The part was created from an I20260 part.
This part expresses K1319000 behind a J23101 constitutive promotor.
We designed the part by exchanging the ORF of E0040 in I20260 with the ORF of K1319000
RBS.3 (medium) -- derivative of BBa_0030
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Double terminator consisting of BBa_B0010 and BBa_B0012
XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry.
The part is an improved version of BBa_E0030
This part is a RFC[25]-compatible version of BBa_E0030. The start and stop codons have been removed to make it RFC[25]-compatible and the part is flanked by the RFC[25] prefix- and suffix-sequences.
start and stop-codons have been removed as they are present in the RFC[25] prefix/suffix sequences.
This sequence is produced by an assembly method.
This sequence is produced by RFC10-assembly of a RFC10-RBS with a RFC25-prefixed part.
The resulting sequence is a combination of the RFC10 RBS-CDS scar (tactag) and 9 nucleotides from the RFC25 prefix (atg gcc ggc).
This sequence is produced by RFC10-assembly of a RFC25-CDS with a RFC10-prefixed part.
The resulting sequence is a combination of 9 nucleotides from the RFC25 prefix (acc ggt taa) and the RFC10 scar (tactagag).
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1