BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_K1317004 1 BBa_K1317004 Fusion protein RFP + Elastin like polypeptide (ELP) 2014-10-06T11:00:00Z 2015-05-08T01:09:50Z The source of this part is BBa_K1317003. The ELP presents specific properties: it precipitates at high temperature. This property can be used for thermal cycling purification for instance. By successive cycles of temperature, ELP can be purified from a mix of proteins. This special property has been used to create a composite part for the iGEM registry. It is a proof of concept of the use of this part as a tool for protein purification by fusing ELP to another protein. The protein used as an example is RFP, to ease vizualisation of the process. false false _1692_ 0 20045 9 Not in stock false This part has been assembled by chew back assembly (cf wiki page: ) In-silico cloning and design have been performed on Genome Compiler. false Mounir Benkoulouche component2423566 1 BBa_E1010 component2423563 1 BBa_K1317003 annotation2423563 1 BBa_K1317003 range2423563 1 1 333 annotation2423566 1 BBa_E1010 range2423566 1 340 1045 BBa_K1317003 1 BBa_K1317003 CDS for Elastin like polypeptide (ELP) 2014-10-06T11:00:00Z 2015-05-08T01:09:50Z Next Next false false _1692_ 0 20045 9 It's complicated false Next false Mounir Benkoulouche annotation2399396 1 ELP20 range2399396 1 6 332 annotation2399398 1 Stop codon range2399398 1 330 332 annotation2399397 1 Start Codon range2399397 1 6 8 annotation2399399 1 Aromatic (dosage) range2399399 1 9 11 annotation2399400 1 NheI range2399400 1 312 317 annotation2399855 1 Chew Back range2399855 1 324 329 BBa_K1317003_sequence 1 ctagaatgtgggggcccggaggagtaggtcccttaggggtggggcccttgcaacagggccctggaggttacggccccggtggcgtgggcccacttggtgtgggacctctccagcaaggccctggaggttatggtccggggggagtcggcccactaggtgttgggccgttgcagcaagggccggggggttatggaccaggtggcgttggtccgttaggagtaggaccgttgcagcagggcccgggtggctatggacctggcggcgttggtccacttggtgtcggtcctctccagcaagggccggggggttacgctagcttatcatctagctaaa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K1317004_sequence 1 ctagaatgtgggggcccggaggagtaggtcccttaggggtggggcccttgcaacagggccctggaggttacggccccggtggcgtgggcccacttggtgtgggacctctccagcaaggccctggaggttatggtccggggggagtcggcccactaggtgttgggccgttgcagcaagggccggggggttatggaccaggtggcgttggtccgttaggagtaggaccgttgcagcagggcccgggtggctatggacctggcggcgttggtccacttggtgtcggtcctctccagcaagggccggggggttacgctagcttatcatctagctaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z