BBa_K1316017 1 BBa_K1316017 T7 lacO + mtrCAB with His tag 2014-09-28T11:00:00Z 2015-05-08T01:09:50Z Caroline M. Ajo-Franklin laboratory (Goldbeck et al. ACS Synth. Biol. 2013, 2, 150−159) Combination of T7 promoter with the lac operator. For the promoter to be active both T7 RNA polymerase and the lac operon inducer (lactose or an analogue such as IPTG) must be present. This double regulatory system reduces the promoter leakage MtrCAB is an electron transport complex of the bacteria Shewanella oneidensis A His tag was placed at the C-terminus of the MtrB protein in order to easily be able to detect and purify this protein. false false _1691_ 0 19967 9 It's complicated false A His tag was placed at the C-terminus of the MtrB protein in order to easily be able to detect and purify this protein. The big gene complex is supposed to be very toxic for the cell, thus, having a low leakage promoter such as the T7 lacO is crucial to ensure the viability of the cells. false Joan Cortada Garcia annotation2389663 1 stop range2389663 1 3208 3210 annotation2389661 1 stop range2389661 1 2138 2140 annotation2389660 1 mtrC range2389660 1 128 2140 annotation2389665 1 HisTag range2389665 1 5313 5331 annotation2389658 1 T7 lacO promoter range2389658 1 27 82 annotation2389659 1 RBS range2389659 1 103 108 annotation2389664 1 mtrB range2389664 1 3223 5333 annotation2389662 1 mtrA range2389662 1 2208 3210 BBa_K1316017_sequence 1 atcgagatcgatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctcaagaaataattttgtttaactttaagaaggagatatacatatggcctcttcatgaacgcacaaaaatcaaaaatcgcactgctgctcgcagcaagtgccgtcacaatggccttaaccggctgtggtggaagcgatggtaataacggcaatgatggtagtgatggtggtgagccagcaggtagcatccagacgttaaacctagatatcactaaagtaagctatgaaaatggtgcacctatggtcactgttttcgccactaacgaagccgacatgccagtgattggtctcgcaaatttagaaatcaaaaaagcactgcaattaataccggaaggggcgacaggcccaggtaatagcgctaactggcaaggcttaggctcatcaaagagctatgtcgataataaaaacggtagctatacctttaaattcgacgccttcgatagtaataaggtctttaatgctcaattaacgcaacgctttaacgttgtttctgctgcgggtaaattagcagacggaacgaccgttcccgttgccgaaatggttgaagatttcgacggccaaggtaatgcgccgcaatatacaaaaaatatcgttagccacgaagtatgtgcttcttgccacgtagaaggtgaaaagatttatcaccaagctactgaagtcgaaacttgtatttcttgccacactcaagagtttgcggatggtcgcggcaaaccccatgtcgcctttagtcacttaattcacaatgtgcataatgccaacaaagcttggggcaaagacaataaaatccctacagttgcacaaaatattgtccaagataattgccaagtttgtcacgttgaatccgacatgctcaccgaggcaaaaaactggtcacgtattccaacaatggaagtctgttctagctgtcacgtagacatcgattttgctgcgggtaaaggccactctcaacaactcgataactccaactgtatcgcctgccataacagcgactggactgctgagttacacacagccaaaaccaccgcaactaagaacttgattaatcaatacggtatcgagactacctcgacaattaataccgaaactaaagcagccacaattagtgttcaagttgtagatgcgaacggtactgctgttgatctcaagaccatcctgcctaaagtgcaacgcttagagatcatcaccaacgttggtcctaataatgcaaccttaggttatagtggcaaagattcaatatttgcaatcaaaaatggagctcttgatccaaaagctactatcaatgatgctggcaaactggtttataccactactaaagacctcaaacttggccaaaacggcgcagacagcgacacagcatttagctttgtaggttggtcaatgtgttctagcgaaggtaagtttgtagactgtgcagaccctgcatttgatggtgttgatgtaactaagtataccggcatgaaagcggatttagcctttgctactttgtcaggtaaagcaccaagtactcgccacgttgattctgttaacatgacagcctgtgccaattgccacactgctgagttcgaaattcacaaaggcaaacaacatgcaggctttgtgatgacagagcaactatcacacacccaagatgctaacggtaaagcgattgtaggccttgacgcatgtgtgacttgtcatactcctgatggcacctatagctttgccaaccgtggtgcgctagagctaaaactacacaaaaaacacgttgaagatgcctacggcctcattggtggcaattgtgcctcttgtcactcagacttcaaccttgagtctttcaagaagaaaggcgcattgaatactgccgctgccgcagataaaacaggtctatattctacgccgatcactgcaacttgtactacctgtcacacagttggcagccagtacatggtccatacgaaagaaaccctggagtctttcggtgcagttgttgatggcacaaaagatgatgctaccagtgcggcacagtcagaaacctgtttctactgccataccccaacagttgcagatcacactaaagtgaaaatgtaatttgcccaagcagggggagctcgctccccctttcttgaattttgttgggacaaattgggaagcctattatgaagaactgcctaaaaatgaaaaacctactgccagcacttaccatcacaatggcaatgtcagcagttatggcattagtcgtcacaccaaacgcttatgcgtcgaagtgggatgagaaaatgacgccagagcaagtcgaagccaccttagataagaagtttgccgaaggcaactactcccctaaaggcgccgattcttgcttgatgtgccataagaaatccgaaaaagtcatggaccttttcaaaggtgtccacggtgcgattgactcctctaagagtccaatggctggcctgcaatgtgaggcatgccacggcccactgggtcagcacaacaaaggcggcaacgagccgatgatcacttttggtaagcaatcaaccttaagtgccgacaagcaaaacagcgtatgtatgagctgtcaccaagacgataagcgtatgtcttggaatggcggtcaccatgacaatgccgatgttgcttgtgcttcttgtcaccaagtacacgtcgcaaaagatcctgtgttatctaaaaacacggaaatggaagtctgtactagctgccatacaaagcaaaaagcggatatgaataaacgctcaagtcacccactcaaatgggcacaaatgacctgtagcgactgtcacaatccccatgggagcatgacagattccgatcttaacaagcctagcgtgaatgatacctgttattcctgtcacgccgaaaaacgcggcccaaaactttgggagcatgcacccgtcactgagaattgtgtcacttgccacaatcctcacggtagtgtgaatgacggtatgctgaaaacccgtgcgccacagctatgtcagcaatgtcacgccagcgatggccacgccagcaacgcctacttaggtaacactggattaggttcaaatgtcggtgacaatgcctttactggtggaagaagctgcttaaattgccatagtcaggttcatggttctaaccatccatctggcaagctattacagcgctaaggagacgagaaaatgaaatttaaactcaatttgatcactctagcgttattagccaacacaggcttggccgtcgctgctgatggttatggtctagcgaatgccaatactgaaaaagtgaaattatccgcatggagctgtaaaggctgcgtcgttgaaacgggcacatcaggcactgtgggtgtcggtgtcggttataacagcgaagaggatattcgctctgccaatgcctttggtacatccaatgaagtggcgggtaaatttgatgccgatttaaactttaaaggtgaaaagggttatcgtgccagtgttgatgcttatcaactcggtatggatggcggtcgcttagatgtcaatgcgggcaaacaaggccagtacaacgtcaatgtgaactatcgccaaattgctacctacgacagcaatagcgccctatcgccctacgcgggtattggtggcaataacctcacgttaccggataactggataacagcaggttcaagcaaccaaatgccactcttgatggacagcctcaatgccctcgaactctcacttaaacgtgagcgcacggggttgggatttgaatatcaaggtgaatccctgtggagcacctatgttaactacatgcgtgaagagaaaaccggcttaaaacaagcctctggtagcttcttcaaccaatcgatgatgttagcagagccggtggattacaccactgacaccattgaagcgggtgtcaaactcaagggtgatcgttggtttaccgcactcagttacaatgggtcaatattcaaaaacgaatacaaccaattggactttgaaaatgcttttaaccccacctttggtgctcaaacccaaggtacgatggcactcgatccggataaccagtcacacaccgtgtcgctgatgggacagtacaacgatggcagcaacgcactgtcgggtcgtattctgaccggacaaatgagccaagatcaggcgttagtgacggataactaccgttatgctaatcagctcaataccgatgccgtcgatgccaaagtcgatctactgggtatgaacctgaaagtcgttagcaaagtgagcaatgatcttcgcttaacaggtagttacgattattacgaccgtgacaataatacccaagtagaagaatggactcagatcagcatcaacaatgtcaacggtaaggtggcttataacaccccttacgataatcgtacgcaacgctttaaagttgccgcagattatcgcattacccgcgatatcaaactcgatggtggttatgacttcaaacgtgaccaacgtgattatcaagaccgtgaaaccacggatgaaaataccgtttgggcccgtttacgtgtaaacagcttcgatacttgggacatgtgggtaaaaggcagttacggtaaccgtgacggctcacaataccaagcgtctgaatggacctcttctgaaaccaacagcctgttacgtaagtacaatctggctgaccgtgacagaactcaagtcgaagcacggatcacccattcgccattagaaagcctgactatcgatgttggtgcccgttacgcgttagatgattataccgatactgtgattggattaactgagtcaaaagacaccagttatgatgccaacatcagttatatgatcaccgctgacttactggcaaccgccttctacaattaccaaaccattgagtctgaacaggcgggtagcagcaattacagcaccccaacgtggacaggctttatagaagatcaggtagatgtggtcggtgcaggtatcagctacaacaa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igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z