BBa_K1316010 1 BBa_K1316010 pFAB640 2014-09-12T11:00:00Z 2015-05-08T01:09:50Z Caroline M. Ajo-Franklin laboratory (Goldbeck et al. ACS Synth. Biol. 2013, 2, 150−159) Best performing promoter to regulate the cytochrome c maturation (ccm) cluster generated by Goldbeck et al. false false _1691_ 0 19967 9 Not in stock false An NdeI restriction site was placed downstream the promoter to be able to easily change the promoter false Joan Cortada Garcia annotation2383080 1 pFAB640 promoter range2383080 1 25 61 BBa_K917006 1 BBa_K917006 E. coli cytochrome maturation gene cluster ccmA-H 2012-08-09T11:00:00Z 2015-05-08T01:13:46Z Escherichia coli JM109 chromosomal DNA This part consists of the native Escherichia coli cytochrome maturation gene cluster formed by 8 genes and their native RBSs: ccmA, ccmB, ccmC, ccmD, ccmE, ccmF, ccmG, ccmH. The function of this cluster is to control folding and transport of cytochromes. false false _1182_ 0 13804 9 In stock true Obtained by PCR amplification of E coli JM109 gene cluster false Jakub Krakowiak annotation2191514 1 Stop range2191514 1 6465 6467 annotation2191512 1 RBS range2191512 1 25 27 annotation2191513 1 Start codon range2191513 1 31 33 BBa_K1316011 1 BBa_K1316011 pFAB640 regulating the ccmAH cluster 2014-09-12T11:00:00Z 2015-05-08T01:09:50Z Caroline M. Ajo-Franklin laboratory (Goldbeck et al. ACS Synth. Biol. 2013, 2, 150−159) and Edinburgh 2012 iGEM team Cytochrome c maturation (ccm) cluster regulated by the best performing promoter generated by Goldbeck et al. regulating the false false _1691_ 0 19967 9 It's complicated true An NdeI restriction site was placed between the promoter and the ccm cluster to be able to easily exchange the promoter false Joan Cortada Garcia component2383110 1 BBa_K917006 component2383106 1 BBa_K1316010 annotation2383110 1 BBa_K917006 range2383110 1 102 6568 annotation2383106 1 BBa_K1316010 range2383106 1 1 93 BBa_K917006_sequence 1 gagtgccgtaactgtcacaacttcgagtatatggatacaaccgcccagaaatcggttgccgcgaagatgcatgaccaggcggtgaaagatgggcaaacctgtattgattgccataaagggatagcgcacaagctgcccgatatgcgtgaagtcgagccaggtttttaacagggttattgcgtgggtatgcttgaagccagagagttactttgtgagcgggatgaacgaaccttatttagtggcttgtcatttacgctgaacgcaggagagtgggtacaaatcaccggtagcaacggcgcggggaagacaacgcttctccgtttgctgacggggttgtctcgccctgacgcaggcgaggttctctggcaagggcagcccttgcatcaggtacgcgacagctaccatcaaaacctgttatggataggccatcagccggggatcaaaacccggctgacggcgttagaaaatctgcacttttatcatcgcgatggcgataccgcacaatgtctggaagccctggcgcaggccgggcttgccggattcgaagatattcctgtaaatcagctctcggccgggcaacaacgccgcgtcgctttagcgcgtctgtggctgacccgtgccacgttatggatcctcgacgagccttttaccgcgattgacgttaacggtgtcgatcgtctgacccagcgtatggcgcagcatacggagcagggggggattgtgattctgactacccaccagccgctcaacgttgctgaaagtaaaattcgccgcatttcactgacgcaaacgagggccgcatgatgttctggcgcattttccgtcttgagctgcgtgtagcgtttcgccatagcgccgaaatcgccaacccgctgtggttcttcctgattgtaattaccctttttccgctcagtatcggtccggagccgcaactgctggcgcgtattgcaccgggcattatctgggttgctgcgctgctttcatccttgctggcgctggaacgactgttccgtgacgatttgcaggacggcagtcttgaacaattgatgttgttgccgttacccttgcccgccgttgtgctggcgaaggtgatggcgcactggatggtaaccggtctgccgttactcatcctttcgccactggtagcaatgctactgggaatggatgtttatggctggcaagtgatggcgctgacgctgctgctgggaacgcctacgcttggctttctcggtgcaccgggcgtggcgctgacagtgggacttaagcgcggtggtgtgctgctcagcatactggtgttaccgctgactatcccattactcatctttgccaccgccgcgatggacgcggcttctatgcatttgcccgttgacgggtatctggcaattttaggcgcgttgctggcaggcaccgcgacattaagtccttttgcgacggcggcagcgttacgaatcagcattcaataacgctggattactgatttttgttcgagtctggtatcgaaactatgtggaaaacactgcatcaactggcgatcccaccacggctgtatcaaatctgtggctggtttataccgtggctggcaattgccagtgtggtcgtgcttaccgtcggctggatctggggattcggctttgctccggctgattatcagcagggaaatagctaccgcattatctacctgcatgtgcctgcggcgatctggtcgatgggcatttatgcatcaatggcagtggcagcgtttattggccttgtctggcagatgaaaatggccaacctggcggtggcggcgatggcccccattggtgccgtgtttacctttattgccctggttaccggctctgcatggggaaaaccgatgtggggcacctggtgggtatgggatgcacgtctgacttctgaactggtgctgctgtttttgtatgtgggtgtgattgccctgtggcacgccttcgacgaccgccgtctggcgggccgtgcggcaggtatcctggtgctgattggcgtggtgaatctgccgattattcattactccgtggagtggtggaacaccctgcatcagggatcaacgcggatgcagcaaagtatcgatccggcgatgcgttcgccgctgcgctggtcgatttttggcttcctgctcctgtctgccacgctgacgctgatgcggatgcgtaatttgattttgctgatggaaaaacgccgtccgtgggtgagtgaactgatactgaaaagaggccgtaaatgacccctgcatttgcttcctggaatgaatttttcgcaatgggcggttacgccttttttgtctggctggcggtggtgatgaccgttattccgctggtggttttggtcgtgcactcggtgatgcaacatcgcgcaattctgcgtggcgtggcgcaacagcgggcgcgtgaggcgcgtttacgtgctgcgcaacagcaggaggctgcatgaatattcgccgtaaaaaccgcttgtggattgcctgtgccgtgttggcagggctggcgctgactatcggtctggtgctatatgcgctgcgctcgaatatcgatctcttttatacgccgggggaaattctctacggcaagcgtgaaactcagcaaatgccggaagtcggtcagcgtctgcgcgttggcgggatggtgatgccgggtagtgtgcagcgcgatcccaattcgctgaaagtgaccttcaccatttacgatgctgaaggctcagtggatgtctcttacgaaggcattttgccggatctgttccgtgaagggcagggcgttgtggtgcagggcgaactggaaaaaggcaatcatatcctcgcgaaagaagtgctggcgaaacacgatgaaaactatacgccgccagaagttgagaaagcgatggaagctaaccaccgtcgcccggcgagtgtttataaggacccagcatcatgatgccagaaattggtaacggactgctgtgcctggcgctgggaattgcgctgctgctgtccgtgtatccgctatggggcgtggcgcgcggagatgcgcgcatgatggcgtcttcccgcttgtttgcctggctgctgtttatgtctgtggctggcgcatttctggtgctggtcaatgctttcgtggtcaacgacttcaccgtcacctatgttgccagcaactccaatacccagcttccggtgtggtatcgcgtggcggctacctggggcgcgcatgaaggctcgctactgctgtgggtgctgctgatgagcggctggacctttgcggtggcgatttttagtcagcgtattccgctggatattgtggcccgtgtactggcgataatggggatggtcagtgtcggctttttgctgttcattctctttacctctaacccgttctctcgcacgttgccgaacttcccgattgaaggtcgcgatcttaacccgctattgcaggatccggggctgatcttccatccgcctctgctttatatggggtacgtgggtttctcggtggcgtttgcttttgccattgcttctttgctgagcgggcgtctggacagcacttatgcgcgttttactcgtccgtggacgctggcagcgtggatcttcctgacgctcggcatcgtgctcggttccgcatgggcctattacgaactcggctggggtggctggtggttctgggatccggtagaaaacgcctcgtttatgccgtggctggtggggactgcgctgatgcactcactggcggtcactgaacaacgcgccagcttcaaagcgtggacattactgctggcaatcagtgccttctcgttgtgtctgttggggactttcctcgtgcgttccggcgtgctggtatcggtacacgcgtttgcgtctgatccggcgcgcggtatgtttatcctcgcctttatggtgctggtgattggcggttcgctgctgctgtttgccgcgcgtggacacaaagttcgctcacgcgtaaacaatgcgctgtggtcgcgggaatctttgctgttagcgaacaatgttttgctggtcgctgcgatgctggtggtgttgctggggacgctgctgccgttggtgcataagcaactgggactgggcagtatttcgattggcgaaccgttcttcaacaccatgtttacctggctgatggtgccgtttgcgctactgcttggtgtcggtcctctggtgcgctgggggcgggatcgcccgcgtaagatccgcaatttattgattatcgccttcatctctacgctggtgctgtcgctgctgttgccgtggctgttcgaaagcaaagttgtggcgatgacggtgctcggcctggcaatggcctgctggattgcggtgctggcaattgcggaagctgcgctacgtatttcacgcggcacgaaaaccaccttcagttattgggggatggtggcggctcaccttgggctggcagtgacaattgttggcattgcctttagccagaactatagcgttgagcgtgatgtgcgcatgaagtccggcgatagcgtcgatattcatgaatatcgcttcaccttccgtgatgtcaaagaggtgactggcccgaactggcgtggcggtgtggcgactatcggcgtaacgcgcgatggcaagccggaaacggtgctgtatgcggaaaaacgttattacaacactgccgggtcgatgatgaccgaagcggcaattgacggcggcatcacgcgtgacctgtacgcggccctcggtgaagagctggaaaacggcgcgtgggccgtgcgtctttactacaaaccatttgttcgctggatttgggcgggcgggctgatgatggcgttgggcggactgctgtgtctgtttgatcctcgctatcgtaagcgcgtgagtccgcaaaaaactgcgccggaggccgtatgaagcgcaaagtattgttaattccgttgattatcttcctggcgattgccgcggcgctgctgtggcagctggcgcgtaatgccgaaggggatgatccgaccaatctggaatcggcgctcattggcaagcctgtgccgaagtttcgtctcgaatcactggacaatccggggcagttttatcaggcggatgtgctgactcagggcaaaccagtactgcttaacgtctgggcgacctggtgtccgacctgccgtgcggaacatcaatatctgaatcagctttctgcgcagggcatccgcgtggtcggcatgaactataaagacgatcgccagaaggcaatcagctggctgaaagagctgggcaatccttacgcgctaagcctgtttgatggcgatggcatgttagggctggatctcggtgtctatggcgcgccagaaacgtttcttattgacggcaacggcatcattcgctatcgccatgcgggcgatctgaatcctcgcgtctgggaagaagagatcaagccgctgtgggagaaatacagtaaggaggccgcacaatgaggtttttattgggcgtgctgatgctgatgatctccggctcagcgctggcgaccatcgatgtgttgcagtttaaagatgaagcacaggaacaacagttccgtcagctcactgaagaactgcgctgcccgaaatgccagaacaacagcattgccgattccaactcgatgattgccaccgacctgcgtcagaaagtgtatgaactgatgcaggaaggtaaaagtaaaaaagagattgtcgattatatggtggcgcgttacggcaacttcgtcacttacgatccgccgttaacgccgctgaccgtgctgctgtgggtgctgccagtagtggctattggcattggcggttgggtcatatacgcccgctcgcggcgtcgggtacgcgtggtgccggaagcgtttcctgaacaaagcgtgccggaaggtaagcgtgccggatatgttgtttatctgccgggtattgtggtggcgttaattgtggctggcgtcagctactaccagactggcaattatcagcaggtgaaaatctggcagcaggccacggcacaggctccggcgttgctggacagggcgctggatccgaaagccgatccgctcaacgaagaagagatgtcgcgtcttgcgctggggatgcgtactcaactgcaaaaaaatccgggagatatagaaggctggattatgttgggccgcgttggcatggcgctgggtaacgccagtatcgccaccgatgcatacgccactgcgtatcgcctcgatccgaaaaacagtgatgctgcactgggatacgctgaagcgttgactcgttcatctgatcccaacgacaaccgcctcggcggtgaactgctacgtcagctggtgagaacggaccatagcaatatccgtgtgctaagcatgtatgcgtttaatgcctttgagcagcagcgatttggcgaagccgttgccgcgtgggagatgatgttgaaactcttacctgccaacgatactcgccgtgcggtgattgaacgtagtatcgcgcaggcgatgcaacatttgtcgccgcaggagagtaaataa BBa_K1316010_sequence 1 tgtaattctcatgtgctgcataactttgcggttaatcatccggctcgtataatgtgtggacttcatatgcagccaggtttttaacagggttat BBa_K1316011_sequence 1 tgtaattctcatgtgctgcataactttgcggttaatcatccggctcgtataatgtgtggacttcatatgcagccaggtttttaacagggttattactagaggagtgccgtaactgtcacaacttcgagtatatggatacaaccgcccagaaatcggttgccgcgaagatgcatgaccaggcggtgaaagatgggcaaacctgtattgattgccataaagggatagcgcacaagctgcccgatatgcgtgaagtcgagccaggtttttaacagggttattgcgtgggtatgcttgaagccagagagttactttgtgagcgggatgaacgaaccttatttagtggcttgtcatttacgctgaacgcaggagagtgggtacaaatcaccggtagcaacggcgcggggaagacaacgcttctccgtttgctgacggggttgtctcgccctgacgcaggcgaggttctctggcaagggcagcccttgcatcaggtacgcgacagctaccatcaaaacctgttatggataggccatcagccggggatcaaaacccggctgacggcgttagaaaatctgcacttttatcatcgcgatggcgataccgcacaatgtctggaagccctggcgcaggccgggcttgccggattcgaagatattcctgtaaatcagctctcggccgggcaacaacgccgcgtcgctttagcgcgtctgtggctgacccgtgccacgttatggatcctcgacgagccttttaccgcgattgacgttaacggtgtcgatcgtctgacccagcgtatggcgcagcatacggagcagggggggattgtgattctgactacccaccagccgctcaacgttgctgaaagtaaaattcgccgcatttcactgacgcaaacgagggccgcatgatgttctggcgcattttccgtcttgagctgcgtgtagcgtttcgccatagcgccgaaatcgccaacccgctgtggttcttcctgattgtaattaccctttttccgctcagtatcggtccggagccgcaactgctggcgcgtattgcaccgggcattatctgggttgctgcgctgctttcatccttgctggcgctggaacgactgttccgtgacgatttgcaggacggcagtcttgaacaattgatgttgttgccgttacccttgcccgccgttgtgctggcgaaggtgatggcgcactggatggtaaccggtctgccgttactcatcctttcgccactggtagcaatgctactgggaatggatgtttatggctggcaagtgatggcgctgacgctgctgctgggaacgcctacgcttggctttctcggtgcaccgggcgtggcgctgacagtgggacttaagcgcggtggtgtgctgctcagcatactggtgttaccgctgactatcccattactcatctttgccaccgccgcgatggacgcggcttctatgcatttgcccgttgacgggtatctggcaattttaggcgcgttgctggcaggcaccgcgacattaagtccttttgcgacggcggcagcgttacgaatcagcattcaataacgctggattactgatttttgttcgagtctggtatcgaaactatgtggaaaacactgcatcaactggcgatcccaccacggctgtatcaaatctgtggctggtttataccgtggctggcaattgccagtgtggtcgtgcttaccgtcggctggatctggggattcggctttgctccggctgattatcagcagggaaatagctaccgcattatctacctgcatgtgcctgcggcgatctggtcgatgggcatttatgcatcaatggcagtggcagcgtttattggccttgtctggcagatgaaaatggccaacctggcggtggcggcgatggcccccattggtgccgtgtttacctttattgccctggttaccggctctgcatggggaaaaccgatgtggggcacctggtgggtatgggatgcacgtctgacttctgaactggtgctgctgtttttgtatgtgggtgtgattgccctgtggcacgccttcgacgaccgccgtctggcgggccgtgcggcaggtatcctggtgctgattggcgtggtgaatctgccgattattcattactccgtggagtggtggaacaccctgcatcagggatcaacgcggatgcagcaaagtatcgatccggcgatgcgttcgccgctgcgctggtcgatttttggcttcctgctcctgtctgccacgctgacgctgatgcggatgcgtaatttgattttgctgatggaaaaacgccgtccgtgggtgagtgaactgatactgaaaagaggccgtaaatgacccctgcatttgcttcctggaatgaatttttcgcaatgggcggttacgccttttttgtctggctggcggtggtgatgaccgttattccgctggtggttttggtcgtgcactcggtgatgcaacatcgcgcaattctgcgtggcgtggcgcaacagcgggcgcgtgaggcgcgtttacgtgctgcgcaacagcaggaggctgcatgaatattcgccgtaaaaaccgcttgtggattgcctgtgccgtgttggcagggctggcgctgactatcggtctggtgctatatgcgctgcgctcgaatatcgatctcttttatacgccgggggaaattctctacggcaagcgtgaaactcagcaaatgccggaagtcggtcagcgtctgcgcgttggcgggatggtgatgccgggtagtgtgcagcgcgatcccaattcgctgaaagtgaccttcaccatttacgatgctgaaggctcagtggatgtctcttacgaaggcattttgccggatctgttccgtgaagggcagggcgttgtggtgcagggcgaactggaaaaaggcaatcatatcctcgcgaaagaagtgctggcgaaacacgatgaaaactatacgccgccagaagttgagaaagcgatggaagctaaccaccgtcgcccggcgagtgtttataaggacccagcatcatgatgccagaaattggtaacggactgctgtgcctggcgctgggaattgcgctgctgctgtccgtgtatccgctatggggcgtggcgcgcggagatgcgcgcatgatggcgtcttcccgcttgtttgcctggctgctgtttatgtctgtggctggcgcatttctggtgctggtcaatgctttcgtggtcaacgacttcaccgtcacctatgttgccagcaactccaatacccagcttccggtgtggtatcgcgtggcggctacctggggcgcgcatgaaggctcgctactgctgtgggtgctgctgatgagcggctggacctttgcggtggcgatttttagtcagcgtattccgctggatattgtggcccgtgtactggcgataatggggatggtcagtgtcggctttttgctgttcattctctttacctctaacccgttctctcgcacgttgccgaacttcccgattgaaggtcgcgatcttaacccgctattgcaggatccggggctgatcttccatccgcctctgctttatatggggtacgtgggtttctcggtggcgtttgcttttgccattgcttctttgctgagcgggcgtctggacagcacttatgcgcgttttactcgtccgtggacgctggcagcgtggatcttcctgacgctcggcatcgtgctcggttccgcatgggcctattacgaactcggctggggtggctggtggttctgggatccggtagaaaacgcctcgtttatgccgtggctggtggggactgcgctgatgcactcactggcggtcactgaacaacgcgccagcttcaaagcgtggacattactgctggcaatcagtgccttctcgttgtgtctgttggggactttcctcgtgcgttccggcgtgctggtatcggtacacgcgtttgcgtctgatccggcgcgcggtatgtttatcctcgcctttatggtgctggtgattggcggttcgctgctgctgtttgccgcgcgtggacacaaagttcgctcacgcgtaaacaatgcgctgtggtcgcgggaatctttgctgttagcgaacaatgttttgctggtcgctgcgatgctggtggtgttgctggggacgctgctgccgttggtgcataagcaactgggactgggcagtatttcgattggcgaaccgttcttcaacaccatgtttacctggctgatggtgccgtttgcgctactgcttggtgtcggtcctctggtgcgctgggggcgggatcgcccgcgtaagatccgcaatttattgattatcgccttcatctctacgctggtgctgtcgctgctgttgccgtggctgttcgaaagcaaagttgtggcgatgacggtgctcggcctggcaatggcctgctggattgcggtgctggcaattgcggaagctgcgctacgtatttcacgcggcacgaaaaccaccttcagttattgggggatggtggcggctcaccttgggctggcagtgacaattgttggcattgcctttagccagaactatagcgttgagcgtgatgtgcgcatgaagtccggcgatagcgtcgatattcatgaatatcgcttcaccttccgtgatgtcaaagaggtgactggcccgaactggcgtggcggtgtggcgactatcggcgtaacgcgcgatggcaagccggaaacggtgctgtatgcggaaaaacgttattacaacactgccgggtcgatgatgaccgaagcggcaattgacggcggcatcacgcgtgacctgtacgcggccctcggtgaagagctggaaaacggcgcgtgggccgtgcgtctttactacaaaccatttgttcgctggatttgggcgggcgggctgatgatggcgttgggcggactgctgtgtctgtttgatcctcgctatcgtaagcgcgtgagtccgcaaaaaactgcgccggaggccgtatgaagcgcaaagtattgttaattccgttgattatcttcctggcgattgccgcggcgctgctgtggcagctggcgcgtaatgccgaaggggatgatccgaccaatctggaatcggcgctcattggcaagcctgtgccgaagtttcgtctcgaatcactggacaatccggggcagttttatcaggcggatgtgctgactcagggcaaaccagtactgcttaacgtctgggcgacctggtgtccgacctgccgtgcggaacatcaatatctgaatcagctttctgcgcagggcatccgcgtggtcggcatgaactataaagacgatcgccagaaggcaatcagctggctgaaagagctgggcaatccttacgcgctaagcctgtttgatggcgatggcatgttagggctggatctcggtgtctatggcgcgccagaaacgtttcttattgacggcaacggcatcattcgctatcgccatgcgggcgatctgaatcctcgcgtctgggaagaagagatcaagccgctgtgggagaaatacagtaaggaggccgcacaatgaggtttttattgggcgtgctgatgctgatgatctccggctcagcgctggcgaccatcgatgtgttgcagtttaaagatgaagcacaggaacaacagttccgtcagctcactgaagaactgcgctgcccgaaatgccagaacaacagcattgccgattccaactcgatgattgccaccgacctgcgtcagaaagtgtatgaactgatgcaggaaggtaaaagtaaaaaagagattgtcgattatatggtggcgcgttacggcaacttcgtcacttacgatccgccgttaacgccgctgaccgtgctgctgtgggtgctgccagtagtggctattggcattggcggttgggtcatatacgcccgctcgcggcgtcgggtacgcgtggtgccggaagcgtttcctgaacaaagcgtgccggaaggtaagcgtgccggatatgttgtttatctgccgggtattgtggtggcgttaattgtggctggcgtcagctactaccagactggcaattatcagcaggtgaaaatctggcagcaggccacggcacaggctccggcgttgctggacagggcgctggatccgaaagccgatccgctcaacgaagaagagatgtcgcgtcttgcgctggggatgcgtactcaactgcaaaaaaatccgggagatatagaaggctggattatgttgggccgcgttggcatggcgctgggtaacgccagtatcgccaccgatgcatacgccactgcgtatcgcctcgatccgaaaaacagtgatgctgcactgggatacgctgaagcgttgactcgttcatctgatcccaacgacaaccgcctcggcggtgaactgctacgtcagctggtgagaacggaccatagcaatatccgtgtgctaagcatgtatgcgtttaatgcctttgagcagcagcgatttggcgaagccgttgccgcgtgggagatgatgttgaaactcttacctgccaacgatactcgccgtgcggtgattgaacgtagtatcgcgcaggcgatgcaacatttgtcgccgcaggagagtaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z