BBa_K1316010
1
BBa_K1316010
pFAB640
2014-09-12T11:00:00Z
2015-05-08T01:09:50Z
Caroline M. Ajo-Franklin laboratory (Goldbeck et al. ACS Synth. Biol. 2013, 2, 150−159)
Best performing promoter to regulate the cytochrome c maturation (ccm) cluster generated by Goldbeck et al.
false
false
_1691_
0
19967
9
Not in stock
false
An NdeI restriction site was placed downstream the promoter to be able to easily change the promoter
false
Joan Cortada Garcia
annotation2383080
1
pFAB640 promoter
range2383080
1
25
61
BBa_K917006
1
BBa_K917006
E. coli cytochrome maturation gene cluster ccmA-H
2012-08-09T11:00:00Z
2015-05-08T01:13:46Z
Escherichia coli JM109 chromosomal DNA
This part consists of the native Escherichia coli cytochrome maturation gene cluster formed by 8 genes and their native RBSs: ccmA, ccmB, ccmC, ccmD, ccmE, ccmF, ccmG, ccmH. The function of this cluster is to control folding and transport of cytochromes.
false
false
_1182_
0
13804
9
In stock
true
Obtained by PCR amplification of E coli JM109 gene cluster
false
Jakub Krakowiak
annotation2191514
1
Stop
range2191514
1
6465
6467
annotation2191512
1
RBS
range2191512
1
25
27
annotation2191513
1
Start codon
range2191513
1
31
33
BBa_K1316011
1
BBa_K1316011
pFAB640 regulating the ccmAH cluster
2014-09-12T11:00:00Z
2015-05-08T01:09:50Z
Caroline M. Ajo-Franklin laboratory (Goldbeck et al. ACS Synth. Biol. 2013, 2, 150−159) and Edinburgh 2012 iGEM team
Cytochrome c maturation (ccm) cluster regulated by the best performing promoter generated by Goldbeck et al. regulating the
false
false
_1691_
0
19967
9
It's complicated
true
An NdeI restriction site was placed between the promoter and the ccm cluster to be able to easily exchange the promoter
false
Joan Cortada Garcia
component2383110
1
BBa_K917006
component2383106
1
BBa_K1316010
annotation2383110
1
BBa_K917006
range2383110
1
102
6568
annotation2383106
1
BBa_K1316010
range2383106
1
1
93
BBa_K917006_sequence
1
gagtgccgtaactgtcacaacttcgagtatatggatacaaccgcccagaaatcggttgccgcgaagatgcatgaccaggcggtgaaagatgggcaaacctgtattgattgccataaagggatagcgcacaagctgcccgatatgcgtgaagtcgagccaggtttttaacagggttattgcgtgggtatgcttgaagccagagagttactttgtgagcgggatgaacgaaccttatttagtggcttgtcatttacgctgaacgcaggagagtgggtacaaatcaccggtagcaacggcgcggggaagacaacgcttctccgtttgctgacggggttgtctcgccctgacgcaggcgaggttctctggcaagggcagcccttgcatcaggtacgcgacagctaccatcaaaacctgttatggataggccatcagccggggatcaaaacccggctgacggcgttagaaaatctgcacttttatcatcgcgatggcgataccgcacaatgtctggaagccctggcgcaggccgggcttgccggattcgaagatattcctgtaaatcagctctcggccgggcaacaacgccgcgtcgctttagcgcgtctgtggctgacccgtgccacgttatggatcctcgacgagccttttaccgcgattgacgttaacggtgtcgatcgtctgacccagcgtatggcgcagcatacggagcagggggggattgtgattctgactacccaccagccgctcaacgttgctgaaagtaaaattcgccgcatttcactgacgcaaacgagggccgcatgatgttctggcgcattttccgtcttgagctgcgtgtagcgtttcgccatagcgccgaaatcgccaacccgctgtggttcttcctgattgtaattaccctttttccgctcagtatcggtccggagccgcaactgctggcgcgtattgcaccgggcattatctgggttgctgcgctgctttcatccttgctggcgctggaacgactgttccgtgacgatttgcaggacggcagtcttgaacaattgatgttgttgccgttacccttgcccgccgttgtgctggcgaaggtgatggcgcactggatggtaaccggtctgccgttactcatcctttcgccactggtagcaatgctactgggaatggatgtttatggctggcaagtgatggcgctgacgctgctgctgggaacgcctacgcttggctttctcggtgcaccgggcgtggcgctgacagtgggacttaagcgcggtggtgtgctgctcagcatactggtgttaccgctgactatcccattactcatctttgccaccgccgcgatggacgcggcttctatgcatttgcccgttgacgggtatctggcaattttaggcgcgttgctggcaggcaccgcgacattaagtccttttgcgacggcggcagcgttacgaatcagcattcaataacgctggattactgatttttgttcgagtctggtatcgaaactatgtggaaaacactgcatcaactggcgatcccaccacggctgtatcaaatctgtggctggtttataccgtggctggcaattgccagtgtggtcgtgcttaccgtcggctggatctggggattcggctttgctccggctgattatcagcagggaaatagctaccgcattatctacctgcatgtgcctgcggcgatctggtcgatgggcatttatgcatcaatggcagtggcagcgtttattggccttgtctggcagatgaaaatggccaacctggcggtggcggcgatggcccccattggtgccgtgtttacctttattgccctggttaccggctctgcatggggaaaaccgatgtggggcacctggtgggtatgggatgcacgtctgacttctgaactggtgctgctgtttttgtatgtgggtgtgattgccctgtggcacgccttcgacgaccgccgtctggcgggccgtgcggcaggtatcctggtgctgattggcgtggtgaatctgccgattattcattactccgtggagtggtggaacaccctgcatcagggatcaacgcggatgcagcaaagtatcgatccggcgatgcgttcgccgctgcgctggtcgatttttggcttcctgctcctgtctgccacgctgacgctgatgcggatgcgtaatttgattttgctgatggaaaaacgccgtccgtgggtgagtgaactgatactgaaaagaggccgtaaatgacccctgcatttgcttcctggaatgaatttttcgcaatgggcggttacgccttttttgtctggctggcggtggtgatgaccgttattccgctggtggttttggtcgtgcactcggtgatgcaacatcgcgcaattctgcgtggcgtggcgcaacagcgggcgcgtgaggcgcgtttacgtgctgcgcaacagcaggaggctgcatgaatattcgccgtaaaaaccgcttgtggattgcctgtgccgtgttggcagggctggcgctgactatcggtctggtgctatatgcgctgcgctcgaatatcgatctcttttatacgccgggggaaattctctacggcaagcgtgaaactcagcaaatgccggaagtcggtcagcgtctgcgcgttggcgggatggtgatgccgggtagtgtgcagcgcgatcccaattcgctgaaagtgaccttcaccatttacgatgctgaaggctcagtggatgtctcttacgaaggcattttgccggatctgttccgtgaagggcagggcgttgtggtgcagggcgaactggaaaaaggcaatcatatcctcgcgaaagaagtgctggcgaaacacgatgaaaactatacgccgccagaagttgagaaagcgatggaagctaaccaccgtcgcccggcgagtgtttataaggacccagcatcatgatgccagaaattggtaacggactgctgtgcctggcgctgggaattgcgctgctgctgtccgtgtatccgctatggggcgtggcgcgcggagatgcgcgcatgatggcgtcttcccgcttgtttgcctggctgctgtttatgtctgtggctggcgcatttctggtgctggtcaatgctttcgtggtcaacgacttcaccgtcacctatgttgccagcaactccaatacccagcttccggtgtggtatcgcgtggcggctacctggggcgcgcatgaaggctcgctactgctgtgggtgctgctgatgagcggctggacctttgcggtggcgatttttagtcagcgtattccgctggatattgtggcccgtgtactggcgataatggggatggtcagtgtcggctttttgctgttcattctctttacctctaacccgttctctcgcacgttgccgaacttcccgattgaaggtcgcgatcttaacccgctattgcaggatccggggctgatcttccatccgcctctgctttatatggggtacgtgggtttctcggtggcgtttgcttttgccattgcttctttgctgagcgggcgtctggacagcacttatgcgcgttttactcgtccgtggacgctggcagcgtggatcttcctgacgctcggcatcgtgctcggttccgcatgggcctattacgaactcggctggggtggctggtggttctgggatccggtagaaaacgcctcgtttatgccgtggctggtggggactgcgctgatgcactcactggcggtcactgaacaacgcgccagcttcaaagcgtggacattactgctggcaatcagtgccttctcgttgtgtctgttggggactttcctcgtgcgttccggcgtgctggtatcggtacacgcgtttgcgtctgatccggcgcgcggtatgtttatcctcgcctttatggtgctggtgattggcggttcgctgctgctgtttgccgcgcgtggacacaaagttcgctcacgcgtaaacaatgcgctgtggtcgcgggaatctttgctgttagcgaacaatgttttgctggtcgctgcgatgctggtggtgttgctggggacgctgctgccgttggtgcataagcaactgggactgggcagtatttcgattggcgaaccgttcttcaacaccatgtttacctggctgatggtgccgtttgcgctactgcttggtgtcggtcctctggtgcgctgggggcgggatcgcccgcgtaagatccgcaatttattgattatcgccttcatctctacgctggtgctgtcgctgctgttgccgtggctgttcgaaagcaaagttgtggcgatgacggtgctcggcctggcaatggcctgctggattgcggtgctggcaattgcggaagctgcgctacgtatttcacgcggcacgaaaaccaccttcagttattgggggatggtggcggctcaccttgggctggcagtgacaattgttggcattgcctttagccagaactatagcgttgagcgtgatgtgcgcatgaagtccggcgatagcgtcgatattcatgaatatcgcttcaccttccgtgatgtcaaagaggtgactggcccgaactggcgtggcggtgtggcgactatcggcgtaacgcgcgatggcaagccggaaacggtgctgtatgcggaaaaacgttattacaacactgccgggtcgatgatgaccgaagcggcaattgacggcggcatcacgcgtgacctgtacgcggccctcggtgaagagctggaaaacggcgcgtgggccgtgcgtctttactacaaaccatttgttcgctggatttgggcgggcgggctgatgatggcgttgggcggactgctgtgtctgtttgatcctcgctatcgtaagcgcgtgagtccgcaaaaaactgcgccggaggccgtatgaagcgcaaagtattgttaattccgttgattatcttcctggcgattgccgcggcgctgctgtggcagctggcgcgtaatgccgaaggggatgatccgaccaatctggaatcggcgctcattggcaagcctgtgccgaagtttcgtctcgaatcactggacaatccggggcagttttatcaggcggatgtgctgactcagggcaaaccagtactgcttaacgtctgggcgacctggtgtccgacctgccgtgcggaacatcaatatctgaatcagctttctgcgcagggcatccgcgtggtcggcatgaactataaagacgatcgccagaaggcaatcagctggctgaaagagctgggcaatccttacgcgctaagcctgtttgatggcgatggcatgttagggctggatctcggtgtctatggcgcgccagaaacgtttcttattgacggcaacggcatcattcgctatcgccatgcgggcgatctgaatcctcgcgtctgggaagaagagatcaagccgctgtgggagaaatacagtaaggaggccgcacaatgaggtttttattgggcgtgctgatgctgatgatctccggctcagcgctggcgaccatcgatgtgttgcagtttaaagatgaagcacaggaacaacagttccgtcagctcactgaagaactgcgctgcccgaaatgccagaacaacagcattgccgattccaactcgatgattgccaccgacctgcgtcagaaagtgtatgaactgatgcaggaaggtaaaagtaaaaaagagattgtcgattatatggtggcgcgttacggcaacttcgtcacttacgatccgccgttaacgccgctgaccgtgctgctgtgggtgctgccagtagtggctattggcattggcggttgggtcatatacgcccgctcgcggcgtcgggtacgcgtggtgccggaagcgtttcctgaacaaagcgtgccggaaggtaagcgtgccggatatgttgtttatctgccgggtattgtggtggcgttaattgtggctggcgtcagctactaccagactggcaattatcagcaggtgaaaatctggcagcaggccacggcacaggctccggcgttgctggacagggcgctggatccgaaagccgatccgctcaacgaagaagagatgtcgcgtcttgcgctggggatgcgtactcaactgcaaaaaaatccgggagatatagaaggctggattatgttgggccgcgttggcatggcgctgggtaacgccagtatcgccaccgatgcatacgccactgcgtatcgcctcgatccgaaaaacagtgatgctgcactgggatacgctgaagcgttgactcgttcatctgatcccaacgacaaccgcctcggcggtgaactgctacgtcagctggtgagaacggaccatagcaatatccgtgtgctaagcatgtatgcgtttaatgcctttgagcagcagcgatttggcgaagccgttgccgcgtgggagatgatgttgaaactcttacctgccaacgatactcgccgtgcggtgattgaacgtagtatcgcgcaggcgatgcaacatttgtcgccgcaggagagtaaataa
BBa_K1316010_sequence
1
tgtaattctcatgtgctgcataactttgcggttaatcatccggctcgtataatgtgtggacttcatatgcagccaggtttttaacagggttat
BBa_K1316011_sequence
1
tgtaattctcatgtgctgcataactttgcggttaatcatccggctcgtataatgtgtggacttcatatgcagccaggtttttaacagggttattactagaggagtgccgtaactgtcacaacttcgagtatatggatacaaccgcccagaaatcggttgccgcgaagatgcatgaccaggcggtgaaagatgggcaaacctgtattgattgccataaagggatagcgcacaagctgcccgatatgcgtgaagtcgagccaggtttttaacagggttattgcgtgggtatgcttgaagccagagagttactttgtgagcgggatgaacgaaccttatttagtggcttgtcatttacgctgaacgcaggagagtgggtacaaatcaccggtagcaacggcgcggggaagacaacgcttctccgtttgctgacggggttgtctcgccctgacgcaggcgaggttctctggcaagggcagcccttgcatcaggtacgcgacagctaccatcaaaacctgttatggataggccatcagccggggatcaaaacccggctgacggcgttagaaaatctgcacttttatcatcgcgatggcgataccgcacaatgtctggaagccctggcgcaggccgggcttgccggattcgaagatattcctgtaaatcagctctcggccgggcaacaacgccgcgtcgctttagcgcgtctgtggctgacccgtgccacgttatggatcctcgacgagccttttaccgcgattgacgttaacggtgtcgatcgtctgacccagcgtatggcgcagcatacggagcagggggggattgtgattctgactacccaccagccgctcaacgttgctgaaagtaaaattcgccgcatttcactgacgcaaacgagggccgcatgatgttctggcgcattttccgtcttgagctgcgtgtagcgtttcgccatagcgccgaaatcgccaacccgctgtggttcttcctgattgtaattaccctttttccgctcagtatcggtccggagccgcaactgctggcgcgtattgcaccgggcattatctgggttgctgcgctgctttcatccttgctggcgctggaacgactgttccgtgacgatttgcaggacggcagtcttgaacaattgatgttgttgccgttacccttgcccgccgttgtgctggcgaaggtgatggcgcactggatggtaaccggtctgccgttactcatcctttcgccactggtagcaatgctactgggaatggatgtttatggctggcaagtgatggcgctgacgctgctgctgggaacgcctacgcttggctttctcggtgcaccgggcgtggcgctgacagtgggacttaagcgcggtggtgtgctgctcagcatactggtgttaccgctgactatcccattactcatctttgccaccgccgcgatggacgcggcttctatgcatttgcccgttgacgggtatctggcaattttaggcgcgttgctggcaggcaccgcgacattaagtccttttgcgacggcggcagcgttacgaatcagcattcaataacgctggattactgatttttgttcgagtctggtatcgaaactatgtggaaaacactgcatcaactggcgatcccaccacggctgtatcaaatctgtggctggtttataccgtggctggcaattgccagtgtggtcgtgcttaccgtcggctggatctggggattcggctttgctccggctgattatcagcagggaaatagctaccgcattatctacctgcatgtgcctgcggcgatctggtcgatgggcatttatgcatcaatggcagtggcagcgtttattggccttgtctggcagatgaaaatggccaacctggcggtggcggcgatggcccccattggtgccgtgtttacctttattgccctggttaccggctctgcatggggaaaaccgatgtggggcacctggtgggtatgggatgcacgtctgacttctgaactggtgctgctgtttttgtatgtgggtgtgattgccctgtggcacgccttcgacgaccgccgtctggcgggccgtgcggcaggtatcctggtgctgattggcgtggtgaatctgccgattattcattactccgtggagtggtggaacaccctgcatcagggatcaacgcggatgcagcaaagtatcgatccggcgatgcgttcgccgctgcgctggtcgatttttggcttcctgctcctgtctgccacgctgacgctgatgcggatgcgtaatttgattttgctgatggaaaaacgccgtccgtgggtgagtgaactgatactgaaaagaggccgtaaatgacccctgcatttgcttcctggaatgaatttttcgcaatgggcggttacgccttttttgtctggctggcggtggtgatgaccgttattccgctggtggttttggtcgtgcactcggtgatgcaacatcgcgcaattctgcgtggcgtggcgcaacagcgggcgcgtgaggcgcgtttacgtgctgcgcaacagcaggaggctgcatgaatattcgccgtaaaaaccgcttgtggattgcctgtgccgtgttggcagggctggcgctgactatcggtctggtgctatatgcgctgcgctcgaatatcgatctcttttatacgccgggggaaattctctacggcaagcgtgaaactcagcaaatgccggaagtcggtcagcgtctgcgcgttggcgggatggtgatgccgggtagtgtgcagcgcgatcccaattcgctgaaagtgaccttcaccatttacgatgctgaaggctcagtggatgtctcttacgaaggcattttgccggatctgttccgtgaagggcagggcgttgtggtgcagggcgaactggaaaaaggcaatcatatcctcgcgaaagaagtgctggcgaaacacgatgaaaactatacgccgccagaagttgagaaagcgatggaagctaaccaccgtcgcccggcgagtgtttataaggacccagcatcatgatgccagaaattggtaacggactgctgtgcctggcgctgggaattgcgctgctgctgtccgtgtatccgctatggggcgtggcgcgcggagatgcgcgcatgatggcgtcttcccgcttgtttgcctggctgctgtttatgtctgtggctggcgcatttctggtgctggtcaatgctttcgtggtcaacgacttcaccgtcacctatgttgccagcaactccaatacccagcttccggtgtggtatcgcgtggcggctacctggggcgcgcatgaaggctcgctactgctgtgggtgctgctgatgagcggctggacctttgcggtggcgatttttagtcagcgtattccgctggatattgtggcccgtgtactggcgataatggggatggtcagtgtcggctttttgctgttcattctctttacctctaacccgttctctcgcacgttgccgaacttcccgattgaaggtcgcgatcttaacccgctattgcaggatccggggctgatcttccatccgcctctgctttatatggggtacgtgggtttctcggtggcgtttgcttttgccattgcttctttgctgagcgggcgtctggacagcacttatgcgcgttttactcgtccgtggacgctggcagcgtggatcttcctgacgctcggcatcgtgctcggttccgcatgggcctattacgaactcggctggggtggctggtggttctgggatccggtagaaaacgcctcgtttatgccgtggctggtggggactgcgctgatgcactcactggcggtcactgaacaacgcgccagcttcaaagcgtggacattactgctggcaatcagtgccttctcgttgtgtctgttggggactttcctcgtgcgttccggcgtgctggtatcggtacacgcgtttgcgtctgatccggcgcgcggtatgtttatcctcgcctttatggtgctggtgattggcggttcgctgctgctgtttgccgcgcgtggacacaaagttcgctcacgcgtaaacaatgcgctgtggtcgcgggaatctttgctgttagcgaacaatgttttgctggtcgctgcgatgctggtggtgttgctggggacgctgctgccgttggtgcataagcaactgggactgggcagtatttcgattggcgaaccgttcttcaacaccatgtttacctggctgatggtgccgtttgcgctactgcttggtgtcggtcctctggtgcgctgggggcgggatcgcccgcgtaagatccgcaatttattgattatcgccttcatctctacgctggtgctgtcgctgctgttgccgtggctgttcgaaagcaaagttgtggcgatgacggtgctcggcctggcaatggcctgctggattgcggtgctggcaattgcggaagctgcgctacgtatttcacgcggcacgaaaaccaccttcagttattgggggatggtggcggctcaccttgggctggcagtgacaattgttggcattgcctttagccagaactatagcgttgagcgtgatgtgcgcatgaagtccggcgatagcgtcgatattcatgaatatcgcttcaccttccgtgatgtcaaagaggtgactggcccgaactggcgtggcggtgtggcgactatcggcgtaacgcgcgatggcaagccggaaacggtgctgtatgcggaaaaacgttattacaacactgccgggtcgatgatgaccgaagcggcaattgacggcggcatcacgcgtgacctgtacgcggccctcggtgaagagctggaaaacggcgcgtgggccgtgcgtctttactacaaaccatttgttcgctggatttgggcgggcgggctgatgatggcgttgggcggactgctgtgtctgtttgatcctcgctatcgtaagcgcgtgagtccgcaaaaaactgcgccggaggccgtatgaagcgcaaagtattgttaattccgttgattatcttcctggcgattgccgcggcgctgctgtggcagctggcgcgtaatgccgaaggggatgatccgaccaatctggaatcggcgctcattggcaagcctgtgccgaagtttcgtctcgaatcactggacaatccggggcagttttatcaggcggatgtgctgactcagggcaaaccagtactgcttaacgtctgggcgacctggtgtccgacctgccgtgcggaacatcaatatctgaatcagctttctgcgcagggcatccgcgtggtcggcatgaactataaagacgatcgccagaaggcaatcagctggctgaaagagctgggcaatccttacgcgctaagcctgtttgatggcgatggcatgttagggctggatctcggtgtctatggcgcgccagaaacgtttcttattgacggcaacggcatcattcgctatcgccatgcgggcgatctgaatcctcgcgtctgggaagaagagatcaagccgctgtgggagaaatacagtaaggaggccgcacaatgaggtttttattgggcgtgctgatgctgatgatctccggctcagcgctggcgaccatcgatgtgttgcagtttaaagatgaagcacaggaacaacagttccgtcagctcactgaagaactgcgctgcccgaaatgccagaacaacagcattgccgattccaactcgatgattgccaccgacctgcgtcagaaagtgtatgaactgatgcaggaaggtaaaagtaaaaaagagattgtcgattatatggtggcgcgttacggcaacttcgtcacttacgatccgccgttaacgccgctgaccgtgctgctgtgggtgctgccagtagtggctattggcattggcggttgggtcatatacgcccgctcgcggcgtcgggtacgcgtggtgccggaagcgtttcctgaacaaagcgtgccggaaggtaagcgtgccggatatgttgtttatctgccgggtattgtggtggcgttaattgtggctggcgtcagctactaccagactggcaattatcagcaggtgaaaatctggcagcaggccacggcacaggctccggcgttgctggacagggcgctggatccgaaagccgatccgctcaacgaagaagagatgtcgcgtcttgcgctggggatgcgtactcaactgcaaaaaaatccgggagatatagaaggctggattatgttgggccgcgttggcatggcgctgggtaacgccagtatcgccaccgatgcatacgccactgcgtatcgcctcgatccgaaaaacagtgatgctgcactgggatacgctgaagcgttgactcgttcatctgatcccaacgacaaccgcctcggcggtgaactgctacgtcagctggtgagaacggaccatagcaatatccgtgtgctaagcatgtatgcgtttaatgcctttgagcagcagcgatttggcgaagccgttgccgcgtgggagatgatgttgaaactcttacctgccaacgatactcgccgtgcggtgattgaacgtagtatcgcgcaggcgatgcaacatttgtcgccgcaggagagtaaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z