BBa_K1316000 1 BBa_K1316000 mKate2 protein 2014-08-23T11:00:00Z 2015-05-08T01:09:50Z pmKate2-N vector (evrogen) mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli. false false _1691_ 0 19967 9 Not in stock false The sequence was positively checked for the absence of illegal restriction sites. It is, therefore, biobrick-compatible false Joan Cortada Garcia annotation2381269 1 start codon range2381269 1 19 21 annotation2381284 1 B0034 RBS range2381284 1 1 12 annotation2381268 1 mKate2 range2381268 1 19 717 annotation2381270 1 stop codon range2381270 1 715 717 BBa_K823017 1 BBa_K823017 double terminator (B0012-B0011) 2012-09-10T11:00:00Z 2015-05-08T01:13:30Z Part:BBa_B0014 Released HQ 2013 This is a copy of the [[Part:B0014|Part:B0014]]. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3. false false _1081_ 0 11555 9 In stock false This is a copy of the [[Part:B0014|Part:B0014]]. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3. false Jara Radeck component2182657 1 BBa_B0014 annotation2182657 1 BBa_B0014 range2182657 1 1 95 BBa_K1316002 1 BBa_K1316002 yqjF promoter 2014-08-23T11:00:00Z 2015-05-08T01:09:50Z Belkin laboratory (Belkin et al. Appl Microbiol Biotechnol (2014) 98:885???895) Promoter of the yqjF gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. false false _1691_ 0 19967 9 Not in stock false The sequence was positively checked for the absence of illegal restriction sites. It is, therefore, biobrick-compatible false Joan Cortada Garcia annotation2381283 1 yqjF promoter range2381283 1 1 249 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K1316009 1 BBa_K1316009 yqjF promoter coupled to mKate2 reporter gene and ybiJ promoter coupled to mKate2 reporter gene 2014-09-12T11:00:00Z 2015-05-08T01:09:50Z Promoters from Belkin laboratory (Belkin et al. Appl Microbiol Biotechnol (2014) 98:885???895) Promoter of the yqjF and ybiJ genes, which are activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli. nfsA, nfsB and nemA (refered here as N-genes) are the genes for NfsA, NfsB and NEM reductases, which presumably play a role in 2,4-DNT and 2,4,6-TNT metabolism. These genes are regulated by the inducible Rhamnose promoter This construct is, then, designed to be able to detect and quantify the presence of compunds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. The presence of the N-genes aims to enhance this response. Having both promoters in the same plasmid aims to improve the cellular response in front of the inducing chemical compounds (2,4,6-TNT, 2,4-DNT and 1,3-DNB). false false _1691_ 0 19967 9 It's complicated false A double terminator was placed between both yqjF+mKate2 and ybiJ+mKate2 to avoid having interfering unfinished transcripts of the 2nd mKate2 copy false Joan Cortada Garcia component2383079 1 BBa_K1316005 component2383062 1 BBa_K1316003 annotation2383079 1 BBa_K1316005 range2383079 1 1108 2359 annotation2383062 1 BBa_K1316003 range2383062 1 1 1099 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939311 1 BBa_B0011 component939303 1 BBa_B0012 annotation939311 1 BBa_B0011 range939311 1 50 95 annotation939303 1 BBa_B0012 range939303 1 1 41 BBa_K1316005 1 BBa_K1316005 ybiJ promoter coupled to mKate2 reporter gene 2014-08-23T11:00:00Z 2015-05-08T01:09:50Z Combination of BBa_K1316004 with BBa_K1316001 Promoter of the ybiJ gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli. This construct is, then, designed to be able to detect and quantify the presence of compunds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. false false _1691_ 0 19967 9 It's complicated false The sequence was positively checked for the absence of illegal restriction sites. It is, therefore, biobrick-compatible false Joan Cortada Garcia component2381343 1 BBa_K1316001 component2381329 1 BBa_K1316004 annotation2381343 1 BBa_K1316001 range2381343 1 433 1252 annotation2381329 1 BBa_K1316004 range2381329 1 1 424 BBa_K1316004 1 BBa_K1316004 ybiJ promoter 2014-08-23T11:00:00Z 2015-05-08T01:09:50Z Belkin laboratory (Belkin et al. Appl Microbiol Biotechnol (2014) 98:885???895) Promoter of the ybiJ gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. false false _1691_ 0 19967 9 Not in stock false The sequence was positively checked for the absence of illegal restriction sites. It is, therefore, biobrick-compatible false Joan Cortada Garcia annotation2381327 1 ybiJ promoter range2381327 1 1 408 BBa_K1316001 1 BBa_K1316001 mKate2 protein with double transcription terminator 2014-08-23T11:00:00Z 2015-05-08T01:09:50Z Combination of BBa_K1316000 with BBa_K823017 mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli. BBa_K823017 Double Terminator (B0012-B0011) false false _1691_ 0 19967 9 Not in stock false The sequence was positively checked for the absence of illegal restriction sites. It is, therefore, biobrick-compatible false Joan Cortada Garcia component2381310 1 BBa_K823017 component2381302 1 BBa_K1316000 annotation2381310 1 BBa_K823017 range2381310 1 726 820 annotation2381302 1 BBa_K1316000 range2381302 1 1 717 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_K1316003 1 BBa_K1316003 yqjF promoter coupled to mKate2 reporter gene 2014-08-23T11:00:00Z 2015-05-08T01:09:50Z Combination of K1316002 with K1316001 Promoter of the yqjF gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli. This construct is, then, designed to be able to detect and quantify the presence of compunds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. false false _1691_ 0 19967 9 It's complicated false The yqjF promoter carries 4 point mutations to improve its response in front of 2,4,6-TNT and 2,4-DNT false Joan Cortada Garcia component2381326 1 BBa_K1316001 component2381312 1 BBa_K1316002 annotation2381312 1 BBa_K1316002 range2381312 1 1 271 annotation2381326 1 BBa_K1316001 range2381326 1 280 1099 BBa_K1316001_sequence 1 aaagaggagaaatataccatggtgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacagatgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1316000_sequence 1 aaagaggagaaatataccatggtgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacagatga BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1316002_sequence 1 ggtggtacccggttttggcgtatggagcgcctggcatctggttaaaacgaccctcaagcagcaacagcttcgcggttaacttccctctggccggagccattccggccttatccctcaaattttttgaagatctttgacaattttccttgctaacaatcatcattcaccacgtttatgattctctccatcgacagcaacgacgctaataccgcgcctttgcacaaaaaaacaatcagcagcctgagtggccgagcatgctagacatatgcta BBa_K1316004_sequence 1 gcatgcggtacccgcagaggttcgaaagacagccagttacgttaactacaactggcgagatgcatagcgagtataatttgtattttgcgtgtcattccgtgcctttaacgctgctgtgactgccacgcggtataaaacaagttcataagtacaacaaataaatggtttatcagtaggttagatatcaatcaatttatttgaacaaggcggtcaattctcttcgattttatctctcgtaaaaaaacgtgatactcatcacatcgacgaaacaacgtcacttatacaaaaatcacctgcgagagattaattatgaaaactatcaatactgttgttgctgctatggctctttcaactctgtcatttggcgtgttcgcggcggaaccggtaacggcatcccaggcacagaaccgatagacatatgcta BBa_K823017_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1316005_sequence 1 gcatgcggtacccgcagaggttcgaaagacagccagttacgttaactacaactggcgagatgcatagcgagtataatttgtattttgcgtgtcattccgtgcctttaacgctgctgtgactgccacgcggtataaaacaagttcataagtacaacaaataaatggtttatcagtaggttagatatcaatcaatttatttgaacaaggcggtcaattctcttcgattttatctctcgtaaaaaaacgtgatactcatcacatcgacgaaacaacgtcacttatacaaaaatcacctgcgagagattaattatgaaaactatcaatactgttgttgctgctatggctctttcaactctgtcatttggcgtgttcgcggcggaaccggtaacggcatcccaggcacagaaccgatagacatatgctatactagagaaagaggagaaatataccatggtgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacagatgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1316003_sequence 1 ggtggtacccggttttggcgtatggagcgcctggcatctggttaaaacgaccctcaagcagcaacagcttcgcggttaacttccctctggccggagccattccggccttatccctcaaattttttgaagatctttgacaattttccttgctaacaatcatcattcaccacgtttatgattctctccatcgacagcaacgacgctaataccgcgcctttgcacaaaaaaacaatcagcagcctgagtggccgagcatgctagacatatgctatactagagaaagaggagaaatataccatggtgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacagatgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_K1316009_sequence 1 ggtggtacccggttttggcgtatggagcgcctggcatctggttaaaacgaccctcaagcagcaacagcttcgcggttaacttccctctggccggagccattccggccttatccctcaaattttttgaagatctttgacaattttccttgctaacaatcatcattcaccacgtttatgattctctccatcgacagcaacgacgctaataccgcgcctttgcacaaaaaaacaatcagcagcctgagtggccgagcatgctagacatatgctatactagagaaagaggagaaatataccatggtgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacagatgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagaggcatgcggtacccgcagaggttcgaaagacagccagttacgttaactacaactggcgagatgcatagcgagtataatttgtattttgcgtgtcattccgtgcctttaacgctgctgtgactgccacgcggtataaaacaagttcataagtacaacaaataaatggtttatcagtaggttagatatcaatcaatttatttgaacaaggcggtcaattctcttcgattttatctctcgtaaaaaaacgtgatactcatcacatcgacgaaacaacgtcacttatacaaaaatcacctgcgagagattaattatgaaaactatcaatactgttgttgctgctatggctctttcaactctgtcatttggcgtgttcgcggcggaaccggtaacggcatcccaggcacagaaccgatagacatatgctatactagagaaagaggagaaatataccatggtgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacagatgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z