BBa_K1319004 1 BBa_K1319004 TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli 2014-09-24T11:00:00Z 2015-05-08T01:09:50Z The part was codon optimized using the genomic amino acid sequence, designed for RFC25 and mutated S219V for anti-self cleavage properties. This part is a TEV protease in RFC25 that was optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation. false false _1694_ 0 19522 9 In stock false The part does not contain start or stop codons, because it was designed for RFC25 compability false Florian Gohr, Michael Osthege annotation2386539 1 TEV protease range2386539 1 1 726 annotation2386540 1 S219V range2386540 1 655 657 BBa_K1319004_sequence 1 ggcgaaagcctgttcaaaggtccgcgtgactacaacccgattagctcgacgatctgccacctgacgaacgaaagcgacggccacaccacgagcctgtatggcatcggttttggcccgttcattatcacgaacaaacacctgtttcgtcgcaacaatggtaccctgctggtgcagtctctgcatggcgtgtttaaagttaaaaataccacgaccctgcaacaacacctgatcgatggtcgtgacatgattatcattcgcatgccgaaagattttccgccgttcccgcagaaactgaaattccgtgaaccgcaacgtgaagaacgcatttgcctggtcacgaccaactttcagaccaaatcaatgagctctatggttagcgatacgtcttgtacctttccgagttccgacggcatcttctggaaacattggattcagaccaaagatggtcaatgcggcagtccgctggtttccacccgtgacggtttcatcgtcggcattcactcagcgtcgaactttacgaataccaacaattacttcacgtccgttccgaaaaactttatggaactgctgaccaatcaggaagcgcagcaatgggtgtcaggttggcgcctgaatgccgattcggttctgtggggcggtcacaaagtctttatggtgaaaccggaagaaccgttccagccggtcaaagaagcaacccaactgatgaatgaactggtctactcccaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z