LOCUS       BBa_K1319010             928 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutive expression of K1319000
ACCESSION   BBa_K1319010
VERSION     BBa_K1319010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    36..43
                     /label=BBa_G0002
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    63..65
                     /label=start
     misc_feature    57..71
                     /label=BBa_K1319106
     CDS             72..782
                     /label=EYFP
     misc_feature    72..782
                     /label=BBa_K1319000
     misc_feature    789..791
                     /label=stop
     misc_feature    783..799
                     /label=BBa_K1319107
     stem_loop       811..854
                     /label=stem_loop
     misc_feature    800..879
                     /label=BBa_B0010
     terminator      800..879
                     /label=BBa_B0010
     stem_loop       895..914
                     /label=T7 TE
     misc_feature    921..921
                     /label=stop
     polyA_site      915..928
                     /label=polya
     misc_feature    888..928
                     /label=BBa_B0012
     terminator      888..928
                     /label=BBa_B0012
     terminator      800..928
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatggccgg cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      121 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      181 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      241 ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc
      301 acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      361 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      421 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      481 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      541 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      601 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      661 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      721 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      781 acaccggtta atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      841 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      901 ccttcgggtg ggcctttctg cgtttata
//