##gff-version 3
##sequence-region BBa_K1316009 1 2359
BBa_K1316009	.	engineered_region	1	1099	.	+	0	ID=BBa_K1316003;Name=BBa_K1316003
BBa_K1316009	.	engineered_region	280	1099	.	+	0	ID=BBa_K1316001;Name=BBa_K1316001;Parent=BBa_K1316003
BBa_K1316009	.	terminator	1005	1099	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1316001
BBa_K1316009	.	terminator	1005	1099	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1316009	.	terminator	1054	1099	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1316009	.	stem_loop	1066	1088	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1316009	.	engineered_region	1054	1099	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1316009	.	terminator	1005	1045	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1316009	.	stem_loop	1012	1031	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1316009	.	stop_codon	1038	1038	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1316009	.	engineered_region	1005	1045	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1316009	.	polyA_site	1032	1045	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1316009	.	engineered_region	280	996	.	+	0	ID=BBa_K1316000;Name=BBa_K1316000;Parent=BBa_K1316001
BBa_K1316009	.	stop_codon	994	996	.	+	0	ID=annotation2381270;Name=stop codon;Parent=BBa_K1316000
BBa_K1316009	.	start_codon	298	300	.	+	0	ID=annotation2381269;Name=start codon;Parent=BBa_K1316000
BBa_K1316009	.	ribosome_entry_site	280	291	.	+	0	ID=annotation2381284;Name=B0034 RBS;Parent=BBa_K1316000
BBa_K1316009	.	CDS	298	996	.	+	0	ID=annotation2381268;Name=mKate2;Parent=BBa_K1316000
BBa_K1316009	.	promoter	1	271	.	+	0	ID=BBa_K1316002;Name=BBa_K1316002;Parent=BBa_K1316003
BBa_K1316009	.	promoter	1	249	.	+	0	ID=annotation2381283;Name=yqjF promoter;Parent=BBa_K1316002
BBa_K1316009	.	engineered_region	1108	2359	.	+	0	ID=BBa_K1316005;Name=BBa_K1316005
BBa_K1316009	.	promoter	1108	1531	.	+	0	ID=BBa_K1316004;Name=BBa_K1316004;Parent=BBa_K1316005
BBa_K1316009	.	promoter	1108	1515	.	+	0	ID=annotation2381327;Name=ybiJ promoter;Parent=BBa_K1316004
BBa_K1316009	.	engineered_region	1540	2359	.	+	0	ID=BBa_K1316001;Name=BBa_K1316001;Parent=BBa_K1316005
BBa_K1316009	.	terminator	2265	2359	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1316001
BBa_K1316009	.	terminator	2265	2359	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1316009	.	terminator	2314	2359	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1316009	.	stem_loop	2326	2348	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1316009	.	engineered_region	2314	2359	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1316009	.	terminator	2265	2305	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1316009	.	stem_loop	2272	2291	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1316009	.	stop_codon	2298	2298	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1316009	.	engineered_region	2265	2305	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1316009	.	polyA_site	2292	2305	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1316009	.	engineered_region	1540	2256	.	+	0	ID=BBa_K1316000;Name=BBa_K1316000;Parent=BBa_K1316001
BBa_K1316009	.	stop_codon	2254	2256	.	+	0	ID=annotation2381270;Name=stop codon;Parent=BBa_K1316000
BBa_K1316009	.	start_codon	1558	1560	.	+	0	ID=annotation2381269;Name=start codon;Parent=BBa_K1316000
BBa_K1316009	.	ribosome_entry_site	1540	1551	.	+	0	ID=annotation2381284;Name=B0034 RBS;Parent=BBa_K1316000
BBa_K1316009	.	CDS	1558	2256	.	+	0	ID=annotation2381268;Name=mKate2;Parent=BBa_K1316000
>BBa_K1316009
ggtggtacccggttttggcgtatggagcgcctggcatctggttaaaacgaccctcaagcagcaacagcttcgcggttaa
cttccctctggccggagccattccggccttatccctcaaattttttgaagatctttgacaattttccttgctaacaatc
atcattcaccacgtttatgattctctccatcgacagcaacgacgctaataccgcgcctttgcacaaaaaaacaatcagc
agcctgagtggccgagcatgctagacatatgctatactagagaaagaggagaaatataccatggtgagcgagctgatta
aggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaagg
caagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctg
gctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttcc
ccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctcca
ggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaaca
ctcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagc
tcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcc
cggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtg
gctgtggccagatactgcgacctccctagcaaactggggcacagatgatactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactaga
ggcatgcggtacccgcagaggttcgaaagacagccagttacgttaactacaactggcgagatgcatagcgagtataatt
tgtattttgcgtgtcattccgtgcctttaacgctgctgtgactgccacgcggtataaaacaagttcataagtacaacaa
ataaatggtttatcagtaggttagatatcaatcaatttatttgaacaaggcggtcaattctcttcgattttatctctcg
taaaaaaacgtgatactcatcacatcgacgaaacaacgtcacttatacaaaaatcacctgcgagagattaattatgaaa
actatcaatactgttgttgctgctatggctctttcaactctgtcatttggcgtgttcgcggcggaaccggtaacggcat
cccaggcacagaaccgatagacatatgctatactagagaaagaggagaaatataccatggtgagcgagctgattaagga
gaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaag
ccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggcta
ccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccga
gggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggac
ggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcg
gctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgt
gggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggc
gtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctg
tggccagatactgcgacctccctagcaaactggggcacagatgatactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt