BBa_K1316008
type: ComponentDefinition
persistent identity: BBa K1316008
display id: BBa_K1316008
version: 1
was generated by: iGEM to SBOL conversion
description: ybiJ promoter coupled to mKate2 reporter gene and Rhamnose promoter coupled to N-genes
created: 2014-09-12T11:00:00Z
modified: 2015-05-08T01:09:50Z
mutable provenance: Assembly of a part from Belkin laboratory (Belkin et al. Appl Microbiol Biotechnol (2014) 98:885???895) with a "de novo" synthesised part
owned by: james
owned by: myers
mutable description: Promoter of the ybiJ gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli. nfsA, nfsB and nemA (refered here as N-genes) are the genes for NfsA, NfsB and NEM reductases, which presumably play a role in 2,4-DNT and 2,4,6-TNT metabolism. These genes are regulated by the inducible Rhamnose promoter This construct is, then, designed to be able to detect and quantify the presence of compunds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. The presence of the N-genes aims to enhance this response.
discontinued: false
dominant: false
experience: experience/None
group u list: _1691_
m user id: 0
owner id: 19967
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The Rhamnose promoter was used to inducibly regulate the expression of the N-genes A double transcriptional terminator was placed between mKate2 and the N-genes in orther to avoid unfinished transcripts of the N-genes, as well as to avoid expression of these genes without the presence of rhamnose A strong RBS was placed in front of each gene. An "all frame STOP" region was placed after each N-gene. nfsA and nfsB have a 6xHis tag at the N-terminus; nemA has a 6xHis tag at the C-terminus. This way, the proteins can be purified to check if they are successfully expressed
star: false
creator: Joan Cortada Garcia
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_K1316006
component: BBa_K1316005
sequence annotation: BBa_K1316005
sequence annotation: BBa_K1316006
sequence: https://synbiohub.org/public/igem/BBa_K1316008_sequence/1

BBa_K1316006
type: Component
persistent identity: component2383045
display id: component2383045
version: 1
top level: BBa_K1316008
definition: BBa_K1316006
access: public

BBa_K1316005
type: Component
persistent identity: component2383027
display id: component2383027
version: 1
top level: BBa_K1316008
definition: BBa_K1316005
access: public

BBa_K1316005
type: SequenceAnnotation
persistent identity: annotation2383027
display id: annotation2383027
version: 1
top level: BBa_K1316008
location: https://synbiohub.org/public/igem/BBa_K1316008/annotation2383027/range2383027/1
component: BBa_K1316005

https://synbiohub.org/public/igem/BBa_K1316008/annotation2383027/range2383027/1
type: Range
persistent identity: range2383027
display id: range2383027
version: 1
top level: BBa_K1316008
direction: direction/reverse
start: 1
end: 1252
orientation: inline

BBa_K1316006
type: SequenceAnnotation
persistent identity: annotation2383045
display id: annotation2383045
version: 1
top level: BBa_K1316008
location: https://synbiohub.org/public/igem/BBa_K1316008/annotation2383045/range2383045/1
component: BBa_K1316006

https://synbiohub.org/public/igem/BBa_K1316008/annotation2383045/range2383045/1
type: Range
persistent identity: range2383045
display id: range2383045
version: 1
top level: BBa_K1316008
direction: direction/reverse
start: 1261
end: 4055
orientation: inline

BBa_K1316005
type: ComponentDefinition
persistent identity: BBa K1316005
display id: BBa_K1316005
version: 1
was generated by: iGEM to SBOL conversion
description: ybiJ promoter coupled to mKate2 reporter gene
created: 2014-08-23T11:00:00Z
modified: 2015-05-08T01:09:50Z
mutable provenance: Combination of BBa_K1316004 with BBa_K1316001
owned by: james
owned by: myers
mutable description: Promoter of the ybiJ gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli. This construct is, then, designed to be able to detect and quantify the presence of compunds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.
discontinued: false
dominant: false
group u list: _1691_
m user id: 0
owner id: 19967
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence was positively checked for the absence of illegal restriction sites. It is, therefore, biobrick-compatible
star: false
creator: Joan Cortada Garcia
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_K1316001
component: BBa_K1316004
sequence annotation: BBa_K1316004
sequence annotation: BBa_K1316001
sequence: https://synbiohub.org/public/igem/BBa_K1316005_sequence/1

BBa_K1316001
type: Component
persistent identity: component2381343
display id: component2381343
version: 1
top level: BBa_K1316005
definition: BBa_K1316001
access: public

BBa_K1316004
type: Component
persistent identity: component2381329
display id: component2381329
version: 1
top level: BBa_K1316005
definition: BBa_K1316004
access: public

BBa_K1316004
type: SequenceAnnotation
persistent identity: annotation2381329
display id: annotation2381329
version: 1
top level: BBa_K1316005
location: https://synbiohub.org/public/igem/BBa_K1316005/annotation2381329/range2381329/1
component: BBa_K1316004

https://synbiohub.org/public/igem/BBa_K1316005/annotation2381329/range2381329/1
type: Range
persistent identity: range2381329
display id: range2381329
version: 1
top level: BBa_K1316005
direction: direction/reverse
start: 1
end: 424
orientation: inline

BBa_K1316001
type: SequenceAnnotation
persistent identity: annotation2381343
display id: annotation2381343
version: 1
top level: BBa_K1316005
location: https://synbiohub.org/public/igem/BBa_K1316005/annotation2381343/range2381343/1
component: BBa_K1316001

https://synbiohub.org/public/igem/BBa_K1316005/annotation2381343/range2381343/1
type: Range
persistent identity: range2381343
display id: range2381343
version: 1
top level: BBa_K1316005
direction: direction/reverse
start: 433
end: 1252
orientation: inline

BBa_K1316006
type: ComponentDefinition
persistent identity: BBa K1316006
display id: BBa_K1316006
version: 1
was generated by: iGEM to SBOL conversion
description: Rhamnose promoter coupled to N-genes
created: 2014-09-11T11:00:00Z
modified: 2015-05-08T01:09:50Z
mutable provenance: "De novo" synthesis
owned by: james
owned by: myers
mutable description: nfsA, nfsB and nemA (refered here as N-genes) are the genes for NfsA, NfsB and NEM reductases, which presumably play a role in 2,4-DNT and 2,4,6-TNT metabolism. These genes are regulated by the inducible Rhamnose promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _1691_
m user id: 0
owner id: 19967
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The Rhamnose promoter was used to inducibly regulate the expression of the N-genes A strong RBS was placed in front of each gene An "all frame STOP" region was placed after each gene nfsA and nfsB have a 6xHis tag at the N-terminus; nemA has a 6xHis tag at the C-terminus. This way, the proteins can be purified to check if they are successfully expressed
star: false
creator: Joan Cortada Garcia
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Rhamnose promoter
sequence annotation: RBS B0034
sequence annotation: start
sequence annotation: nfsA
sequence annotation: 6xHis tag
sequence annotation: stop
sequence annotation: nfsB
sequence annotation: nemA
sequence annotation: His terminator
sequence: https://synbiohub.org/public/igem/BBa_K1316006_sequence/1

Rhamnose promoter
type: SequenceAnnotation
persistent identity: annotation2382943
display id: annotation2382943
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382943/range2382943/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382943/range2382943/1
type: Range
persistent identity: range2382943
display id: range2382943
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 1
end: 122
orientation: inline

RBS B0034
type: SequenceAnnotation
persistent identity: annotation2382944
display id: annotation2382944
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382944/range2382944/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382944/range2382944/1
type: Range
persistent identity: range2382944
display id: range2382944
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 129
end: 140
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2382958
display id: annotation2382958
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382958/range2382958/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382958/range2382958/1
type: Range
persistent identity: range2382958
display id: range2382958
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 149
end: 152
orientation: inline

nfsA
type: SequenceAnnotation
persistent identity: annotation2382945
display id: annotation2382945
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382945/range2382945/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382945/range2382945/1
type: Range
persistent identity: range2382945
display id: range2382945
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 149
end: 887
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation2382946
display id: annotation2382946
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382946/range2382946/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382946/range2382946/1
type: Range
persistent identity: range2382946
display id: range2382946
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 152
end: 170
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2382947
display id: annotation2382947
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382947/range2382947/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382947/range2382947/1
type: Range
persistent identity: range2382947
display id: range2382947
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 888
end: 891
orientation: inline

RBS B0034
type: SequenceAnnotation
persistent identity: annotation2382948
display id: annotation2382948
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382948/range2382948/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382948/range2382948/1
type: Range
persistent identity: range2382948
display id: range2382948
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 897
end: 909
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2382959
display id: annotation2382959
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382959/range2382959/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382959/range2382959/1
type: Range
persistent identity: range2382959
display id: range2382959
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 916
end: 918
orientation: inline

nfsB
type: SequenceAnnotation
persistent identity: annotation2382949
display id: annotation2382949
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382949/range2382949/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382949/range2382949/1
type: Range
persistent identity: range2382949
display id: range2382949
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 916
end: 1585
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation2382952
display id: annotation2382952
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382952/range2382952/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382952/range2382952/1
type: Range
persistent identity: range2382952
display id: range2382952
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 919
end: 937
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2382950
display id: annotation2382950
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382950/range2382950/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382950/range2382950/1
type: Range
persistent identity: range2382950
display id: range2382950
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 1585
end: 1588
orientation: inline

RBS B0034
type: SequenceAnnotation
persistent identity: annotation2382953
display id: annotation2382953
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382953/range2382953/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382953/range2382953/1
type: Range
persistent identity: range2382953
display id: range2382953
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 1600
end: 1612
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2382960
display id: annotation2382960
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382960/range2382960/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382960/range2382960/1
type: Range
persistent identity: range2382960
display id: range2382960
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 1619
end: 1621
orientation: inline

nemA
type: SequenceAnnotation
persistent identity: annotation2382954
display id: annotation2382954
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382954/range2382954/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382954/range2382954/1
type: Range
persistent identity: range2382954
display id: range2382954
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 1619
end: 2732
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation2382955
display id: annotation2382955
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382955/range2382955/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382955/range2382955/1
type: Range
persistent identity: range2382955
display id: range2382955
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 2714
end: 2732
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2382956
display id: annotation2382956
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382956/range2382956/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382956/range2382956/1
type: Range
persistent identity: range2382956
display id: range2382956
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 2733
end: 2735
orientation: inline

His terminator
type: SequenceAnnotation
persistent identity: annotation2382957
display id: annotation2382957
version: 1
top level: BBa_K1316006
location: https://synbiohub.org/public/igem/BBa_K1316006/annotation2382957/range2382957/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K1316006/annotation2382957/range2382957/1
type: Range
persistent identity: range2382957
display id: range2382957
version: 1
top level: BBa_K1316006
direction: direction/forward
start: 2737
end: 2795
orientation: inline

BBa_K1316001
type: ComponentDefinition
persistent identity: BBa K1316001
display id: BBa_K1316001
version: 1
was generated by: iGEM to SBOL conversion
description: mKate2 protein with double transcription terminator
created: 2014-08-23T11:00:00Z
modified: 2015-05-08T01:09:50Z
mutable provenance: Combination of BBa_K1316000 with BBa_K823017
owned by: james
owned by: myers
mutable description: mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli. BBa_K823017 Double Terminator (B0012-B0011)
discontinued: false
dominant: false
group u list: _1691_
m user id: 0
owner id: 19967
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The sequence was positively checked for the absence of illegal restriction sites. It is, therefore, biobrick-compatible
star: false
creator: Joan Cortada Garcia
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K823017
component: BBa_K1316000
sequence annotation: BBa_K1316000
sequence annotation: BBa_K823017
sequence: https://synbiohub.org/public/igem/BBa_K1316001_sequence/1

BBa_K823017
type: Component
persistent identity: component2381310
display id: component2381310
version: 1
top level: BBa_K1316001
definition: BBa_K823017
access: public

BBa_K1316000
type: Component
persistent identity: component2381302
display id: component2381302
version: 1
top level: BBa_K1316001
definition: BBa_K1316000
access: public

BBa_K1316000
type: SequenceAnnotation
persistent identity: annotation2381302
display id: annotation2381302
version: 1
top level: BBa_K1316001
location: https://synbiohub.org/public/igem/BBa_K1316001/annotation2381302/range2381302/1
component: BBa_K1316000

https://synbiohub.org/public/igem/BBa_K1316001/annotation2381302/range2381302/1
type: Range
persistent identity: range2381302
display id: range2381302
version: 1
top level: BBa_K1316001
direction: direction/reverse
start: 1
end: 717
orientation: inline

BBa_K823017
type: SequenceAnnotation
persistent identity: annotation2381310
display id: annotation2381310
version: 1
top level: BBa_K1316001
location: https://synbiohub.org/public/igem/BBa_K1316001/annotation2381310/range2381310/1
component: BBa_K823017

https://synbiohub.org/public/igem/BBa_K1316001/annotation2381310/range2381310/1
type: Range
persistent identity: range2381310
display id: range2381310
version: 1
top level: BBa_K1316001
direction: direction/reverse
start: 726
end: 820
orientation: inline

BBa_K1316004
type: ComponentDefinition
persistent identity: BBa K1316004
display id: BBa_K1316004
version: 1
was generated by: iGEM to SBOL conversion
description: ybiJ promoter
created: 2014-08-23T11:00:00Z
modified: 2015-05-08T01:09:50Z
mutable provenance: Belkin laboratory (Belkin et al. Appl Microbiol Biotechnol (2014) 98:885???895)
owned by: james
owned by: myers
mutable description: Promoter of the ybiJ gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.
discontinued: false
dominant: false
group u list: _1691_
m user id: 0
owner id: 19967
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The sequence was positively checked for the absence of illegal restriction sites. It is, therefore, biobrick-compatible
star: false
creator: Joan Cortada Garcia
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ybiJ promoter
sequence: https://synbiohub.org/public/igem/BBa_K1316004_sequence/1

ybiJ promoter
type: SequenceAnnotation
persistent identity: annotation2381327
display id: annotation2381327
version: 1
top level: BBa_K1316004
location: https://synbiohub.org/public/igem/BBa_K1316004/annotation2381327/range2381327/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1316004/annotation2381327/range2381327/1
type: Range
persistent identity: range2381327
display id: range2381327
version: 1
top level: BBa_K1316004
direction: direction/forward
start: 1
end: 408
orientation: inline

BBa_K1316000
type: ComponentDefinition
persistent identity: BBa K1316000
display id: BBa_K1316000
version: 1
was generated by: iGEM to SBOL conversion
description: mKate2 protein
created: 2014-08-23T11:00:00Z
modified: 2015-05-08T01:09:50Z
mutable provenance: pmKate2-N vector (evrogen)
owned by: james
owned by: myers
mutable description: mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli.
discontinued: false
dominant: false
group u list: _1691_
m user id: 0
owner id: 19967
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The sequence was positively checked for the absence of illegal restriction sites. It is, therefore, biobrick-compatible
star: false
creator: Joan Cortada Garcia
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: B0034 RBS
sequence annotation: start codon
sequence annotation: mKate2
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1316000_sequence/1

B0034 RBS
type: SequenceAnnotation
persistent identity: annotation2381284
display id: annotation2381284
version: 1
top level: BBa_K1316000
location: https://synbiohub.org/public/igem/BBa_K1316000/annotation2381284/range2381284/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1316000/annotation2381284/range2381284/1
type: Range
persistent identity: range2381284
display id: range2381284
version: 1
top level: BBa_K1316000
direction: direction/forward
start: 1
end: 12
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2381269
display id: annotation2381269
version: 1
top level: BBa_K1316000
location: https://synbiohub.org/public/igem/BBa_K1316000/annotation2381269/range2381269/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1316000/annotation2381269/range2381269/1
type: Range
persistent identity: range2381269
display id: range2381269
version: 1
top level: BBa_K1316000
direction: direction/forward
start: 19
end: 21
orientation: inline

mKate2
type: SequenceAnnotation
persistent identity: annotation2381268
display id: annotation2381268
version: 1
top level: BBa_K1316000
location: https://synbiohub.org/public/igem/BBa_K1316000/annotation2381268/range2381268/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1316000/annotation2381268/range2381268/1
type: Range
persistent identity: range2381268
display id: range2381268
version: 1
top level: BBa_K1316000
direction: direction/forward
start: 19
end: 717
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2381270
display id: annotation2381270
version: 1
top level: BBa_K1316000
location: https://synbiohub.org/public/igem/BBa_K1316000/annotation2381270/range2381270/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1316000/annotation2381270/range2381270/1
type: Range
persistent identity: range2381270
display id: range2381270
version: 1
top level: BBa_K1316000
direction: direction/forward
start: 715
end: 717
orientation: inline

BBa_K823017
type: ComponentDefinition
persistent identity: BBa K823017
display id: BBa_K823017
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2012-09-10T11:00:00Z
modified: 2015-05-08T01:13:30Z
mutable provenance: Part:BBa_B0014
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is a copy of the Part:B0014. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This is a copy of the Part:B0014. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
star: false
creator: Jara Radeck
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0014
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K823017_sequence/1

BBa_B0014
type: Component
persistent identity: component2182657
display id: component2182657
version: 1
top level: BBa_K823017
definition: BBa_B0014
access: public

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2182657
display id: annotation2182657
version: 1
top level: BBa_K823017
location: https://synbiohub.org/public/igem/BBa_K823017/annotation2182657/range2182657/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K823017/annotation2182657/range2182657/1
type: Range
persistent identity: range2182657
display id: range2182657
version: 1
top level: BBa_K823017
direction: direction/reverse
start: 1
end: 95
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K1316008_sequence/1
type: Sequence
persistent identity: BBa K1316008 sequence
display id: BBa_K1316008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatgcggtacccgcagaggttcgaaagacagccagttacgttaactacaactggcgagatgcatagcgagtataatttgtattttgcgtgtcattccgtgcctttaacgctgctgtgactgccacgcggtataaaacaagttcataagtacaacaaataaatggtttatcagtaggttagatatcaatcaatttatttgaacaaggcggtcaattctcttcgattttatctctcgtaaaaaaacgtgatactcatcacatcgacgaaacaacgtcacttatacaaaaatcacctgcgagagattaattatgaaaactatcaatactgttgttgctgctatggctctttcaactctgtcatttggcgtgttcgcggcggaaccggtaacggcatcccaggcacagaaccgatagacatatgctatactagagaaagaggagaaatataccatggtgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacagatgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaacgaagcttaaagaggagaaagatcatatgcatcatcatcatcatcatacgccaaccattgaacttatttgtggccatcgctccattcgccatttcactgatgaacccatttccgaagcgcagcgtgaggcgattattaacagcgcccgtgcgacgtccagttccagttttttgcagtgcagtagcattattcgcattaccgacaaagcgttacgtgaagaactggtgacgctgaccggcgggcaaaaacacgtagcgcaagcggcggagttctgggtgttctgtgccgactttaaccgccatttacaaatctgtccggatgctcagctcggcctggcggaacaactgttgctcggtgtcgttgatacggcaatgatggcgcagaatgcattaatcgcagcggaatcgctgggattgggcggggtatatatcggcggcctgcgcaataatattgaagcggtgacgaaactgcttaaattaccgcagcatgttctgccgctgtttgggctgtgccttggctggcctgcggataatccggatcttaagccgcgtttaccggcctccattttggtgcatgaaaacagctatcaaccgctggataaaggcgcactggcgcagt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1316005_sequence/1
type: Sequence
persistent identity: BBa K1316005 sequence
display id: BBa_K1316005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatgcggtacccgcagaggttcgaaagacagccagttacgttaactacaactggcgagatgcatagcgagtataatttgtattttgcgtgtcattccgtgcctttaacgctgctgtgactgccacgcggtataaaacaagttcataagtacaacaaataaatggtttatcagtaggttagatatcaatcaatttatttgaacaaggcggtcaattctcttcgattttatctctcgtaaaaaaacgtgatactcatcacatcgacgaaacaacgtcacttatacaaaaatcacctgcgagagattaattatgaaaactatcaatactgttgttgctgctatggctctttcaactctgtcatttggcgtgttcgcggcggaaccggtaacggcatcccaggcacagaaccgatagacatatgctatactagagaaagaggagaaatataccatggtgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacagatgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1316006_sequence/1
type: Sequence
persistent identity: BBa K1316006 sequence
display id: BBa_K1316006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaacgaagcttaaagaggagaaagatcatatgcatcatcatcatcatcatacgccaaccattgaacttatttgtggccatcgctccattcgccatttcactgatgaacccatttccgaagcgcagcgtgaggcgattattaacagcgcccgtgcgacgtccagttccagttttttgcagtgcagtagcattattcgcattaccgacaaagcgttacgtgaagaactggtgacgctgaccggcgggcaaaaacacgtagcgcaagcggcggagttctgggtgttctgtgccgactttaaccgccatttacaaatctgtccggatgctcagctcggcctggcggaacaactgttgctcggtgtcgttgatacggcaatgatggcgcagaatgcattaatcgcagcggaatcgctgggattgggcggggtatatatcggcggcctgcgcaataatattgaagcggtgacgaaactgcttaaattaccgcagcatgttctgccgctgtttgggctgtgccttggctggcctgcggataatccggatcttaagccgcgtttaccggcctccattttggtgcatgaaaacagctatcaaccgctggataaaggcgcactggcgcagtatgacgagcaactggcggaatattacctcacccgtggcagcaataatcgccgggatacctggagcgatcatatccgccgaacaatcattaaagaaagccgcccatttattctggattatttgcacaaacagggttgggcgacgcgctaactaagtaaaaagaggagaaagatcatatgcatcatcatcatcatcatgatatcatttctgtcgccttaaagcgtcattccactaaggcatttgatgccagcaaaaaacttaccccggaacaggccgagcagatcaaaacgctactgcaatacagcccatccagcaccaactcccagccgtggcattttattgttgccagcacggaagaaggtaaagcgcgtgttgccaaatccgctgccggtaattacgtgttcaacgagcgtaaaatgcttgatgcctcgcacgtcgtggtgttctgtgcaaaaaccgcgatggacgatgtctggctgaagctggttgttgaccaggaagatgccgatggccgctttgccacgccggaagcgaaagccgcgaacgataaaggtcgcaagttcttcgctgatatgcaccgtaaggatctgcatgatgatgcagagtggatggcaaaacaggtttatctcaacgtcggtaacttcctgctcggcgtggcggctctgggtctggacgcggtacccatcgaaggttttgacgccgccatcctcgatgcagaatttggtctgaaagagaaaggctacaccagtctggtggttgttccggtaggtcatcacagcgttgaagattttaacgctacgctgccgaaatctcgtctgccgcaaaacatcaccttaaccgaagtgtaactaagtaaaaagaggagaaagatcatatgtcatctgaaaaactgtattccccactgaaagtgggcgcgatcacggcggcaaaccgtatttttatggcaccgctgacgcgtctgcgcagtattgaaccgggtgacattcctaccccgttgatggcggaatactatcgccaacgtgccagtgccggtttgattattagtgaagccacgcaaatttctgcccaggcaaaaggatatgcaggtgcgcctggcatccatagtccggagcaaattgccgcatggaaaaaaatcaccgctggcgttcatgctgaaaatggtcatatggccgtgcaactgtggcacaccggacgcatttctcacgccagcctgcaacctggcggtcaggccccggtagcgccttcagcacttagcgcggga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1316001_sequence/1
type: Sequence
persistent identity: BBa K1316001 sequence
display id: BBa_K1316001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatataccatggtgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacagatgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1316004_sequence/1
type: Sequence
persistent identity: BBa K1316004 sequence
display id: BBa_K1316004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatgcggtacccgcagaggttcgaaagacagccagttacgttaactacaactggcgagatgcatagcgagtataatttgtattttgcgtgtcattccgtgcctttaacgctgctgtgactgccacgcggtataaaacaagttcataagtacaacaaataaatggtttatcagtaggttagatatcaatcaatttatttgaacaaggcggtcaattctcttcgattttatctctcgtaaaaaaacgtgatactcatcacatcgacgaaacaacgtcacttatacaaaaatcacctgcgagagattaattatgaaaactatcaatactgttgttgctgctatggctctttcaactctgtcatttggcgtgttcgcggcggaaccggtaacggcatcccaggcacagaaccgatagacatatgcta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1316000_sequence/1
type: Sequence
persistent identity: BBa K1316000 sequence
display id: BBa_K1316000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatataccatggtgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacagatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823017_sequence/1
type: Sequence
persistent identity: BBa K823017 sequence
display id: BBa_K823017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z