LOCUS BBa_K1319020 1613 bp DNA linear 29-Nov-2024 DEFINITION Translational unit of mRFP-galectin3-his ACCESSION BBa_K1319020 VERSION BBa_K1319020.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 19..21 /label=start misc_feature 1471..1476 /label=stop misc_feature 783..788 /label=RFC25 scar misc_feature 1447..1452 /label=RFC25 scar CDS 19..782 /label=mRFP CDS 789..1446 /label=galectin-3 misc_feature 1453..1470 /label=6xHis CDS 19..1476 /label=BBa_K1319009 stem_loop 1496..1539 /label=stem_loop misc_feature 1485..1564 /label=BBa_B0010 terminator 1485..1564 /label=BBa_B0010 stem_loop 1580..1599 /label=T7 TE misc_feature 1606..1606 /label=stop polyA_site 1600..1613 /label=polya misc_feature 1573..1613 /label=BBa_B0012 terminator 1573..1613 /label=BBa_B0012 terminator 1485..1613 /label=BBa_B0015 ORIGIN 1 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc 61 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa 121 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg 181 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa 241 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa 301 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa 361 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg 421 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac 481 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac 541 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa 601 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac 661 gaacgtgctg aaggtcgtca ctccaccggt gctaccggcg cggacaactt ctcactgcat 721 gatgcgctgt ctggcagcgg caatcccaat ccccaggggt ggccgggcgc gtggggtaac 781 caaccggccg gagcaggcgg ttaccctgga gctagctatc cgggtgccta tccggggcaa 841 gcacctccgg gcgcctatcc gggacaggct ccgcctggcg catatccggg cgcaccgggt 901 gcttacccgg gtgcgccagc accgggcgtt taccctggac caccgtcagg tccaggtgct 961 tatcccagta gtgggcaacc atcggccact ggggcgtatc cagcaacagg cccatatggt 1021 gcgcctgcgg gtcctctgat tgttccgtac aacttgccgt taccaggcgg cgtagtgccg 1081 cgtatgctga tcaccattct gggtacggtg aaaccgaatg cgaatcgcat tgcgctggat 1141 tttcagcgtg gcaatgacgt tgcctttcac ttcaatccgc gctttaacga gaacaatcgc 1201 cgcgttattg tctgcaatac gaagcttgac aacaactggg gtcgtgaaga acgccagtcc 1261 gtgtttccct ttgaaagcgg gaaaccgttc aaaatccagg tgttggtgga accggatcac 1321 ttcaaagtcg ccgtaaacga tgcgcatctg ctccagtaca accatcgggt caagaaactc 1381 aacgagattt ccaaattagg catttcgggc gatatcgatc tgacctctgc cagctacacc 1441 atgatcaccg gccaccacca ccaccaccac taataatact agagccaggc atcaaataaa 1501 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 1561 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //