BBa_K1319008
type: ComponentDefinition
persistent identity: BBa K1319008
display id: BBa_K1319008
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG-induced and T7-driven expression of TEV protease
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:50Z
mutable provenance: The part is combined from other BioBricks and a codon-optimized TEV protease CDS (K1319004).
owned by: james
owned by: myers
mutable description: This protein generator produces TEV protease when induced with IPTG in a DE3 strain.
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We created K1319004 in RFC[25] to enable potential
star: false
creator: Michael Osthege, Florian Gohr
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_I719005
component: BBa_K1319004
component: BBa_K1319106
component: BBa_B0034
component: BBa_G0002
component: BBa_B0015
component: BBa_K1319107
sequence annotation: BBa_I719005
sequence annotation: BBa_G0002
sequence annotation: BBa_B0034
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1319004
sequence annotation: BBa_K1319107
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1319008_sequence/1

BBa_I719005
type: Component
persistent identity: component2393319
display id: component2393319
version: 1
top level: BBa_K1319008
definition: pT7
access: public

BBa_K1319004
type: Component
persistent identity: component2393327
display id: component2393327
version: 1
top level: BBa_K1319008
definition: BBa_K1319004
access: public

BBa_K1319106
type: Component
persistent identity: component2393324
display id: component2393324
version: 1
top level: BBa_K1319008
definition: Scar25 ATG
access: public

BBa_B0034
type: Component
persistent identity: component2393322
display id: component2393322
version: 1
top level: BBa_K1319008
definition: BBa_B0034
access: public

BBa_G0002
type: Component
persistent identity: component2393320
display id: component2393320
version: 1
top level: BBa_K1319008
definition: SX scar
access: public

BBa_B0015
type: Component
persistent identity: component2393336
display id: component2393336
version: 1
top level: BBa_K1319008
definition: BBa_B0015
access: public

BBa_K1319107
type: Component
persistent identity: component2393329
display id: component2393329
version: 1
top level: BBa_K1319008
definition: Scar25 TAA
access: public

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2393319
display id: annotation2393319
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393319/range2393319/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393319/range2393319/1
type: Range
persistent identity: range2393319
display id: range2393319
version: 1
top level: BBa_K1319008
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2393320
display id: annotation2393320
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393320/range2393320/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393320/range2393320/1
type: Range
persistent identity: range2393320
display id: range2393320
version: 1
top level: BBa_K1319008
direction: direction/reverse
start: 24
end: 31
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393322
display id: annotation2393322
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393322/range2393322/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393322/range2393322/1
type: Range
persistent identity: range2393322
display id: range2393322
version: 1
top level: BBa_K1319008
direction: direction/reverse
start: 32
end: 43
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2393324
display id: annotation2393324
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393324/range2393324/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393324/range2393324/1
type: Range
persistent identity: range2393324
display id: range2393324
version: 1
top level: BBa_K1319008
direction: direction/reverse
start: 44
end: 58
orientation: inline

BBa_K1319004
type: SequenceAnnotation
persistent identity: annotation2393327
display id: annotation2393327
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393327/range2393327/1
component: BBa_K1319004

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393327/range2393327/1
type: Range
persistent identity: range2393327
display id: range2393327
version: 1
top level: BBa_K1319008
direction: direction/reverse
start: 59
end: 784
orientation: inline

BBa_K1319107
type: SequenceAnnotation
persistent identity: annotation2393329
display id: annotation2393329
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393329/range2393329/1
component: BBa_K1319107

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393329/range2393329/1
type: Range
persistent identity: range2393329
display id: range2393329
version: 1
top level: BBa_K1319008
direction: direction/reverse
start: 785
end: 801
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2393336
display id: annotation2393336
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393336/range2393336/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393336/range2393336/1
type: Range
persistent identity: range2393336
display id: range2393336
version: 1
top level: BBa_K1319008
direction: direction/reverse
start: 802
end: 930
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

pT7
type: ComponentDefinition
persistent identity: BBa I719005
display id: BBa_I719005
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Promoter
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:53Z
mutable provenance: ---
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Just a T7 Promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _128_
m user id: 0
owner id: 2097
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None
star: true
creator: Imperial 2007
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_I719005_sequence/1

SX scar
type: ComponentDefinition
persistent identity: BBa G0002
display id: BBa_G0002
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: XbaI and SpeI sites
owned by: james
owned by: myers
mutable description: XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0002_sequence/1

BBa_K1319004
type: ComponentDefinition
persistent identity: BBa K1319004
display id: BBa_K1319004
version: 1
was generated by: iGEM to SBOL conversion
description: TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:50Z
mutable provenance: The part was codon optimized using the genomic amino acid sequence, designed for RFC25 and mutated S219V for anti-self cleavage properties.
owned by: james
owned by: myers
mutable description: This part is a TEV protease in RFC25 that was optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation.
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The part does not contain start or stop codons, because it was designed for RFC25 compability
star: false
creator: Florian Gohr, Michael Osthege
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TEV protease
sequence annotation: S219V
sequence: https://synbiohub.org/public/igem/BBa_K1319004_sequence/1

TEV protease
type: SequenceAnnotation
persistent identity: annotation2386539
display id: annotation2386539
version: 1
top level: BBa_K1319004
location: https://synbiohub.org/public/igem/BBa_K1319004/annotation2386539/range2386539/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1319004/annotation2386539/range2386539/1
type: Range
persistent identity: range2386539
display id: range2386539
version: 1
top level: BBa_K1319004
direction: direction/forward
start: 1
end: 726
orientation: inline

S219V
type: SequenceAnnotation
persistent identity: annotation2386540
display id: annotation2386540
version: 1
top level: BBa_K1319004
location: https://synbiohub.org/public/igem/BBa_K1319004/annotation2386540/range2386540/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K1319004/annotation2386540/range2386540/1
type: Range
persistent identity: range2386540
display id: range2386540
version: 1
top level: BBa_K1319004
direction: direction/forward
start: 655
end: 657
orientation: inline

Scar25 ATG
type: ComponentDefinition
persistent identity: BBa K1319106
display id: BBa_K1319106
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Translation Start Scar Sequence
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: This sequence is produced by an assembly method.
owned by: james
owned by: myers
mutable description: This sequence is produced by RFC10-assembly of a RFC10-RBS with a RFC25-prefixed part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The resulting sequence is a combination of the RFC10 RBS-CDS scar (tactag) and 9 nucleotides from the RFC25 prefix (atg gcc ggc).
star: false
creator: Michael Osthege
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1319106_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2393317
display id: annotation2393317
version: 1
top level: Scar25 ATG
location: https://synbiohub.org/public/igem/BBa_K1319106/annotation2393317/range2393317/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1319106/annotation2393317/range2393317/1
type: Range
persistent identity: range2393317
display id: range2393317
version: 1
top level: Scar25 ATG
direction: direction/forward
start: 7
end: 9
orientation: inline

Scar25 TAA
type: ComponentDefinition
persistent identity: BBa K1319107
display id: BBa_K1319107
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Translation Stop Scar Sequence
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: This sequence is produced by an assembly method.
owned by: james
owned by: myers
mutable description: This sequence is produced by RFC10-assembly of a RFC25-CDS with a RFC10-prefixed part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The resulting sequence is a combination of 9 nucleotides from the RFC25 prefix (acc ggt taa) and the RFC10 scar (tactagag).
star: false
creator: Michael Osthege
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1319107_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2393318
display id: annotation2393318
version: 1
top level: Scar25 TAA
location: https://synbiohub.org/public/igem/BBa_K1319107/annotation2393318/range2393318/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1319107/annotation2393318/range2393318/1
type: Range
persistent identity: range2393318
display id: range2393318
version: 1
top level: Scar25 TAA
direction: direction/forward
start: 7
end: 9
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1319008_sequence/1
type: Sequence
persistent identity: BBa K1319008 sequence
display id: BBa_K1319008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggccggcggcgaaagcctgttcaaaggtccgcgtgactacaacccgattagctcgacgatctgccacctgacgaacgaaagcgacggccacaccacgagcctgtatggcatcggttttggcccgttcattatcacgaacaaacacctgtttcgtcgcaacaatggtaccctgctggtgcagtctctgcatggcgtgtttaaagttaaaaataccacgaccctgcaacaacacctgatcgatggtcgtgacatgattatcattcgcatgccgaaagattttccgccgttcccgcagaaactgaaattccgtgaaccgcaacgtgaagaacgcatttgcctggtcacgaccaactttcagaccaaatcaatgagctctatggttagcgatacgtcttgtacctttccgagttccgacggcatcttctggaaacattggattcagaccaaagatggtcaatgcggcagtccgctggtttccacccgtgacggtttcatcgtcggcattcactcagcgtcgaactttacgaataccaacaattacttcacgtccgttccgaaaaactttatggaactgctgaccaatcaggaagcgcagcaatgggtgtcaggttggcgcctgaatgccgattcggttctgtggggcggtcacaaagtctttatggtgaaaccggaagaaccgttccagccggtcaaagaagcaacccaactgatgaatgaactggtctactcccaaaccggttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I719005_sequence/1
type: Sequence
persistent identity: BBa I719005 sequence
display id: BBa_I719005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0002_sequence/1
type: Sequence
persistent identity: BBa G0002 sequence
display id: BBa_G0002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1319004_sequence/1
type: Sequence
persistent identity: BBa K1319004 sequence
display id: BBa_K1319004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcgaaagcctgttcaaaggtccgcgtgactacaacccgattagctcgacgatctgccacctgacgaacgaaagcgacggccacaccacgagcctgtatggcatcggttttggcccgttcattatcacgaacaaacacctgtttcgtcgcaacaatggtaccctgctggtgcagtctctgcatggcgtgtttaaagttaaaaataccacgaccctgcaacaacacctgatcgatggtcgtgacatgattatcattcgcatgccgaaagattttccgccgttcccgcagaaactgaaattccgtgaaccgcaacgtgaagaacgcatttgcctggtcacgaccaactttcagaccaaatcaatgagctctatggttagcgatacgtcttgtacctttccgagttccgacggcatcttctggaaacattggattcagaccaaagatggtcaatgcggcagtccgctggtttccacccgtgacggtttcatcgtcggcattcactcagcgtcgaactttacgaataccaacaattacttcacgtccgttccgaaaaactttatggaactgctgaccaatcaggaagcgcagcaatgggtgtcaggttggcgcctgaatgccgattcggttctgtggggcggtcacaaagtctttatggtgaaaccggaagaaccgttccagccggtcaaagaagcaacccaactgatgaatgaactggtctactcccaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1319106_sequence/1
type: Sequence
persistent identity: BBa K1319106 sequence
display id: BBa_K1319106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagatggccggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1319107_sequence/1
type: Sequence
persistent identity: BBa K1319107 sequence
display id: BBa_K1319107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggttaatactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z