BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_G0002
1
SX scar
SpeI/XbaI mixed site
2007-02-26T12:00:00Z
2015-08-31T04:07:27Z
XbaI and SpeI sites
XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry.
false
true
_41_
0
126
70
Not in stock
false
None.
false
Reshma Shetty
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
BBa_J64010
1
BBa_J64010
lasI promoter
2007-01-15T12:00:00Z
2015-08-31T01:56:26Z
Genomic sequence of Pseudomonas aeruginosa
Promoter driving the lasI gene in Pseudomonas aeruginosa. <br> Activated by LasR+3OC12HSL and not RhlR+C4HSL (Seed et al., 1995) <br> ~4x stronger than the lasB promoter (BBa_R0079; Seed et al., 1995). <br> Switch point ~0.1nM 3OC12HSL (Seed et al., 1995)
false
false
_98_
0
88
98
Not in stock
false
Promoter sequence cropped to 4nt upstream of Las box according to example of Whiteley and Greenberg, J. Bac, 2001
false
Jeffrey J Tabor
annotation1910616
1
+1
range1910616
1
53
53
annotation1910615
1
Las box
range1910615
1
5
20
BBa_K660004
1
BBa_K660004
iLOV
2011-09-15T11:00:00Z
2015-05-08T01:13:03Z
iLOV is a version of LOV2 that has been altered through site directed mutagenesis and DNA shuffling.
Released HQ 2013
iLOV has the same function and uses as LOV2. As a reporter, it is advantageous over GFP derived fluorescent proteins due to its small size (useful if you are tagging proteins), ability to recover quickly from photobleaching and use in anoxic conditions.
false
false
_837_
0
9421
9
In stock
true
illegal restriction sites. The part was synthesized to remove them.
false
Glasgow Uni 2011
annotation2135615
1
iLOV Domain
range2135615
1
1
336
BBa_C0179
1
lasR
lasR activator from P. aeruginosa PAO1(no LVA)
2004-05-26T11:00:00Z
2015-08-31T04:07:24Z
Released HQ 2013
same as C0079 except no LVA tag
false
false
_11_1_
0
61
7
In stock
false
true
jcbraff
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K1319017
1
BBa_K1319017
LasI induced iLOV
2014-10-03T11:00:00Z
2015-05-08T01:09:51Z
The part is combined from other parts
This device produces iLOV (K660004) in response to a quorum sensing input.
false
false
_1694_
0
19522
9
It's complicated
false
There's another scar between C0179 and B0015 that should not affect the functionality.
false
Arne Zimmermann, Michael Osthege
component2393514
1
BBa_K660004
component2393502
1
BBa_J64010
component2393476
1
BBa_J23101
component2393481
1
BBa_G0002
component2393527
1
BBa_B0015
component2393532
1
BBa_B0015
component2393518
1
BBa_G0000
component2393515
1
BBa_B0034
component2393503
1
BBa_B0015
component2393497
1
BBa_G0002
component2393482
1
BBa_B0032
component2393486
1
BBa_G0000
component2393511
1
BBa_G0000
component2393487
1
BBa_C0179
component2393478
1
BBa_G0002
component2393508
1
BBa_B0034
component2393522
1
BBa_K660004
component2393505
1
BBa_G0002
component2393510
1
BBa_G0000
component2393509
1
BBa_J64010
component2393495
1
BBa_B0015
component2393504
1
BBa_G0000
component2393477
1
BBa_G0000
component2393479
1
BBa_C0179
component2393489
1
BBa_G0002
annotation2393477
1
BBa_G0000
range2393477
1
57
62
annotation2393511
1
BBa_G0000
range2393511
1
989
994
annotation2393486
1
BBa_G0000
range2393486
1
57
62
annotation2393476
1
BBa_J23101
range2393476
1
1
35
annotation2393487
1
BBa_C0179
range2393487
1
63
785
annotation2393503
1
BBa_B0015
range2393503
1
799
927
annotation2393505
1
BBa_G0002
range2393505
1
928
935
annotation2393532
1
BBa_B0015
range2393532
1
1349
1477
annotation2393478
1
BBa_G0002
range2393478
1
36
43
annotation2393497
1
BBa_G0002
range2393497
1
928
935
annotation2393502
1
BBa_J64010
range2393502
1
936
988
annotation2393489
1
BBa_G0002
range2393489
1
791
798
annotation2393515
1
BBa_B0034
range2393515
1
995
1006
annotation2393527
1
BBa_B0015
range2393527
1
1349
1477
annotation2393504
1
BBa_G0000
range2393504
1
989
994
annotation2393514
1
BBa_K660004
range2393514
1
1013
1348
annotation2393481
1
BBa_G0002
range2393481
1
791
798
annotation2393482
1
BBa_B0032
range2393482
1
44
56
annotation2393508
1
BBa_B0034
range2393508
1
995
1006
annotation2393518
1
BBa_G0000
range2393518
1
1007
1012
annotation2393509
1
BBa_J64010
range2393509
1
936
988
annotation2393510
1
BBa_G0000
range2393510
1
1007
1012
annotation2393522
1
BBa_K660004
range2393522
1
1013
1348
annotation2393495
1
BBa_B0015
range2393495
1
799
927
annotation2393479
1
BBa_C0179
range2393479
1
63
785
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_G0000
1
scar
SpeI/XbaI scar for RBS-CDS junctions
2007-07-22T11:00:00Z
2015-08-31T04:07:27Z
SpeI/XbaI scar
This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
false
true
_41_
0
126
162
Not in stock
false
This is a shorter scar to ensure proper spacing between the RBS and CDS.
false
Reshma Shetty
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_C0179_sequence
1
atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa
BBa_K1319017_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataaatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaatctatctcatttgctagttataaaattatgaaatttgcataaattcttcatactagaaagaggagaaatactagatgattgaaaaaaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatggctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaacgcgcgttttttgcagggcccggaaaccgatcaggcgaccgtgcagaaaattcgtgatgcgattcgtgatcagcgtgaaaccaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgctgcatttgcagccggtgcgtgatcagaaaggcgaattgcagtattttattggcgtgcagctggatggcagcgatcatgtgtaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K660004_sequence
1
atgattgaaaaaaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatggctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaacgcgcgttttttgcagggcccggaaaccgatcaggcgaccgtgcagaaaattcgtgatgcgattcgtgatcagcgtgaaaccaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgctgcatttgcagccggtgcgtgatcagaaaggcgaattgcagtattttattggcgtgcagctggatggcagcgatcatgtgtaa
BBa_G0000_sequence
1
tactag
BBa_B0032_sequence
1
tcacacaggaaag
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
BBa_G0002_sequence
1
tactagag
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J64010_sequence
1
aaatctatctcatttgctagttataaaattatgaaatttgcataaattcttca
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z