LOCUS       BBa_K1320000            1413 bp    DNA     linear       23-May-2024
DEFINITION  Cd sensitive promoter with Ogr activator, PO promoter, and GFP
ACCESSION   BBa_K1320000
VERSION     BBa_K1320000.1  
FEATURES             Location/Qualifiers
     misc_feature    9..26
                     /label=ArsR binding site
     promoter        1..26
                     /label=BBa_K174016
     promoter        45..178
                     /label=cadA promoter
     misc_feature    52..57
                     /label=sigA -35
     misc_feature    76..81
                     /label=sigA -10
     misc_feature    90..120
                     /label=CzrA binding site
     promoter        35..205
                     /label=BBa_K174017
     misc_feature    214..225
                     /label=B0034
     misc_feature    232..232
                     /label=P2 ogr
     CDS             232..450
                     /label=P2 ogr
     misc_feature    214..450
                     /label=BBa_I746350
     promoter        459..550
                     /label=PO
     promoter        459..550
                     /label=BBa_I746361
     CDS             557..1276
                     /label=GFP protein
     CDS             557..1276
                     /label=BBa_E0040
     stem_loop       1296..1339
                     /label=stem_loop
     misc_feature    1285..1364
                     /label=BBa_B0010
     terminator      1285..1364
                     /label=BBa_B0010
     stem_loop       1380..1399
                     /label=T7 TE
     misc_feature    1406..1406
                     /label=stop
     polyA_site      1400..1413
                     /label=polya
     misc_feature    1373..1413
                     /label=BBa_B0012
     terminator      1373..1413
                     /label=BBa_B0012
ORIGIN
        1 agtaatcaaa ataaattgat ttattttact agaggcgctt gctgtttttc attgacactt
       61 tcttggaaaa caacatataa taggtgtaac ttatatatga gtatatgctc atatatataa
      121 aataaataca atactcattg atacgctttg aagagggaac cctaaatcgg aaggtaagct
      181 aagaggagga actactatgg ctagctacta gagaaagagg agaaatacta gatgtttcat
      241 tgtcctttat gccagcatgc cgcacatgcg cgtacaagtc gctatatcac tgacacgaca
      301 aaagagcgtt atcatcagtg ccagaacgtg aattgcagcg ccacgttcat cacttatgag
      361 tcggtacagc gatacatcgt gaagccggga gaagtccacg ccgtaaggcc gcacccgttg
      421 ccatcagggc agcaaattat gtggatgtaa tactagagcg cgccccgcga ttcgctaagg
      481 tgctgttgtg tcagtgataa gccatccggg actgatggcg gaggatgcgc atcgtcggga
      541 aactgatgcc tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
      601 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      661 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      721 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
      781 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      841 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
      901 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
      961 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     1021 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     1081 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     1141 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     1201 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     1261 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1321 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1381 gctcaccttc gggtgggcct ttctgcgttt ata
//