LOCUS       BBa_K1319004             726 bp    DNA     linear       22-Dec-2024
DEFINITION  TEV protease with anti-self cleavage mutation S219V, codon 
            optimized for E. coli
ACCESSION   BBa_K1319004
VERSION     BBa_K1319004.1  
FEATURES             Location/Qualifiers
     CDS             1..726
                     /label=TEV protease
     misc_feature    655..657
                     /label=S219V
ORIGIN
        1 ggcgaaagcc tgttcaaagg tccgcgtgac tacaacccga ttagctcgac gatctgccac
       61 ctgacgaacg aaagcgacgg ccacaccacg agcctgtatg gcatcggttt tggcccgttc
      121 attatcacga acaaacacct gtttcgtcgc aacaatggta ccctgctggt gcagtctctg
      181 catggcgtgt ttaaagttaa aaataccacg accctgcaac aacacctgat cgatggtcgt
      241 gacatgatta tcattcgcat gccgaaagat tttccgccgt tcccgcagaa actgaaattc
      301 cgtgaaccgc aacgtgaaga acgcatttgc ctggtcacga ccaactttca gaccaaatca
      361 atgagctcta tggttagcga tacgtcttgt acctttccga gttccgacgg catcttctgg
      421 aaacattgga ttcagaccaa agatggtcaa tgcggcagtc cgctggtttc cacccgtgac
      481 ggtttcatcg tcggcattca ctcagcgtcg aactttacga ataccaacaa ttacttcacg
      541 tccgttccga aaaactttat ggaactgctg accaatcagg aagcgcagca atgggtgtca
      601 ggttggcgcc tgaatgccga ttcggttctg tggggcggtc acaaagtctt tatggtgaaa
      661 ccggaagaac cgttccagcc ggtcaaagaa gcaacccaac tgatgaatga actggtctac
      721 tcccaa
//