BBa_K1319017
type: ComponentDefinition
persistent identity: BBa K1319017
display id: BBa_K1319017
version: 1
was generated by: iGEM to SBOL conversion
description: LasI induced iLOV
created: 2014-10-03T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: The part is combined from other parts
owned by: james
owned by: myers
mutable description: This device produces iLOV (K660004) in response to a quorum sensing input.
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: There's another scar between C0179 and B0015 that should not affect the functionality.
star: false
creator: Arne Zimmermann, Michael Osthege
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J23101
component: BBa_B0034
component: BBa_G0002
component: BBa_G0000
component: BBa_B0015
component: BBa_J64010
component: BBa_K660004
component: BBa_C0179
component: BBa_B0032
sequence annotation: BBa_J23101
sequence annotation: BBa_G0002
sequence annotation: BBa_B0032
sequence annotation: BBa_G0000
sequence annotation: BBa_C0179
sequence annotation: BBa_B0015
sequence annotation: BBa_J64010
sequence annotation: BBa_B0034
sequence annotation: BBa_K660004
sequence: https://synbiohub.org/public/igem/BBa_K1319017_sequence/1

BBa_J23101
type: Component
persistent identity: component2393476
display id: component2393476
version: 1
top level: BBa_K1319017
definition: BBa_J23101
access: public

BBa_B0034
type: Component
persistent identity: component2393508
display id: component2393508
version: 1
top level: BBa_K1319017
definition: BBa_B0034
access: public

BBa_G0002
type: Component
persistent identity: component2393505
display id: component2393505
version: 1
top level: BBa_K1319017
definition: SX scar
access: public

BBa_G0000
type: Component
persistent identity: component2393504
display id: component2393504
version: 1
top level: BBa_K1319017
definition: scar
access: public

BBa_B0015
type: Component
persistent identity: component2393503
display id: component2393503
version: 1
top level: BBa_K1319017
definition: BBa_B0015
access: public

BBa_J64010
type: Component
persistent identity: component2393502
display id: component2393502
version: 1
top level: BBa_K1319017
definition: BBa_J64010
access: public

BBa_G0002
type: Component
persistent identity: component2393497
display id: component2393497
version: 1
top level: BBa_K1319017
definition: SX scar
access: public

BBa_B0015
type: Component
persistent identity: component2393495
display id: component2393495
version: 1
top level: BBa_K1319017
definition: BBa_B0015
access: public

BBa_G0002
type: Component
persistent identity: component2393489
display id: component2393489
version: 1
top level: BBa_K1319017
definition: SX scar
access: public

BBa_B0015
type: Component
persistent identity: component2393527
display id: component2393527
version: 1
top level: BBa_K1319017
definition: BBa_B0015
access: public

BBa_K660004
type: Component
persistent identity: component2393522
display id: component2393522
version: 1
top level: BBa_K1319017
definition: BBa_K660004
access: public

BBa_G0000
type: Component
persistent identity: component2393518
display id: component2393518
version: 1
top level: BBa_K1319017
definition: scar
access: public

BBa_B0034
type: Component
persistent identity: component2393515
display id: component2393515
version: 1
top level: BBa_K1319017
definition: BBa_B0034
access: public

BBa_K660004
type: Component
persistent identity: component2393514
display id: component2393514
version: 1
top level: BBa_K1319017
definition: BBa_K660004
access: public

BBa_G0000
type: Component
persistent identity: component2393511
display id: component2393511
version: 1
top level: BBa_K1319017
definition: scar
access: public

BBa_G0000
type: Component
persistent identity: component2393510
display id: component2393510
version: 1
top level: BBa_K1319017
definition: scar
access: public

BBa_J64010
type: Component
persistent identity: component2393509
display id: component2393509
version: 1
top level: BBa_K1319017
definition: BBa_J64010
access: public

BBa_C0179
type: Component
persistent identity: component2393487
display id: component2393487
version: 1
top level: BBa_K1319017
definition: lasR
access: public

BBa_B0015
type: Component
persistent identity: component2393532
display id: component2393532
version: 1
top level: BBa_K1319017
definition: BBa_B0015
access: public

BBa_G0000
type: Component
persistent identity: component2393486
display id: component2393486
version: 1
top level: BBa_K1319017
definition: scar
access: public

BBa_B0032
type: Component
persistent identity: component2393482
display id: component2393482
version: 1
top level: BBa_K1319017
definition: BBa_B0032
access: public

BBa_G0002
type: Component
persistent identity: component2393481
display id: component2393481
version: 1
top level: BBa_K1319017
definition: SX scar
access: public

BBa_C0179
type: Component
persistent identity: component2393479
display id: component2393479
version: 1
top level: BBa_K1319017
definition: lasR
access: public

BBa_G0002
type: Component
persistent identity: component2393478
display id: component2393478
version: 1
top level: BBa_K1319017
definition: SX scar
access: public

BBa_G0000
type: Component
persistent identity: component2393477
display id: component2393477
version: 1
top level: BBa_K1319017
definition: scar
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2393476
display id: annotation2393476
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393476/range2393476/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393476/range2393476/1
type: Range
persistent identity: range2393476
display id: range2393476
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2393478
display id: annotation2393478
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393478/range2393478/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393478/range2393478/1
type: Range
persistent identity: range2393478
display id: range2393478
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 36
end: 43
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2393482
display id: annotation2393482
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393482/range2393482/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393482/range2393482/1
type: Range
persistent identity: range2393482
display id: range2393482
version: 1
top level: BBa_K1319017
direction: direction/forward
start: 44
end: 56
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2393486
display id: annotation2393486
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393486/range2393486/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393486/range2393486/1
type: Range
persistent identity: range2393486
display id: range2393486
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 57
end: 62
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2393477
display id: annotation2393477
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393477/range2393477/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393477/range2393477/1
type: Range
persistent identity: range2393477
display id: range2393477
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 57
end: 62
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2393487
display id: annotation2393487
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393487/range2393487/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393487/range2393487/1
type: Range
persistent identity: range2393487
display id: range2393487
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 63
end: 785
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2393479
display id: annotation2393479
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393479/range2393479/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393479/range2393479/1
type: Range
persistent identity: range2393479
display id: range2393479
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 63
end: 785
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2393489
display id: annotation2393489
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393489/range2393489/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393489/range2393489/1
type: Range
persistent identity: range2393489
display id: range2393489
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 791
end: 798
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2393481
display id: annotation2393481
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393481/range2393481/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393481/range2393481/1
type: Range
persistent identity: range2393481
display id: range2393481
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 791
end: 798
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2393503
display id: annotation2393503
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393503/range2393503/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393503/range2393503/1
type: Range
persistent identity: range2393503
display id: range2393503
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 799
end: 927
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2393495
display id: annotation2393495
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393495/range2393495/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393495/range2393495/1
type: Range
persistent identity: range2393495
display id: range2393495
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 799
end: 927
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2393497
display id: annotation2393497
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393497/range2393497/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393497/range2393497/1
type: Range
persistent identity: range2393497
display id: range2393497
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 928
end: 935
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2393505
display id: annotation2393505
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393505/range2393505/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393505/range2393505/1
type: Range
persistent identity: range2393505
display id: range2393505
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 928
end: 935
orientation: inline

BBa_J64010
type: SequenceAnnotation
persistent identity: annotation2393502
display id: annotation2393502
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393502/range2393502/1
component: BBa_J64010

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393502/range2393502/1
type: Range
persistent identity: range2393502
display id: range2393502
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 936
end: 988
orientation: inline

BBa_J64010
type: SequenceAnnotation
persistent identity: annotation2393509
display id: annotation2393509
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393509/range2393509/1
component: BBa_J64010

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393509/range2393509/1
type: Range
persistent identity: range2393509
display id: range2393509
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 936
end: 988
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2393504
display id: annotation2393504
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393504/range2393504/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393504/range2393504/1
type: Range
persistent identity: range2393504
display id: range2393504
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 989
end: 994
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2393511
display id: annotation2393511
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393511/range2393511/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393511/range2393511/1
type: Range
persistent identity: range2393511
display id: range2393511
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 989
end: 994
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393508
display id: annotation2393508
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393508/range2393508/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393508/range2393508/1
type: Range
persistent identity: range2393508
display id: range2393508
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 995
end: 1006
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393515
display id: annotation2393515
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393515/range2393515/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393515/range2393515/1
type: Range
persistent identity: range2393515
display id: range2393515
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 995
end: 1006
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2393518
display id: annotation2393518
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393518/range2393518/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393518/range2393518/1
type: Range
persistent identity: range2393518
display id: range2393518
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 1007
end: 1012
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2393510
display id: annotation2393510
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393510/range2393510/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393510/range2393510/1
type: Range
persistent identity: range2393510
display id: range2393510
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 1007
end: 1012
orientation: inline

BBa_K660004
type: SequenceAnnotation
persistent identity: annotation2393522
display id: annotation2393522
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393522/range2393522/1
component: BBa_K660004

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393522/range2393522/1
type: Range
persistent identity: range2393522
display id: range2393522
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 1013
end: 1348
orientation: inline

BBa_K660004
type: SequenceAnnotation
persistent identity: annotation2393514
display id: annotation2393514
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393514/range2393514/1
component: BBa_K660004

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393514/range2393514/1
type: Range
persistent identity: range2393514
display id: range2393514
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 1013
end: 1348
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2393527
display id: annotation2393527
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393527/range2393527/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393527/range2393527/1
type: Range
persistent identity: range2393527
display id: range2393527
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 1349
end: 1477
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2393532
display id: annotation2393532
version: 1
top level: BBa_K1319017
location: https://synbiohub.org/public/igem/BBa_K1319017/annotation2393532/range2393532/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1319017/annotation2393532/range2393532/1
type: Range
persistent identity: range2393532
display id: range2393532
version: 1
top level: BBa_K1319017
direction: direction/reverse
start: 1349
end: 1477
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

scar
type: ComponentDefinition
persistent identity: BBa G0000
display id: BBa_G0000
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI scar for RBS-CDS junctions
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: SpeI/XbaI scar
owned by: james
owned by: myers
mutable description: This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a shorter scar to ensure proper spacing between the RBS and CDS.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0000_sequence/1

SX scar
type: ComponentDefinition
persistent identity: BBa G0002
display id: BBa_G0002
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: XbaI and SpeI sites
owned by: james
owned by: myers
mutable description: XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0002_sequence/1

BBa_J64010
type: ComponentDefinition
persistent identity: BBa J64010
display id: BBa_J64010
version: 1
was generated by: iGEM to SBOL conversion
description: lasI promoter
created: 2007-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: Genomic sequence of Pseudomonas aeruginosa
owned by: james
owned by: myers
mutable description: Promoter driving the lasI gene in Pseudomonas aeruginosa. Activated by LasR+3OC12HSL and not RhlR+C4HSL (Seed et al., 1995) ~4x stronger than the lasB promoter (BBa_R0079; Seed et al., 1995). Switch point ~0.1nM 3OC12HSL (Seed et al., 1995)
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Promoter sequence cropped to 4nt upstream of Las box according to example of Whiteley and Greenberg, J. Bac, 2001
star: false
creator: Jeffrey J Tabor
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Las box
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_J64010_sequence/1

Las box
type: SequenceAnnotation
persistent identity: annotation1910615
display id: annotation1910615
version: 1
top level: BBa_J64010
location: https://synbiohub.org/public/igem/BBa_J64010/annotation1910615/range1910615/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_J64010/annotation1910615/range1910615/1
type: Range
persistent identity: range1910615
display id: range1910615
version: 1
top level: BBa_J64010
direction: direction/forward
start: 5
end: 20
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1910616
display id: annotation1910616
version: 1
top level: BBa_J64010
location: https://synbiohub.org/public/igem/BBa_J64010/annotation1910616/range1910616/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_J64010/annotation1910616/range1910616/1
type: Range
persistent identity: range1910616
display id: range1910616
version: 1
top level: BBa_J64010
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_K660004
type: ComponentDefinition
persistent identity: BBa K660004
display id: BBa_K660004
version: 1
was generated by: iGEM to SBOL conversion
description: iLOV
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:13:03Z
mutable provenance: iLOV is a version of LOV2 that has been altered through site directed mutagenesis and DNA shuffling.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: iLOV has the same function and uses as LOV2. As a reporter, it is advantageous over GFP derived fluorescent proteins due to its small size (useful if you are tagging proteins), ability to recover quickly from photobleaching and use in anoxic conditions.
discontinued: false
dominant: false
experience: experience/None
group u list: _837_
m user id: 0
owner id: 9421
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: illegal restriction sites. The part was synthesized to remove them.
star: false
creator: Glasgow Uni 2011
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: iLOV Domain
sequence: https://synbiohub.org/public/igem/BBa_K660004_sequence/1

iLOV Domain
type: SequenceAnnotation
persistent identity: annotation2135615
display id: annotation2135615
version: 1
top level: BBa_K660004
location: https://synbiohub.org/public/igem/BBa_K660004/annotation2135615/range2135615/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K660004/annotation2135615/range2135615/1
type: Range
persistent identity: range2135615
display id: range2135615
version: 1
top level: BBa_K660004
direction: direction/forward
start: 1
end: 336
orientation: inline

lasR
type: ComponentDefinition
persistent identity: BBa C0179
display id: BBa_C0179
version: 1
was generated by: iGEM to SBOL conversion
description: lasR activator from P. aeruginosa PAO1(no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0079 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0179_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1319017_sequence/1
type: Sequence
persistent identity: BBa K1319017 sequence
display id: BBa_K1319017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataaatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaatctatctcatttgctagttataaaattatgaaatttgcataaattcttcatactagaaagaggagaaatactagatgattgaaaaaaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatggctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaacgcgcgttttttgcagggcccggaaaccgatcaggcgaccgtgcagaaaattcgtgatgcgattcgtgatcagcgtgaaaccaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgctgcatttgcagccggtgcgtgatcagaaaggcgaattgcagtattttattggcgtgcagctggatggcagcgatcatgtgtaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0000_sequence/1
type: Sequence
persistent identity: BBa G0000 sequence
display id: BBa_G0000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0002_sequence/1
type: Sequence
persistent identity: BBa G0002 sequence
display id: BBa_G0002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64010_sequence/1
type: Sequence
persistent identity: BBa J64010 sequence
display id: BBa_J64010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaatctatctcatttgctagttataaaattatgaaatttgcataaattcttca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K660004_sequence/1
type: Sequence
persistent identity: BBa K660004 sequence
display id: BBa_K660004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgaaaaaaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatggctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaacgcgcgttttttgcagggcccggaaaccgatcaggcgaccgtgcagaaaattcgtgatgcgattcgtgatcagcgtgaaaccaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgctgcatttgcagccggtgcgtgatcagaaaggcgaattgcagtattttattggcgtgcagctggatggcagcgatcatgtgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0179_sequence/1
type: Sequence
persistent identity: BBa C0179 sequence
display id: BBa_C0179_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z