LOCUS       BBa_K1319015            1666 bp    DNA     linear       29-Nov-2024
DEFINITION  Constitutive expression of GFP-EYFP fusion protein
ACCESSION   BBa_K1319015
VERSION     BBa_K1319015.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    36..43
                     /label=BBa_G0002
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    57..62
                     /label=BBa_G0000
     misc_feature    63..776
                     /label=BBa_K1051003
     misc_feature    777..782
                     /label=Scar 25
     misc_feature    777..782
                     /label=BBa_B0105
     misc_feature    783..803
                     /label=BBa_J18918
     misc_feature    804..809
                     /label=Scar 25
     misc_feature    804..809
                     /label=BBa_B0105
     CDS             810..1520
                     /label=EYFP
     misc_feature    810..1520
                     /label=BBa_K1319000
     misc_feature    1527..1529
                     /label=stop
     misc_feature    1521..1537
                     /label=BBa_K1319107
     stem_loop       1549..1592
                     /label=stem_loop
     misc_feature    1538..1617
                     /label=BBa_B0010
     terminator      1538..1617
                     /label=BBa_B0010
     stem_loop       1633..1652
                     /label=T7 TE
     misc_feature    1659..1659
                     /label=stop
     polyA_site      1653..1666
                     /label=polya
     misc_feature    1626..1666
                     /label=BBa_B0012
     terminator      1626..1666
                     /label=BBa_B0012
     terminator      1538..1666
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      121 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      181 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      241 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      301 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      361 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      421 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      481 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      541 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      601 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      661 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
      721 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaaaccg
      781 gcgaaaacct gtattttcag ggcaccggcg tgagcaaggg cgaggagctg ttcaccgggg
      841 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      901 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      961 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct
     1021 tcgcccgcta ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag
     1081 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1141 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1201 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1261 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1321 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1381 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     1441 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1501 tcggcatgga cgagctgtac accggttaat actagagcca ggcatcaaat aaaacgaaag
     1561 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1621 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//