LOCUS       BBa_K1319042            1739 bp    DNA     linear       22-Dec-2024
DEFINITION  IPTG inducible iLOV
ACCESSION   BBa_K1319042
VERSION     BBa_K1319042.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=stop
     CDS             1..1084
                     /label=LacI
     misc_feature    1081..1084
                     /label=start
     RBS             1085..1092
                     /label=rbs
     promoter        1093..1153
                     /label=LacIq promoter
     misc_feature    1..1153
                     /label=BBa_K731300
     misc_feature    1194..1219
                     /label=lacO operator
     promoter        1162..1188
                     /label=Ptac promoter
     promoter        1..1239
                     /label=BBa_K731500
     RBS             1248..1260
                     /label=RBS-3\Weak
     RBS             1254..1257
                     /label=RBS
     misc_feature    1248..1260
                     /label=BBa_B0032
     RBS             1248..1260
                     /label=BBa_B0032
     CDS             1267..1602
                     /label=iLOV Domain
     misc_feature    1267..1602
                     /label=BBa_K660004
     stem_loop       1622..1665
                     /label=stem_loop
     misc_feature    1611..1690
                     /label=BBa_B0010
     terminator      1611..1690
                     /label=BBa_B0010
     stem_loop       1706..1725
                     /label=T7 TE
     misc_feature    1732..1732
                     /label=stop
     polyA_site      1726..1739
                     /label=polya
     misc_feature    1699..1739
                     /label=BBa_B0012
     terminator      1699..1739
                     /label=BBa_B0012
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
       61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
      301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
      901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
      961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt
     1201 gtgagcggat aacaattccc tcatgaaata attttgtttt actagagtca cacaggaaag
     1261 tactagatga ttgaaaaaaa ctttgtgatt accgatccgc gtctgccgga taacccgatt
     1321 atttttgcga gcgatggctt tctggaactg accgaatata gccgtgaaga aattctgggc
     1381 cgtaacgcgc gttttttgca gggcccggaa accgatcagg cgaccgtgca gaaaattcgt
     1441 gatgcgattc gtgatcagcg tgaaaccacc gtgcagctga ttaactatac caaaagcggc
     1501 aaaaaatttt ggaacctgct gcatttgcag ccggtgcgtg atcagaaagg cgaattgcag
     1561 tattttattg gcgtgcagct ggatggcagc gatcatgtgt aatactagag ccaggcatca
     1621 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1681 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//