LOCUS       BBa_K1319008             930 bp    DNA     linear       22-Nov-2024
DEFINITION  IPTG-induced and T7-driven expression of TEV protease
ACCESSION   BBa_K1319008
VERSION     BBa_K1319008.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    24..31
                     /label=BBa_G0002
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    50..52
                     /label=start
     misc_feature    44..58
                     /label=BBa_K1319106
     CDS             59..784
                     /label=TEV protease
     misc_feature    713..715
                     /label=S219V
     CDS             59..784
                     /label=BBa_K1319004
     misc_feature    791..793
                     /label=stop
     misc_feature    785..801
                     /label=BBa_K1319107
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     terminator      802..930
                     /label=BBa_B0015
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggccggcgg
       61 cgaaagcctg ttcaaaggtc cgcgtgacta caacccgatt agctcgacga tctgccacct
      121 gacgaacgaa agcgacggcc acaccacgag cctgtatggc atcggttttg gcccgttcat
      181 tatcacgaac aaacacctgt ttcgtcgcaa caatggtacc ctgctggtgc agtctctgca
      241 tggcgtgttt aaagttaaaa ataccacgac cctgcaacaa cacctgatcg atggtcgtga
      301 catgattatc attcgcatgc cgaaagattt tccgccgttc ccgcagaaac tgaaattccg
      361 tgaaccgcaa cgtgaagaac gcatttgcct ggtcacgacc aactttcaga ccaaatcaat
      421 gagctctatg gttagcgata cgtcttgtac ctttccgagt tccgacggca tcttctggaa
      481 acattggatt cagaccaaag atggtcaatg cggcagtccg ctggtttcca cccgtgacgg
      541 tttcatcgtc ggcattcact cagcgtcgaa ctttacgaat accaacaatt acttcacgtc
      601 cgttccgaaa aactttatgg aactgctgac caatcaggaa gcgcagcaat gggtgtcagg
      661 ttggcgcctg aatgccgatt cggttctgtg gggcggtcac aaagtcttta tggtgaaacc
      721 ggaagaaccg ttccagccgg tcaaagaagc aacccaactg atgaatgaac tggtctactc
      781 ccaaaccggt taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata
//