##gff-version 3
##sequence-region BBa_K1316008 1 4055
BBa_K1316008	.	CDS	1261	4055	.	+	0	ID=BBa_K1316006;Name=BBa_K1316006
BBa_K1316008	.	start_codon	1409	1412	.	+	0	ID=annotation2382958;Name=start;Parent=BBa_K1316006
BBa_K1316008	.	ribosome_entry_site	1389	1400	.	+	0	ID=annotation2382944;Name=RBS B0034 ;Parent=BBa_K1316006
BBa_K1316008	.	start_codon	2176	2178	.	+	0	ID=annotation2382959;Name=start;Parent=BBa_K1316006
BBa_K1316008	.	stop_codon	3993	3995	.	+	0	ID=annotation2382956;Name=stop;Parent=BBa_K1316006
BBa_K1316008	.	CDS	2176	2845	.	+	0	ID=annotation2382949;Name=nfsB;Parent=BBa_K1316006
BBa_K1316008	.	tag	1412	1430	.	+	0	ID=annotation2382946;Name=6xHis tag;Parent=BBa_K1316006
BBa_K1316008	.	CDS	2879	3992	.	+	0	ID=annotation2382954;Name=nemA;Parent=BBa_K1316006
BBa_K1316008	.	sequence_feature	3997	4055	.	+	0	ID=annotation2382957;Name=His terminator;Parent=BBa_K1316006
BBa_K1316008	.	ribosome_entry_site	2860	2872	.	+	0	ID=annotation2382953;Name=RBS B0034;Parent=BBa_K1316006
BBa_K1316008	.	tag	3974	3992	.	+	0	ID=annotation2382955;Name=6xHis tag;Parent=BBa_K1316006
BBa_K1316008	.	start_codon	2879	2881	.	+	0	ID=annotation2382960;Name=start;Parent=BBa_K1316006
BBa_K1316008	.	promoter	1261	1382	.	+	0	ID=annotation2382943;Name=Rhamnose promoter;Parent=BBa_K1316006
BBa_K1316008	.	stop_codon	2148	2151	.	+	0	ID=annotation2382947;Name=stop;Parent=BBa_K1316006
BBa_K1316008	.	tag	2179	2197	.	+	0	ID=annotation2382952;Name=6xHis tag;Parent=BBa_K1316006
BBa_K1316008	.	CDS	1409	2147	.	+	0	ID=annotation2382945;Name=nfsA;Parent=BBa_K1316006
BBa_K1316008	.	ribosome_entry_site	2157	2169	.	+	0	ID=annotation2382948;Name=RBS B0034;Parent=BBa_K1316006
BBa_K1316008	.	stop_codon	2845	2848	.	+	0	ID=annotation2382950;Name=stop;Parent=BBa_K1316006
BBa_K1316008	.	engineered_region	1	1252	.	+	0	ID=BBa_K1316005;Name=BBa_K1316005
BBa_K1316008	.	promoter	1	424	.	+	0	ID=BBa_K1316004;Name=BBa_K1316004;Parent=BBa_K1316005
BBa_K1316008	.	promoter	1	408	.	+	0	ID=annotation2381327;Name=ybiJ promoter;Parent=BBa_K1316004
BBa_K1316008	.	engineered_region	433	1252	.	+	0	ID=BBa_K1316001;Name=BBa_K1316001;Parent=BBa_K1316005
BBa_K1316008	.	terminator	1158	1252	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1316001
BBa_K1316008	.	terminator	1158	1252	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1316008	.	terminator	1158	1198	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1316008	.	polyA_site	1185	1198	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1316008	.	engineered_region	1158	1198	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1316008	.	stem_loop	1165	1184	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1316008	.	stop_codon	1191	1191	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1316008	.	terminator	1207	1252	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1316008	.	stem_loop	1219	1241	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1316008	.	engineered_region	1207	1252	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1316008	.	engineered_region	433	1149	.	+	0	ID=BBa_K1316000;Name=BBa_K1316000;Parent=BBa_K1316001
BBa_K1316008	.	start_codon	451	453	.	+	0	ID=annotation2381269;Name=start codon;Parent=BBa_K1316000
BBa_K1316008	.	CDS	451	1149	.	+	0	ID=annotation2381268;Name=mKate2;Parent=BBa_K1316000
BBa_K1316008	.	ribosome_entry_site	433	444	.	+	0	ID=annotation2381284;Name=B0034 RBS;Parent=BBa_K1316000
BBa_K1316008	.	stop_codon	1147	1149	.	+	0	ID=annotation2381270;Name=stop codon;Parent=BBa_K1316000
>BBa_K1316008
gcatgcggtacccgcagaggttcgaaagacagccagttacgttaactacaactggcgagatgcatagcgagtataattt
gtattttgcgtgtcattccgtgcctttaacgctgctgtgactgccacgcggtataaaacaagttcataagtacaacaaa
taaatggtttatcagtaggttagatatcaatcaatttatttgaacaaggcggtcaattctcttcgattttatctctcgt
aaaaaaacgtgatactcatcacatcgacgaaacaacgtcacttatacaaaaatcacctgcgagagattaattatgaaaa
ctatcaatactgttgttgctgctatggctctttcaactctgtcatttggcgtgttcgcggcggaaccggtaacggcatc
ccaggcacagaaccgatagacatatgctatactagagaaagaggagaaatataccatggtgagcgagctgattaaggag
aacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagc
cctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggctac
cagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgag
ggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacg
gctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcgg
ctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtg
ggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcg
tctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgt
ggccagatactgcgacctccctagcaaactggggcacagatgatactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagccac
aattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaag
gtcgcgtattcaggcgctttttagactggtcgtaatgaacgaagcttaaagaggagaaagatcatatgcatcatcatca
tcatcatacgccaaccattgaacttatttgtggccatcgctccattcgccatttcactgatgaacccatttccgaagcg
cagcgtgaggcgattattaacagcgcccgtgcgacgtccagttccagttttttgcagtgcagtagcattattcgcatta
ccgacaaagcgttacgtgaagaactggtgacgctgaccggcgggcaaaaacacgtagcgcaagcggcggagttctgggt
gttctgtgccgactttaaccgccatttacaaatctgtccggatgctcagctcggcctggcggaacaactgttgctcggt
gtcgttgatacggcaatgatggcgcagaatgcattaatcgcagcggaatcgctgggattgggcggggtatatatcggcg
gcctgcgcaataatattgaagcggtgacgaaactgcttaaattaccgcagcatgttctgccgctgtttgggctgtgcct
tggctggcctgcggataatccggatcttaagccgcgtttaccggcctccattttggtgcatgaaaacagctatcaaccg
ctggataaaggcgcactggcgcagtatgacgagcaactggcggaatattacctcacccgtggcagcaataatcgccggg
atacctggagcgatcatatccgccgaacaatcattaaagaaagccgcccatttattctggattatttgcacaaacaggg
ttgggcgacgcgctaactaagtaaaaagaggagaaagatcatatgcatcatcatcatcatcatgatatcatttctgtcg
ccttaaagcgtcattccactaaggcatttgatgccagcaaaaaacttaccccggaacaggccgagcagatcaaaacgct
actgcaatacagcccatccagcaccaactcccagccgtggcattttattgttgccagcacggaagaaggtaaagcgcgt
gttgccaaatccgctgccggtaattacgtgttcaacgagcgtaaaatgcttgatgcctcgcacgtcgtggtgttctgtg
caaaaaccgcgatggacgatgtctggctgaagctggttgttgaccaggaagatgccgatggccgctttgccacgccgga
agcgaaagccgcgaacgataaaggtcgcaagttcttcgctgatatgcaccgtaaggatctgcatgatgatgcagagtgg
atggcaaaacaggtttatctcaacgtcggtaacttcctgctcggcgtggcggctctgggtctggacgcggtacccatcg
aaggttttgacgccgccatcctcgatgcagaatttggtctgaaagagaaaggctacaccagtctggtggttgttccggt
aggtcatcacagcgttgaagattttaacgctacgctgccgaaatctcgtctgccgcaaaacatcaccttaaccgaagtg
taactaagtaaaaagaggagaaagatcatatgtcatctgaaaaactgtattccccactgaaagtgggcgcgatcacggc
ggcaaaccgtatttttatggcaccgctgacgcgtctgcgcagtattgaaccgggtgacattcctaccccgttgatggcg
gaatactatcgccaacgtgccagtgccggtttgattattagtgaagccacgcaaatttctgcccaggcaaaaggatatg
caggtgcgcctggcatccatagtccggagcaaattgccgcatggaaaaaaatcaccgctggcgttcatgctgaaaatgg
tcatatggccgtgcaactgtggcacaccggacgcatttctcacgccagcctgcaacctggcggtcaggccccggtagcg
ccttcagcacttagcgcgggaacacgtacttctctgcgcgatgaaaatggtcaggcgatccgtgttgaaacatccatgc
cgcgtgcgcttgaactggaagagattccaggtatcgtcaatgatttccgtcaggccattgctaacgcgcgtgaagccgg
ttttgatctggtagagctccactctgctcacggttatttgctgcatcagttcctttctccttcttcaaaccatcgtacc
gatcagtacggcggcagcgtggaaaatcgcgcacgtttggtactggaagtggtcgatgccgggattgaagaatggggtg
ccgatcgcattggcattcgcgtttcaccaatcggtactttccagaacacagataacggcccgaatgaagaagccgatgc
actgtatctgattgaacaactgggtaaacgcggcattgcttatctgcatatgtcagaaccagattgggcggggggtgaa
ccgtatactgatgcgttccgcgaaaaagtacgcgcccgtttccacggtccgattatcggcgcaggtgcatacacagtag
aaaaagctgaaacgctgatcggcaaagggttaattgatgcggtggcatttggtcgtgactggattgcgaacccggatct
ggtcgcccgcttgcagcgcaaagctgagcttaacccacagcgtgccgaaagtttctacggtggcggcgcggaaggctat
accgattacccgacgttgcatcatcatcatcatcattaactaagtaatccggcaaaaaagggcaaggtgtcaccaccct
gccctttttctttaaaaccgaaaaga