BBa_K1316015 1 BBa_K1316015 pRhamnose regulating csgB and csgA curli proteins 2014-09-28T11:00:00Z 2015-05-08T01:09:50Z csgA and csgB were PCRed out of the K540000 Lyon 2011 iGEM part. This part is meant to express the csgB and csgA curli genes, which respectively code for the production of the curli protein that is anchored to the cell membrane (CsgB); and for the major curli protein (CsgA), which forms long fibrils with other CsgA monomers in a self-assembly process. These CsgA long fibrils bind to the anchored CsgB protein forming the amyloid-like curli structure. The expression of these two genes will, then, occur at the same time and under the same conditions (in the presence of Rhamnose in this case) This construct was designed as a negative control for BBa_K1316013 and BBa_K1316014, to see if expressing CsgA constitutively has a positive impact on the speed of curli formation false false _1691_ 0 19967 9 In stock false This construct was designed as a negative control for BBa_K1316013 and BBa_K1316014, to see if expressing CsgA constitutively has a positive impact on the speed of curli formation false Joan Cortada Garcia annotation2399913 1 Rhamnose promoter range2399913 1 1 122 annotation2399917 1 start range2399917 1 161 163 annotation2399921 1 start range2399921 1 657 659 annotation2399916 1 csgB range2399916 1 161 616 annotation2399918 1 stop range2399918 1 614 616 annotation2399914 1 rbs range2399914 1 148 159 annotation2399922 1 stop range2399922 1 1110 1112 annotation2399919 1 csgA range2399919 1 657 1112 BBa_K1316015_sequence 1 agccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagagcagcaaggaaatactagaaggaggtatataatgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaaaaaacggcaattgtagtgcagagacagtcgcaaatggctattcgcgtgacacaacgttaatttccattcgacttttaaatcaatccgatgggggttttacatgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgcagcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactaactactagaaggagg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z