LOCUS BBa_K1319017 1477 bp DNA linear 14-Nov-2024 DEFINITION LasI induced iLOV ACCESSION BBa_K1319017 VERSION BBa_K1319017.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23101 misc_feature 57..62 /label=BBa_G0000 misc_feature 36..43 /label=BBa_G0002 CDS 63..785 /label=BBa_C0179 misc_feature 791..798 /label=BBa_G0002 RBS 44..56 /label=RBS-3\Weak RBS 50..53 /label=RBS misc_feature 44..56 /label=BBa_B0032 RBS 44..56 /label=BBa_B0032 misc_feature 57..62 /label=BBa_G0000 CDS 63..785 /label=BBa_C0179 misc_feature 791..798 /label=BBa_G0002 stem_loop 810..853 /label=stem_loop misc_feature 799..878 /label=BBa_B0010 terminator 799..878 /label=BBa_B0010 stem_loop 894..913 /label=T7 TE misc_feature 920..920 /label=stop polyA_site 914..927 /label=polya misc_feature 887..927 /label=BBa_B0012 terminator 887..927 /label=BBa_B0012 terminator 799..927 /label=BBa_B0015 misc_feature 928..935 /label=BBa_G0002 misc_feature 940..955 /label=Las box misc_feature 988..988 /label=+1 promoter 936..988 /label=BBa_J64010 stem_loop 810..853 /label=stem_loop misc_feature 799..878 /label=BBa_B0010 terminator 799..878 /label=BBa_B0010 stem_loop 894..913 /label=T7 TE misc_feature 920..920 /label=stop polyA_site 914..927 /label=polya misc_feature 887..927 /label=BBa_B0012 terminator 887..927 /label=BBa_B0012 terminator 799..927 /label=BBa_B0015 misc_feature 989..994 /label=BBa_G0000 misc_feature 928..935 /label=BBa_G0002 misc_feature 999..1002 /label=conserved RBS 995..1006 /label=BBa_B0034 misc_feature 940..955 /label=Las box misc_feature 988..988 /label=+1 promoter 936..988 /label=BBa_J64010 misc_feature 1007..1012 /label=BBa_G0000 misc_feature 989..994 /label=BBa_G0000 CDS 1013..1348 /label=iLOV Domain misc_feature 1013..1348 /label=BBa_K660004 misc_feature 999..1002 /label=conserved RBS 995..1006 /label=BBa_B0034 misc_feature 1007..1012 /label=BBa_G0000 CDS 1013..1348 /label=iLOV Domain misc_feature 1013..1348 /label=BBa_K660004 stem_loop 1360..1403 /label=stem_loop misc_feature 1349..1428 /label=BBa_B0010 terminator 1349..1428 /label=BBa_B0010 stem_loop 1444..1463 /label=T7 TE misc_feature 1470..1470 /label=stop polyA_site 1464..1477 /label=polya misc_feature 1437..1477 /label=BBa_B0012 terminator 1437..1477 /label=BBa_B0012 terminator 1349..1477 /label=BBa_B0015 stem_loop 1360..1403 /label=stem_loop misc_feature 1349..1428 /label=BBa_B0010 terminator 1349..1428 /label=BBa_B0010 stem_loop 1444..1463 /label=T7 TE misc_feature 1470..1470 /label=stop polyA_site 1464..1477 /label=polya misc_feature 1437..1477 /label=BBa_B0012 terminator 1437..1477 /label=BBa_B0012 terminator 1349..1477 /label=BBa_B0015 ORIGIN 1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact 61 agatggcctt ggttgacggt tttcttgagc tggaacgctc aagtggaaaa ttggagtgga 121 gcgccatcct ccagaagatg gcgagcgacc ttggattctc gaagatcctg ttcggcctgt 181 tgcctaagga cagccaggac tacgagaacg ccttcatcgt cggcaactac ccggccgcct 241 ggcgcgagca ttacgaccgg gctggctacg cgcgggtcga cccgacggtc agtcactgta 301 cccagagcgt actgccgatt ttctgggaac cgtccatcta ccagacgcga aagcagcacg 361 agttcttcga ggaagcctcg gccgccggcc tggtgtatgg gctgaccatg ccgctgcatg 421 gtgctcgcgg cgaactcggc gcgctgagcc tcagcgtgga agcggaaaac cgggccgagg 481 ccaaccgttt catagagtcg gtcctgccga ccctgtggat gctcaaggac tacgcactgc 541 aaagcggtgc cggactggcc ttcgaacatc cggtcagcaa accggtggtt ctgaccagcc 601 gggagaagga agtgttgcag tggtgcgcca tcggcaagac cagttgggag atatcggtta 661 tctgcaactg ctcggaagcc aatgtgaact tccatatggg aaatattcgg cggaagttcg 721 gtgtgacctc ccgccgcgta gcggccatta tggccgttaa tttgggtctt attactctct 781 aataaatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 841 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 901 cttcgggtgg gcctttctgc gtttatatac tagagaaatc tatctcattt gctagttata 961 aaattatgaa atttgcataa attcttcata ctagaaagag gagaaatact agatgattga 1021 aaaaaacttt gtgattaccg atccgcgtct gccggataac ccgattattt ttgcgagcga 1081 tggctttctg gaactgaccg aatatagccg tgaagaaatt ctgggccgta acgcgcgttt 1141 tttgcagggc ccggaaaccg atcaggcgac cgtgcagaaa attcgtgatg cgattcgtga 1201 tcagcgtgaa accaccgtgc agctgattaa ctataccaaa agcggcaaaa aattttggaa 1261 cctgctgcat ttgcagccgg tgcgtgatca gaaaggcgaa ttgcagtatt ttattggcgt 1321 gcagctggat ggcagcgatc atgtgtaacc aggcatcaaa taaaacgaaa ggctcagtcg 1381 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 1441 actggctcac cttcgggtgg gcctttctgc gtttata //