BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1319009 1 BBa_K1319009 mRFP-gal3-his fusion protein CDS 2014-10-01T11:00:00Z 2015-05-08T01:09:50Z it's a combination of other part sequences. This is a fusion protein created from E1010, K1319003 and K1319007. false false _1694_ 0 19522 9 Not in stock false The stop codon and barcode sequence was removed from E1010. We assembled the DNA by Gibson assembly, but designed the sequence to contain RFC[25] scars (ACCGGC) between the domains. false Michael Osthege annotation2392199 1 start range2392199 1 1 3 annotation2392201 1 RFC25 scar range2392201 1 765 770 annotation2392203 1 mRFP range2392203 1 1 764 annotation2392204 1 galectin-3 range2392204 1 771 1428 annotation2392205 1 6xHis range2392205 1 1435 1452 annotation2392200 1 stop range2392200 1 1453 1458 annotation2392202 1 RFC25 scar range2392202 1 1429 1434 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1319020 1 BBa_K1319020 Translational unit of mRFP-galectin3-his 2014-09-30T11:00:00Z 2015-05-08T01:09:51Z The part is a combination of other BioBrick parts. This part is a translational unit of a mRFP-galectin-3-his (B0032.E1010.K1319003.K1319016.B0015) false false _1694_ 0 19522 9 In stock false The stop of K1319003 was removed and substituted by a His6-tag. false Michael Osthege component2392198 1 BBa_B0015 component2392190 1 BBa_B0034 component2392191 1 BBa_K1319009 annotation2392191 1 BBa_K1319009 range2392191 1 19 1476 annotation2392198 1 BBa_B0015 range2392198 1 1485 1613 annotation2392190 1 BBa_B0034 range2392190 1 1 12 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1319020_sequence 1 aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctaccggcgcggacaacttctcactgcatgatgcgctgtctggcagcggcaatcccaatccccaggggtggccgggcgcgtggggtaaccaaccggccggagcaggcggttaccctggagctagctatccgggtgcctatccggggcaagcacctccgggcgcctatccgggacaggctccgcctggcgcatatccgggcgcaccgggtgcttacccgggtgcgccagcaccgggcgtttaccctggaccaccgtcaggtccaggtgcttatcccagtagtgggcaaccatcggccactggggcgtatccagcaacaggcccatatggtgcgcctgcgggtcctctgattgttccgtacaacttgccgttaccaggcggcgtagtgccgcgtatgctgatcaccattctgggtacggtgaaaccgaatgcgaatcgcattgcgctggattttcagcgtggcaatgacgttgcctttcacttcaatccgcgctttaacgagaacaatcgccgcgttattgtctgcaatacgaagcttgacaacaactggggtcgtgaagaacgccagtccgtgtttccctttgaaagcgggaaaccgttcaaaatccaggtgttggtggaaccggatcacttcaaagtcgccgtaaacgatgcgcatctgctccagtacaaccatcgggtcaagaaactcaacgagatttccaaattaggcatttcgggcgatatcgatctgacctctgccagctacaccatgatcaccggccaccaccaccaccaccactaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1319009_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctaccggcgcggacaacttctcactgcatgatgcgctgtctggcagcggcaatcccaatccccaggggtggccgggcgcgtggggtaaccaaccggccggagcaggcggttaccctggagctagctatccgggtgcctatccggggcaagcacctccgggcgcctatccgggacaggctccgcctggcgcatatccgggcgcaccgggtgcttacccgggtgcgccagcaccgggcgtttaccctggaccaccgtcaggtccaggtgcttatcccagtagtgggcaaccatcggccactggggcgtatccagcaacaggcccatatggtgcgcctgcgggtcctctgattgttccgtacaacttgccgttaccaggcggcgtagtgccgcgtatgctgatcaccattctgggtacggtgaaaccgaatgcgaatcgcattgcgctggattttcagcgtggcaatgacgttgcctttcacttcaatccgcgctttaacgagaacaatcgccgcgttattgtctgcaatacgaagcttgacaacaactggggtcgtgaagaacgccagtccgtgtttccctttgaaagcgggaaaccgttcaaaatccaggtgttggtggaaccggatcacttcaaagtcgccgtaaacgatgcgcatctgctccagtacaaccatcgggtcaagaaactcaacgagatttccaaattaggcatttcgggcgatatcgatctgacctctgccagctacaccatgatcaccggccaccaccaccaccaccactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z