LOCUS       BBa_K1316009            2359 bp    DNA     linear       05-May-2025
DEFINITION  yqjF promoter coupled to mKate2 reporter gene and ybiJ promoter 
            coupled to mKate2 reporter gene
ACCESSION   BBa_K1316009
VERSION     BBa_K1316009.1  
FEATURES             Location/Qualifiers
     promoter        1..249
                     /label=yqjF promoter
     promoter        1..271
                     /label=BBa_K1316002
     CDS             298..996
                     /label=mKate2
     misc_feature    298..300
                     /label=start codon
     misc_feature    994..996
                     /label=stop codon
     RBS             280..291
                     /label=B0034 RBS
     misc_feature    280..996
                     /label=BBa_K1316000
     stem_loop       1012..1031
                     /label=T7 TE
     misc_feature    1038..1038
                     /label=stop
     polyA_site      1032..1045
                     /label=polya
     misc_feature    1005..1045
                     /label=BBa_B0012
     terminator      1005..1045
                     /label=BBa_B0012
     stem_loop       1066..1088
                     /label=stem_loop
     misc_feature    1054..1099
                     /label=BBa_B0011
     terminator      1054..1099
                     /label=BBa_B0011
     terminator      1005..1099
                     /label=BBa_B0014
     terminator      1005..1099
                     /label=BBa_K823017
     misc_feature    280..1099
                     /label=BBa_K1316001
     misc_feature    1..1099
                     /label=BBa_K1316003
     promoter        1108..1515
                     /label=ybiJ promoter
     promoter        1108..1531
                     /label=BBa_K1316004
     CDS             1558..2256
                     /label=mKate2
     misc_feature    1558..1560
                     /label=start codon
     misc_feature    2254..2256
                     /label=stop codon
     RBS             1540..1551
                     /label=B0034 RBS
     misc_feature    1540..2256
                     /label=BBa_K1316000
     stem_loop       2272..2291
                     /label=T7 TE
     misc_feature    2298..2298
                     /label=stop
     polyA_site      2292..2305
                     /label=polya
     misc_feature    2265..2305
                     /label=BBa_B0012
     terminator      2265..2305
                     /label=BBa_B0012
     stem_loop       2326..2348
                     /label=stem_loop
     misc_feature    2314..2359
                     /label=BBa_B0011
     terminator      2314..2359
                     /label=BBa_B0011
     terminator      2265..2359
                     /label=BBa_B0014
     terminator      2265..2359
                     /label=BBa_K823017
     misc_feature    1540..2359
                     /label=BBa_K1316001
     misc_feature    1108..2359
                     /label=BBa_K1316005
ORIGIN
        1 ggtggtaccc ggttttggcg tatggagcgc ctggcatctg gttaaaacga ccctcaagca
       61 gcaacagctt cgcggttaac ttccctctgg ccggagccat tccggcctta tccctcaaat
      121 tttttgaaga tctttgacaa ttttccttgc taacaatcat cattcaccac gtttatgatt
      181 ctctccatcg acagcaacga cgctaatacc gcgcctttgc acaaaaaaac aatcagcagc
      241 ctgagtggcc gagcatgcta gacatatgct atactagaga aagaggagaa atataccatg
      301 gtgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
      361 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      421 atgagaatca aggcggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
      481 agcttcatgt acggcagcaa aaccttcatc aaccacaccc agggcatccc cgacttcttt
      541 aagcagtcct tccccgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
      601 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
      661 atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
      721 gaggcctcca ccgagaccct gtaccccgct gacggcggcc tggaaggcag agccgacatg
      781 gccctgaagc tcgtgggcgg gggccacctg atctgcaact tgaagaccac atacagatcc
      841 aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacag aagactggaa
      901 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga
      961 tactgcgacc tccctagcaa actggggcac agatgatact agagtcacac tggctcacct
     1021 tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa
     1081 tccggctttt ttattatttt actagaggca tgcggtaccc gcagaggttc gaaagacagc
     1141 cagttacgtt aactacaact ggcgagatgc atagcgagta taatttgtat tttgcgtgtc
     1201 attccgtgcc tttaacgctg ctgtgactgc cacgcggtat aaaacaagtt cataagtaca
     1261 acaaataaat ggtttatcag taggttagat atcaatcaat ttatttgaac aaggcggtca
     1321 attctcttcg attttatctc tcgtaaaaaa acgtgatact catcacatcg acgaaacaac
     1381 gtcacttata caaaaatcac ctgcgagaga ttaattatga aaactatcaa tactgttgtt
     1441 gctgctatgg ctctttcaac tctgtcattt ggcgtgttcg cggcggaacc ggtaacggca
     1501 tcccaggcac agaaccgata gacatatgct atactagaga aagaggagaa atataccatg
     1561 gtgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
     1621 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     1681 atgagaatca aggcggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
     1741 agcttcatgt acggcagcaa aaccttcatc aaccacaccc agggcatccc cgacttcttt
     1801 aagcagtcct tccccgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     1861 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     1921 atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
     1981 gaggcctcca ccgagaccct gtaccccgct gacggcggcc tggaaggcag agccgacatg
     2041 gccctgaagc tcgtgggcgg gggccacctg atctgcaact tgaagaccac atacagatcc
     2101 aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacag aagactggaa
     2161 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga
     2221 tactgcgacc tccctagcaa actggggcac agatgatact agagtcacac tggctcacct
     2281 tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa
     2341 tccggctttt ttattattt
//