BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K660004 1 BBa_K660004 iLOV 2011-09-15T11:00:00Z 2015-05-08T01:13:03Z iLOV is a version of LOV2 that has been altered through site directed mutagenesis and DNA shuffling. Released HQ 2013 iLOV has the same function and uses as LOV2. As a reporter, it is advantageous over GFP derived fluorescent proteins due to its small size (useful if you are tagging proteins), ability to recover quickly from photobleaching and use in anoxic conditions. false false _837_ 0 9421 9 In stock true illegal restriction sites. The part was synthesized to remove them. false Glasgow Uni 2011 annotation2135615 1 iLOV Domain range2135615 1 1 336 BBa_K731500 1 BBa_K731500 [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward 2012-08-21T11:00:00Z 2015-05-08T01:13:06Z pET21b This is E.coli lactose-inducibile promoter. lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription. Low, constitutive levels of transcription can be seen anyhow. false false _977_ 0 12142 9 In stock true false Francesco Guzzonato annotation2181542 1 rbs range2181542 1 1085 1092 annotation2181541 1 start range2181541 1 1081 1084 annotation2181546 1 Ptac promoter range2181546 1 1162 1188 annotation2181545 1 lacO operator range2181545 1 1194 1219 annotation2181540 1 LacI range2181540 1 1 1084 annotation2181543 1 LacIq promoter range2181543 1 1093 1153 annotation2181544 1 BBa_K731300 range2181544 1 1 1153 annotation2181539 1 stop range2181539 1 1 3 BBa_K1319042 1 BBa_K1319042 IPTG inducible iLOV 2014-09-29T11:00:00Z 2015-05-08T01:09:51Z The part was combined from previously existing BioBricks. This part can be used for IPTG-induced expression of K660004 (iLOV). false false _1694_ 0 19522 9 In stock false The part was assembled by Gibson assembly starting from K731520. The open reading frame was exchanged. false Ren?? Hanke component2390780 1 BBa_B0010 component2390774 1 BBa_K731500 component2390782 1 BBa_B0012 component2390776 1 BBa_B0032 component2390779 1 BBa_K660004 annotation2390780 1 BBa_B0010 range2390780 1 1611 1690 annotation2390779 1 BBa_K660004 range2390779 1 1267 1602 annotation2390774 1 BBa_K731500 range2390774 1 1 1239 annotation2390782 1 BBa_B0012 range2390782 1 1699 1739 annotation2390776 1 BBa_B0032 range2390776 1 1248 1260 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K731500_sequence 1 tcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattctactagaggttgacaattaatcatcggctcgtataatgtgtgggaattgtgagcggataacaattccctcatgaaataattttgttt BBa_K660004_sequence 1 atgattgaaaaaaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatggctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaacgcgcgttttttgcagggcccggaaaccgatcaggcgaccgtgcagaaaattcgtgatgcgattcgtgatcagcgtgaaaccaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgctgcatttgcagccggtgcgtgatcagaaaggcgaattgcagtattttattggcgtgcagctggatggcagcgatcatgtgtaa BBa_B0032_sequence 1 tcacacaggaaag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1319042_sequence 1 tcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattctactagaggttgacaattaatcatcggctcgtataatgtgtgggaattgtgagcggataacaattccctcatgaaataattttgttttactagagtcacacaggaaagtactagatgattgaaaaaaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatggctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaacgcgcgttttttgcagggcccggaaaccgatcaggcgaccgtgcagaaaattcgtgatgcgattcgtgatcagcgtgaaaccaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgctgcatttgcagccggtgcgtgatcagaaaggcgaattgcagtattttattggcgtgcagctggatggcagcgatcatgtgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z