LOCUS BBa_K1316012 5318 bp DNA linear 14-Nov-2024 DEFINITION T7 lacO + mtrCAB ACCESSION BBa_K1316012 VERSION BBa_K1316012.1 FEATURES Location/Qualifiers promoter 29..76 /label=T7 lacO promoter RBS 105..109 /label=RBS CDS 129..2141 /label=mtrC misc_feature 129..131 /label=start misc_feature 2139..2141 /label=stop CDS 2210..3211 /label=mtrA misc_feature 2210..2212 /label=start misc_feature 3209..3211 /label=stop CDS 3224..5317 /label=mtrB misc_feature 3224..3226 /label=start misc_feature 5315..5317 /label=stop ORIGIN 1 agatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag 61 cggataacaa ttcccctcaa gaaataattt tgtttaactt taagaaggag atatacatat 121 ggcctcttca tgaacgcaca aaaatcaaaa atcgcactgc tgctcgcagc aagtgccgtc 181 acaatggcct taaccggctg tggtggaagc gatggtaata acggcaatga tggtagtgat 241 ggtggtgagc cagcaggtag catccagacg ttaaacctag atatcactaa agtaagctat 301 gaaaatggtg cacctatggt cactgttttc gccactaacg aagccgacat gccagtgatt 361 ggtctcgcaa atttagaaat caaaaaagca ctgcaattaa taccggaagg ggcgacaggc 421 ccaggtaata gcgctaactg gcaaggctta ggctcatcaa agagctatgt cgataataaa 481 aacggtagct atacctttaa attcgacgcc ttcgatagta ataaggtctt taatgctcaa 541 ttaacgcaac gctttaacgt tgtttctgct gcgggtaaat tagcagacgg aacgaccgtt 601 cccgttgccg aaatggttga agatttcgac ggccaaggta atgcgccgca atatacaaaa 661 aatatcgtta gccacgaagt atgtgcttct tgccacgtag aaggtgaaaa gatttatcac 721 caagctactg aagtcgaaac ttgtatttct tgccacactc aagagtttgc ggatggtcgc 781 ggcaaacccc atgtcgcctt tagtcactta attcacaatg tgcataatgc caacaaagct 841 tggggcaaag acaataaaat ccctacagtt gcacaaaata ttgtccaaga taattgccaa 901 gtttgtcacg ttgaatccga catgctcacc gaggcaaaaa actggtcacg tattccaaca 961 atggaagtct gttctagctg tcacgtagac atcgattttg ctgcgggtaa aggccactct 1021 caacaactcg ataactccaa ctgtatcgcc tgccataaca gcgactggac tgctgagtta 1081 cacacagcca aaaccaccgc aactaagaac ttgattaatc aatacggtat cgagactacc 1141 tcgacaatta ataccgaaac taaagcagcc acaattagtg ttcaagttgt agatgcgaac 1201 ggtactgctg ttgatctcaa gaccatcctg cctaaagtgc aacgcttaga gatcatcacc 1261 aacgttggtc ctaataatgc aaccttaggt tatagtggca aagattcaat atttgcaatc 1321 aaaaatggag ctcttgatcc aaaagctact atcaatgatg ctggcaaact ggtttatacc 1381 actactaaag acctcaaact tggccaaaac ggcgcagaca gcgacacagc atttagcttt 1441 gtaggttggt caatgtgttc tagcgaaggt aagtttgtag actgtgcaga ccctgcattt 1501 gatggtgttg atgtaactaa gtataccggc atgaaagcgg atttagcctt tgctactttg 1561 tcaggtaaag caccaagtac tcgccacgtt gattctgtta acatgacagc ctgtgccaat 1621 tgccacactg ctgagttcga aattcacaaa ggcaaacaac atgcaggctt tgtgatgaca 1681 gagcaactat cacacaccca agatgctaac ggtaaagcga ttgtaggcct tgacgcatgt 1741 gtgacttgtc atactcctga tggcacctat agctttgcca accgtggtgc gctagagcta 1801 aaactacaca aaaaacacgt tgaagatgcc tacggcctca ttggtggcaa ttgtgcctct 1861 tgtcactcag acttcaacct tgagtctttc aagaagaaag gcgcattgaa tactgccgct 1921 gccgcagata aaacaggtct atattctacg ccgatcactg caacttgtac tacctgtcac 1981 acagttggca gccagtacat ggtccatacg aaagaaaccc tggagtcttt cggtgcagtt 2041 gttgatggca caaaagatga tgctaccagt gcggcacagt cagaaacctg tttctactgc 2101 cataccccaa cagttgcaga tcacactaaa gtgaaaatgt aatttgccca agcaggggga 2161 gctcgctccc cctttcttga attttgttgg gacaaattgg gaagcctatt atgaagaact 2221 gcctaaaaat gaaaaaccta ctgccagcac ttaccatcac aatggcaatg tcagcagtta 2281 tggcattagt cgtcacacca aacgcttatg cgtcgaagtg ggatgagaaa atgacgccag 2341 agcaagtcga agccacctta gataagaagt ttgccgaagg caactactcc cctaaaggcg 2401 ccgattcttg cttgatgtgc cataagaaat ccgaaaaagt catggacctt ttcaaaggtg 2461 tccacggtgc gattgactcc tctaagagtc caatggctgg cctgcaatgt gaggcatgcc 2521 acggcccact gggtcagcac aacaaaggcg gcaacgagcc gatgatcact tttggtaagc 2581 aatcaacctt aagtgccgac aagcaaaaca gcgtatgtat gagctgtcac caagacgata 2641 agcgtatgtc ttggaatggc ggtcaccatg acaatgccga tgttgcttgt gcttcttgtc 2701 accaagtaca cgtcgcaaaa gatcctgtgt tatctaaaaa cacggaaatg gaagtctgta 2761 ctagctgcca tacaaagcaa aaagcggata tgaataaacg ctcaagtcac ccactcaaat 2821 gggcacaaat gacctgtagc gactgtcaca atccccatgg gagcatgaca gattccgatc 2881 ttaacaagcc tagcgtgaat gatacctgtt attcctgtca cgccgaaaaa cgcggcccaa 2941 aactttggga gcatgcaccc gtcactgaga attgtgtcac ttgccacaat cctcacggta 3001 gtgtgaatga cggtatgctg aaaacccgtg cgccacagct atgtcagcaa tgtcacgcca 3061 gcgatggcca cgccagcaac gcctacttag gtaacactgg attaggttca aatgtcggtg 3121 acaatgcctt tactggtgga agaagctgct taaattgcca tagtcaggtt catggttcta 3181 accatccatc tggcaagcta ttacagcgct aaggagacga gaaaatgaaa tttaaactca 3241 atttgatcac tctagcgtta ttagccaaca caggcttggc cgtcgctgct gatggttatg 3301 gtctagcgaa tgccaatact gaaaaagtga aattatccgc atggagctgt aaaggctgcg 3361 tcgttgaaac gggcacatca ggcactgtgg gtgtcggtgt cggttataac agcgaagagg 3421 atattcgctc tgccaatgcc tttggtacat ccaatgaagt ggcgggtaaa tttgatgccg 3481 atttaaactt taaaggtgaa aagggttatc gtgccagtgt tgatgcttat caactcggta 3541 tggatggcgg tcgcttagat gtcaatgcgg gcaaacaagg ccagtacaac gtcaatgtga 3601 actatcgcca aattgctacc tacgacagca atagcgccct atcgccctac gcgggtattg 3661 gtggcaataa cctcacgtta ccggataact ggataacagc aggttcaagc aaccaaatgc 3721 cactcttgat ggacagcctc aatgccctcg aactctcact taaacgtgag cgcacggggt 3781 tgggatttga atatcaaggt gaatccctgt ggagcaccta tgttaactac atgcgtgaag 3841 agaaaaccgg cttaaaacaa gcctctggta gcttcttcaa ccaatcgatg atgttagcag 3901 agccggtgga ttacaccact gacaccattg aagcgggtgt caaactcaag ggtgatcgtt 3961 ggtttaccgc actcagttac aatgggtcaa tattcaaaaa cgaatacaac caattggact 4021 ttgaaaatgc ttttaacccc acctttggtg ctcaaaccca aggtacgatg gcactcgatc 4081 cggataacca gtcacacacc gtgtcgctga tgggacagta caacgatggc agcaacgcac 4141 tgtcgggtcg tattctgacc ggacaaatga gccaagatca ggcgttagtg acggataact 4201 accgttatgc taatcagctc aataccgatg ccgtcgatgc caaagtcgat ctactgggta 4261 tgaacctgaa agtcgttagc aaagtgagca atgatcttcg cttaacaggt agttacgatt 4321 attacgaccg tgacaataat acccaagtag aagaatggac tcagatcagc atcaacaatg 4381 tcaacggtaa ggtggcttat aacacccctt acgataatcg tacgcaacgc tttaaagttg 4441 ccgcagatta tcgcattacc cgcgatatca aactcgatgg tggttatgac ttcaaacgtg 4501 accaacgtga ttatcaagac cgtgaaacca cggatgaaaa taccgtttgg gcccgtttac 4561 gtgtaaacag cttcgatact tgggacatgt gggtaaaagg cagttacggt aaccgtgacg 4621 gctcacaata ccaagcgtct gaatggacct cttctgaaac caacagcctg ttacgtaagt 4681 acaatctggc tgaccgtgac agaactcaag tcgaagcacg gatcacccat tcgccattag 4741 aaagcctgac tatcgatgtt ggtgcccgtt acgcgttaga tgattatacc gatactgtga 4801 ttggattaac tgagtcaaaa gacaccagtt atgatgccaa catcagttat atgatcaccg 4861 ctgacttact ggcaaccgcc ttctacaatt accaaaccat tgagtctgaa caggcgggta 4921 gcagcaatta cagcacccca acgtggacag gctttataga agatcaggta gatgtggtcg 4981 gtgcaggtat cagctacaac aatctgctgg agaacaagtt acgcctagga ctggactaca 5041 cctattccaa ctccgacagt aacactcaag tcagacaagg tatcactggc gactatggtg 5101 attattttgc caaagtgcat aacattaact tatacgctca atatcaagcc accgagaaac 5161 tcgcgctgcg cttcgattac aaaattgaga actataagga caatgacgcc gcaaatgata 5221 tcgccgttga tggcatttgg aacgtcgtag gttttggtag taacagccat gactacaccg 5281 cacaaatgct gatgctgagc atgagttaca aactctaa //