LOCUS BBa_K1316017 5333 bp DNA linear 22-Dec-2024 DEFINITION T7 lacO + mtrCAB with His tag ACCESSION BBa_K1316017 VERSION BBa_K1316017.1 FEATURES Location/Qualifiers promoter 27..82 /label=T7 lacO promoter RBS 103..108 /label=RBS CDS 128..2140 /label=mtrC misc_feature 2138..2140 /label=stop CDS 2208..3210 /label=mtrA misc_feature 3208..3210 /label=stop CDS 3223..5333 /label=mtrB misc_feature 5313..5331 /label=HisTag ORIGIN 1 atcgagatcg atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg 61 gataacaatt cccctcaaga aataattttg tttaacttta agaaggagat atacatatgg 121 cctcttcatg aacgcacaaa aatcaaaaat cgcactgctg ctcgcagcaa gtgccgtcac 181 aatggcctta accggctgtg gtggaagcga tggtaataac ggcaatgatg gtagtgatgg 241 tggtgagcca gcaggtagca tccagacgtt aaacctagat atcactaaag taagctatga 301 aaatggtgca cctatggtca ctgttttcgc cactaacgaa gccgacatgc cagtgattgg 361 tctcgcaaat ttagaaatca aaaaagcact gcaattaata ccggaagggg cgacaggccc 421 aggtaatagc gctaactggc aaggcttagg ctcatcaaag agctatgtcg ataataaaaa 481 cggtagctat acctttaaat tcgacgcctt cgatagtaat aaggtcttta atgctcaatt 541 aacgcaacgc tttaacgttg tttctgctgc gggtaaatta gcagacggaa cgaccgttcc 601 cgttgccgaa atggttgaag atttcgacgg ccaaggtaat gcgccgcaat atacaaaaaa 661 tatcgttagc cacgaagtat gtgcttcttg ccacgtagaa ggtgaaaaga tttatcacca 721 agctactgaa gtcgaaactt gtatttcttg ccacactcaa gagtttgcgg atggtcgcgg 781 caaaccccat gtcgccttta gtcacttaat tcacaatgtg cataatgcca acaaagcttg 841 gggcaaagac aataaaatcc ctacagttgc acaaaatatt gtccaagata attgccaagt 901 ttgtcacgtt gaatccgaca tgctcaccga ggcaaaaaac tggtcacgta ttccaacaat 961 ggaagtctgt tctagctgtc acgtagacat cgattttgct gcgggtaaag gccactctca 1021 acaactcgat aactccaact gtatcgcctg ccataacagc gactggactg ctgagttaca 1081 cacagccaaa accaccgcaa ctaagaactt gattaatcaa tacggtatcg agactacctc 1141 gacaattaat accgaaacta aagcagccac aattagtgtt caagttgtag atgcgaacgg 1201 tactgctgtt gatctcaaga ccatcctgcc taaagtgcaa cgcttagaga tcatcaccaa 1261 cgttggtcct aataatgcaa ccttaggtta tagtggcaaa gattcaatat ttgcaatcaa 1321 aaatggagct cttgatccaa aagctactat caatgatgct ggcaaactgg tttataccac 1381 tactaaagac ctcaaacttg gccaaaacgg cgcagacagc gacacagcat ttagctttgt 1441 aggttggtca atgtgttcta gcgaaggtaa gtttgtagac tgtgcagacc ctgcatttga 1501 tggtgttgat gtaactaagt ataccggcat gaaagcggat ttagcctttg ctactttgtc 1561 aggtaaagca ccaagtactc gccacgttga ttctgttaac atgacagcct gtgccaattg 1621 ccacactgct gagttcgaaa ttcacaaagg caaacaacat gcaggctttg tgatgacaga 1681 gcaactatca cacacccaag atgctaacgg taaagcgatt gtaggccttg acgcatgtgt 1741 gacttgtcat actcctgatg gcacctatag ctttgccaac cgtggtgcgc tagagctaaa 1801 actacacaaa aaacacgttg aagatgccta cggcctcatt ggtggcaatt gtgcctcttg 1861 tcactcagac ttcaaccttg agtctttcaa gaagaaaggc gcattgaata ctgccgctgc 1921 cgcagataaa acaggtctat attctacgcc gatcactgca acttgtacta cctgtcacac 1981 agttggcagc cagtacatgg tccatacgaa agaaaccctg gagtctttcg gtgcagttgt 2041 tgatggcaca aaagatgatg ctaccagtgc ggcacagtca gaaacctgtt tctactgcca 2101 taccccaaca gttgcagatc acactaaagt gaaaatgtaa tttgcccaag cagggggagc 2161 tcgctccccc tttcttgaat tttgttggga caaattggga agcctattat gaagaactgc 2221 ctaaaaatga aaaacctact gccagcactt accatcacaa tggcaatgtc agcagttatg 2281 gcattagtcg tcacaccaaa cgcttatgcg tcgaagtggg atgagaaaat gacgccagag 2341 caagtcgaag ccaccttaga taagaagttt gccgaaggca actactcccc taaaggcgcc 2401 gattcttgct tgatgtgcca taagaaatcc gaaaaagtca tggacctttt caaaggtgtc 2461 cacggtgcga ttgactcctc taagagtcca atggctggcc tgcaatgtga ggcatgccac 2521 ggcccactgg gtcagcacaa caaaggcggc aacgagccga tgatcacttt tggtaagcaa 2581 tcaaccttaa gtgccgacaa gcaaaacagc gtatgtatga gctgtcacca agacgataag 2641 cgtatgtctt ggaatggcgg tcaccatgac aatgccgatg ttgcttgtgc ttcttgtcac 2701 caagtacacg tcgcaaaaga tcctgtgtta tctaaaaaca cggaaatgga agtctgtact 2761 agctgccata caaagcaaaa agcggatatg aataaacgct caagtcaccc actcaaatgg 2821 gcacaaatga cctgtagcga ctgtcacaat ccccatggga gcatgacaga ttccgatctt 2881 aacaagccta gcgtgaatga tacctgttat tcctgtcacg ccgaaaaacg cggcccaaaa 2941 ctttgggagc atgcacccgt cactgagaat tgtgtcactt gccacaatcc tcacggtagt 3001 gtgaatgacg gtatgctgaa aacccgtgcg ccacagctat gtcagcaatg tcacgccagc 3061 gatggccacg ccagcaacgc ctacttaggt aacactggat taggttcaaa tgtcggtgac 3121 aatgccttta ctggtggaag aagctgctta aattgccata gtcaggttca tggttctaac 3181 catccatctg gcaagctatt acagcgctaa ggagacgaga aaatgaaatt taaactcaat 3241 ttgatcactc tagcgttatt agccaacaca ggcttggccg tcgctgctga tggttatggt 3301 ctagcgaatg ccaatactga aaaagtgaaa ttatccgcat ggagctgtaa aggctgcgtc 3361 gttgaaacgg gcacatcagg cactgtgggt gtcggtgtcg gttataacag cgaagaggat 3421 attcgctctg ccaatgcctt tggtacatcc aatgaagtgg cgggtaaatt tgatgccgat 3481 ttaaacttta aaggtgaaaa gggttatcgt gccagtgttg atgcttatca actcggtatg 3541 gatggcggtc gcttagatgt caatgcgggc aaacaaggcc agtacaacgt caatgtgaac 3601 tatcgccaaa ttgctaccta cgacagcaat agcgccctat cgccctacgc gggtattggt 3661 ggcaataacc tcacgttacc ggataactgg ataacagcag gttcaagcaa ccaaatgcca 3721 ctcttgatgg acagcctcaa tgccctcgaa ctctcactta aacgtgagcg cacggggttg 3781 ggatttgaat atcaaggtga atccctgtgg agcacctatg ttaactacat gcgtgaagag 3841 aaaaccggct taaaacaagc ctctggtagc ttcttcaacc aatcgatgat gttagcagag 3901 ccggtggatt acaccactga caccattgaa gcgggtgtca aactcaaggg tgatcgttgg 3961 tttaccgcac tcagttacaa tgggtcaata ttcaaaaacg aatacaacca attggacttt 4021 gaaaatgctt ttaaccccac ctttggtgct caaacccaag gtacgatggc actcgatccg 4081 gataaccagt cacacaccgt gtcgctgatg ggacagtaca acgatggcag caacgcactg 4141 tcgggtcgta ttctgaccgg acaaatgagc caagatcagg cgttagtgac ggataactac 4201 cgttatgcta atcagctcaa taccgatgcc gtcgatgcca aagtcgatct actgggtatg 4261 aacctgaaag tcgttagcaa agtgagcaat gatcttcgct taacaggtag ttacgattat 4321 tacgaccgtg acaataatac ccaagtagaa gaatggactc agatcagcat caacaatgtc 4381 aacggtaagg tggcttataa caccccttac gataatcgta cgcaacgctt taaagttgcc 4441 gcagattatc gcattacccg cgatatcaaa ctcgatggtg gttatgactt caaacgtgac 4501 caacgtgatt atcaagaccg tgaaaccacg gatgaaaata ccgtttgggc ccgtttacgt 4561 gtaaacagct tcgatacttg ggacatgtgg gtaaaaggca gttacggtaa ccgtgacggc 4621 tcacaatacc aagcgtctga atggacctct tctgaaacca acagcctgtt acgtaagtac 4681 aatctggctg accgtgacag aactcaagtc gaagcacgga tcacccattc gccattagaa 4741 agcctgacta tcgatgttgg tgcccgttac gcgttagatg attataccga tactgtgatt 4801 ggattaactg agtcaaaaga caccagttat gatgccaaca tcagttatat gatcaccgct 4861 gacttactgg caaccgcctt ctacaattac caaaccattg agtctgaaca ggcgggtagc 4921 agcaattaca gcaccccaac gtggacaggc tttatagaag atcaggtaga tgtggtcggt 4981 gcaggtatca gctacaacaa tctgctggag aacaagttac gcctaggact ggactacacc 5041 tattccaact ccgacagtaa cactcaagtc agacaaggta tcactggcga ctatggtgat 5101 tattttgcca aagtgcataa cattaactta tacgctcaat atcaagccac cgagaaactc 5161 gcgctgcgct tcgattacaa aattgagaac tataaggaca atgacgccgc aaatgatatc 5221 gccgttgatg gcatttggaa cgtcgtaggt tttggtagta acagccatga ctacaccgca 5281 caaatgctga tgctgagcat gagttacaaa ctccatcatc accatcacca caa //