BBa_K1316012 1 BBa_K1316012 T7 lacO + mtrCAB 2014-09-13T11:00:00Z 2015-05-08T01:09:50Z Caroline M. Ajo-Franklin laboratory (Goldbeck et al. ACS Synth. Biol. 2013, 2, 150−159) Combination of T7 promoter with the lac operator. For the promoter to be active both T7 RNA polymerase and the lac operon inducer (lactose or an analogue such as IPTG) must be present. This double regulatory system reduces the promoter leakage MtrCAB is an electron transport complex of the bacteria Shewanella oneidensis false false _1691_ 0 19967 9 In stock true In order to eliminate the illegal restriction sites, the entire construct was PCRed in different parts and reassembled via the Golden Gate Assembly method false Joan Cortada Garcia annotation2383248 1 stop range2383248 1 5315 5317 annotation2383242 1 mtrA range2383242 1 2210 3211 annotation2383243 1 start range2383243 1 2210 2212 annotation2383238 1 RBS range2383238 1 105 109 annotation2383237 1 T7 lacO promoter range2383237 1 29 76 annotation2383245 1 mtrB range2383245 1 3224 5317 annotation2383244 1 stop range2383244 1 3209 3211 annotation2383247 1 start range2383247 1 3224 3226 annotation2383241 1 stop range2383241 1 2139 2141 annotation2383239 1 mtrC range2383239 1 129 2141 annotation2383240 1 start range2383240 1 129 131 BBa_K1316012_sequence 1 agatcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctcaagaaataattttgtttaactttaagaaggagatatacatatggcctcttcatgaacgcacaaaaatcaaaaatcgcactgctgctcgcagcaagtgccgtcacaatggccttaaccggctgtggtggaagcgatggtaataacggcaatgatggtagtgatggtggtgagccagcaggtagcatccagacgttaaacctagatatcactaaagtaagctatgaaaatggtgcacctatggtcactgttttcgccactaacgaagccgacatgccagtgattggtctcgcaaatttagaaatcaaaaaagcactgcaattaataccggaaggggcgacaggcccaggtaatagcgctaactggcaaggcttaggctcatcaaagagctatgtcgataataaaaacggtagctatacctttaaattcgacgccttcgatagtaataaggtctttaatgctcaattaacgcaacgctttaacgttgtttctgctgcgggtaaattagcagacggaacgaccgttcccgttgccgaaatggttgaagatttcgacggccaaggtaatgcgccgcaatatacaaaaaatatcgttagccacgaagtatgtgcttcttgccacgtagaaggtgaaaagatttatcaccaagctactgaagtcgaaacttgtatttcttgccacactcaagagtttgcggatggtcgcggcaaaccccatgtcgcctttagtcacttaattcacaatgtgcataatgccaacaaagcttggggcaaagacaataaaatccctacagttgcacaaaatattgtccaagataattgccaagtttgtcacgttgaatccgacatgctcaccgaggcaaaaaactggtcacgtattccaacaatggaagtctgttctagctgtcacgtagacatcgattttgctgcgggtaaaggccactctcaacaactcgataactccaactgtatcgcctgccataacagcgactggactgctgagttacacacagccaaaaccaccgcaactaagaacttgattaatcaatacggtatcgagactacctcgacaattaataccgaaactaaagcagccacaattagtgttcaagttgtagatgcgaacggtactgctgttgatctcaagaccatcctgcctaaagtgcaacgcttagagatcatcaccaacgttggtcctaataatgcaaccttaggttatagtggcaaagattcaatatttgcaatcaaaaatggagctcttgatccaaaagctactatcaatgatgctggcaaactggtttataccactactaaagacctcaaacttggccaaaacggcgcagacagcgacacagcatttagctttgtaggttggtcaatgtgttctagcgaaggtaagtttgtagactgtgcagaccctgcatttgatggtgttgatgtaactaagtataccggcatgaaagcggatttagcctttgctactttgtcaggtaaagcaccaagtactcgccacgttgattctgttaacatgacagcctgtgccaattgccacactgctgagttcgaaattcacaaaggcaaacaacatgcaggctttgtgatgacagagcaactatcacacacccaagatgctaacggtaaagcgattgtaggccttgacgcatgtgtgacttgtcatactcctgatggcacctatagctttgccaaccgtggtgcgctagagctaaaactacacaaaaaacacgttgaagatgcctacggcctcattggtggcaattgtgcctcttgtcactcagacttcaaccttgagtctttcaagaagaaaggcgcattgaatactgccgctgccgcagataaaacaggtctatattctacgccgatcactgcaacttgtactacctgtcacacagttggcagccagtacatggtccatacgaaagaaaccctggagtctttcggtgcagttgttgatggcacaaaagatgatgctaccagtgcggcacagtcagaaacctgtttctactgccataccccaacagttgcagatcacactaaagtgaaaatgtaatttgcccaagcagggggagctcgctccccctttcttgaattttgttgggacaaattgggaagcctattatgaagaactgcctaaaaatgaaaaacctactgccagcacttaccatcacaatggcaatgtcagcagttatggcattagtcgtcacaccaaacgcttatgcgtcgaagtgggatgagaaaatgacgccagagcaagtcgaagccaccttagataagaagtttgccgaaggcaactactcccctaaaggcgccgattcttgcttgatgtgccataagaaatccgaaaaagtcatggaccttttcaaaggtgtccacggtgcgattgactcctctaagagtccaatggctggcctgcaatgtgaggcatgccacggcccactgggtcagcacaacaaaggcggcaacgagccgatgatcacttttggtaagcaatcaaccttaagtgccgacaagcaaaacagcgtatgtatgagctgtcaccaagacgataagcgtatgtcttggaatggcggtcaccatgacaatgccgatgttgcttgtgcttcttgtcaccaagtacacgtcgcaaaagatcctgtgttatctaaaaacacggaaatggaagtctgtactagctgccatacaaagcaaaaagcggatatgaataaacgctcaagtcacccactcaaatgggcacaaatgacctgtagcgactgtcacaatccccatgggagcatgacagattccgatcttaacaagcctagcgtgaatgatacctgttattcctgtcacgccgaaaaacgcggcccaaaactttgggagcatgcacccgtcactgagaattgtgtcacttgccacaatcctcacggtagtgtgaatgacggtatgctgaaaacccgtgcgccacagctatgtcagcaatgtcacgccagcgatggccacgccagcaacgcctacttaggtaacactggattaggttcaaatgtcggtgacaatgcctttactggtggaagaagctgcttaaattgccatagtcaggttcatggttctaaccatccatctggcaagctattacagcgctaaggagacgagaaaatgaaatttaaactcaatttgatcactctagcgttattagccaacacaggcttggccgtcgctgctgatggttatggtctagcgaatgccaatactgaaaaagtgaaattatccgcatggagctgtaaaggctgcgtcgttgaaacgggcacatcaggcactgtgggtgtcggtgtcggttataacagcgaagaggatattcgctctgccaatgcctttggtacatccaatgaagtggcgggtaaatttgatgccgatttaaactttaaaggtgaaaagggttatcgtgccagtgttgatgcttatcaactcggtatggatggcggtcgcttagatgtcaatgcgggcaaacaaggccagtacaacgtcaatgtgaactatcgccaaattgctacctacgacagcaatagcgccctatcgccctacgcgggtattggtggcaataacctcacgttaccggataactggataacagcaggttcaagcaaccaaatgccactcttgatggacagcctcaatgccctcgaactctcacttaaacgtgagcgcacggggttgggatttgaatatcaaggtgaatccctgtggagcacctatgttaactacatgcgtgaagagaaaaccggcttaaaacaagcctctggtagcttcttcaaccaatcgatgatgttagcagagccggtggattacaccactgacaccattgaagcgggtgtcaaactcaagggtgatcgttggtttaccgcactcagttacaatgggtcaatattcaaaaacgaatacaaccaattggactttgaaaatgcttttaaccccacctttggtgctcaaacccaaggtacgatggcactcgatccggataaccagtcacacaccgtgtcgctgatgggacagtacaacgatggcagcaacgcactgtcgggtcgtattctgaccggacaaatgagccaagatcaggcgttagtgacggataactaccgttatgctaatcagctcaataccgatgccgtcgatgccaaagtcgatctactgggtatgaacctgaaagtcgttagcaaagtgagcaatgatcttcgcttaacaggtagttacgattattacgaccgtgacaataatacccaagtagaagaatggactcagatcagcatcaacaatgtcaacggtaaggtggcttataacaccccttacgataatcgtacgcaacgctttaaagttgccgcagattatcgcattacccgcgatatcaaactcgatggtggttatgacttcaaacgtgaccaacgtgattatcaagaccgtgaaaccacggatgaaaataccgtttgggcccgtttacgtgtaaacagcttcgatacttgggacatgtgggtaaaaggcagttacggtaaccgtgacggctcacaataccaagcgtctgaatggacctcttctgaaaccaacagcctgttacgtaagtacaatctggctgaccgtgacagaactcaagtcgaagcacggatcacccattcgccattagaaagcctgactatcgatgttggtgcccgttacgcgttagatgattataccgatactgtgattggattaactgagtcaaaagacaccagttatgatgccaacatcagttatatgatcaccgctgacttactggcaaccgccttctacaattaccaaaccattgagtctgaacaggcgggtagcagcaattacagcaccccaacgtggacaggctttatagaagatcaggtagatgtggtcggtgcaggtatcagctacaacaatctgctggagaacaagttacgcctaggactggactacacctattccaactccgacagtaacactcaagtcagacaaggtatcactggcgactatggtgattattttgccaaagtgcataacattaacttatacgctcaatatcaagccaccgagaaactcgcgctgcgcttcgattacaaaattgagaactataaggacaatgacgccgcaaatgatatcgccgttgatggcatttggaacgtcgtaggttttggtagtaacagccatgactacaccgcacaaatgctgatgctgagcatgagttacaaactctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z