttctgtgc aaaaaccgcg 2461 atggacgatg tctggctgaa gctggttgtt gaccaggaag atgccgatgg ccgctttgcc 2521 acgccggaag cgaaagccgc gaacgataaa ggtcgcaagt tcttcgctga tatgcaccgt 2581 aaggatctgc atgatgatgc agagtggatg gcaaaacagg tttatctcaa cgtcggtaac 2641 ttcctgctcg gcgtggcggc tctgggtctg gacgcggtac ccatcgaagg ttttgacgcc 2701 gccatcctcg atgcagaatt tggtctgaaa gagaaaggct acaccagtct ggtggttgtt 2761 ccggtaggtc atcacagcgt tgaagatttt aacgctacgc tgccgaaatc tcgtctgccg 2821 caaaacatca ccttaaccga agtgtaacta agtaaaaaga ggagaaagat catatgtcat 2881 ctgaaaaact gtattcccca ctgaaagtgg gcgcgatcac ggcggcaaac cgtattttta 2941 tggcaccgct gacgcgtctg cgcagtattg aaccgggtga cattcctacc ccgttgatgg 3001 cggaatacta tcgccaacgt gccagtgccg gtttgattat tagtgaagcc acgcaaattt 3061 ctgcccaggc aaaaggatat gcaggtgcgc ctggcatcca tagtccggag caaattgccg 3121 catggaaaaa aatcaccgct ggcgttcatg ctgaaaatgg tcatatggcc gtgcaactgt 3181 ggcacaccgg acgcatttct cacgccagcc tgcaacctgg cggtcaggcc ccggtagcgc 3241 cttcagcact tagcgcggga acacgtactt ctctgcgcga tgaaaatggt caggcgatcc 3301 gtgttgaaac atccatgccg cgtgcgcttg aactggaaga gattccaggt atcgtcaatg 3361 atttccgtca ggccattgct aacgcgcgtg aagccggttt tgatctggta gagctccact 3421 ctgctcacgg ttatttgctg catcagttcc tttctccttc ttcaaaccat cgtaccgatc 3481 agtacggcgg cagcgtggaa aatcgcgcac gtttggtact ggaagtggtc gatgccggga 3541 ttgaagaatg gggtgccgat cgcattggca ttcgcgtttc accaatcggt actttccaga 3601 acacagataa cggcccgaat gaagaagccg atgcactgta tctgattgaa caactgggta 3661 aacgcggcat tgcttatctg catatgtcag aaccagattg ggcggggggt gaaccgtata 3721 ctgatgcgtt ccgcgaaaaa gtacgcgccc gtttccacgg tccgattatc ggcgcaggtg 3781 catacacagt agaaaaagct gaaacgctga tcggcaaagg gttaattgat gcggtggcat 3841 ttggtcgtga ctggattgcg aacccggatc tggtcgcccg cttgcagcgc aaagctgagc 3901 ttaacccaca gcgtgccgaa agtttctacg gtggcggcgc ggaaggctat accgattacc 3961 cgacgttgca tcatcatcat catcattaac taagtaatcc ggcaaaaaag ggcaaggtgt 4021 caccaccctg ccctttttct ttaaaaccga aaaga //