BBa_K1319009 1 BBa_K1319009 mRFP-gal3-his fusion protein CDS 2014-10-01T11:00:00Z 2015-05-08T01:09:50Z it's a combination of other part sequences. This is a fusion protein created from E1010, K1319003 and K1319007. false false _1694_ 0 19522 9 Not in stock false The stop codon and barcode sequence was removed from E1010. We assembled the DNA by Gibson assembly, but designed the sequence to contain RFC[25] scars (ACCGGC) between the domains. false Michael Osthege annotation2392204 1 galectin-3 range2392204 1 771 1428 annotation2392201 1 RFC25 scar range2392201 1 765 770 annotation2392205 1 6xHis range2392205 1 1435 1452 annotation2392200 1 stop range2392200 1 1453 1458 annotation2392203 1 mRFP range2392203 1 1 764 annotation2392202 1 RFC25 scar range2392202 1 1429 1434 annotation2392199 1 start range2392199 1 1 3 BBa_K1319009_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctaccggcgcggacaacttctcactgcatgatgcgctgtctggcagcggcaatcccaatccccaggggtggccgggcgcgtggggtaaccaaccggccggagcaggcggttaccctggagctagctatccgggtgcctatccggggcaagcacctccgggcgcctatccgggacaggctccgcctggcgcatatccgggcgcaccgggtgcttacccgggtgcgccagcaccgggcgtttaccctggaccaccgtcaggtccaggtgcttatcccagtagtgggcaaccatcggccactggggcgtatccagcaacaggcccatatggtgcgcctgcgggtcctctgattgttccgtacaacttgccgttaccaggcggcgtagtgccgcgtatgctgatcaccattctgggtacggtgaaaccgaatgcgaatcgcattgcgctggattttcagcgtggcaatgacgttgcctttcacttcaatccgcgctttaacgagaacaatcgccgcgttattgtctgcaatacgaagcttgacaacaactggggtcgtgaagaacgccagtccgtgtttccctttgaaagcgggaaaccgttcaaaatccaggtgttggtggaaccggatcacttcaaagtcgccgtaaacgatgcgcatctgctccagtacaaccatcgggtcaagaaactcaacgagatttccaaattaggcatttcgggcgatatcgatctgacctctgccagctacaccatgatcaccggccaccaccaccaccaccactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z