##gff-version 3
##sequence-region BBa_K132004 1 3127
BBa_K132004	.	terminator	2146	2186	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K132004	.	polyA_site	2173	2186	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132004	.	engineered_region	2146	2186	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132004	.	stem_loop	2153	2172	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132004	.	stop_codon	2179	2179	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132004	.	CDS	897	2024	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K132004	.	engineered_region	897	2024	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K132004	.	engineered_tag	2025	2049	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K132004	.	tag	1986	2024	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K132004	.	CDS	897	2024	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K132004	.	ribosome_entry_site	879	890	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K132004	.	conserved_region	883	886	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132004	.	engineered_region	2258	3127	.	+	0	ID=BBa_K132000;Name=BBa_K132000
BBa_K132004	.	engineered_region	2275	3088	.	+	0	ID=annotation1988217;Name=kanR gene;Parent=BBa_K132000
BBa_K132004	.	stop_codon	3122	3127	.	+	0	ID=annotation1988219;Name=stop;Parent=BBa_K132000
BBa_K132004	.	ribosome_entry_site	2258	2269	.	+	0	ID=annotation1987900;Name=RBS;Parent=BBa_K132000
BBa_K132004	.	sequence_feature	2270	2275	.	+	0	ID=annotation1988215;Name=scar;Parent=BBa_K132000
BBa_K132004	.	tag	3089	3121	.	+	0	ID=annotation1988218;Name=LVA;Parent=BBa_K132000
BBa_K132004	.	terminator	2058	2137	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K132004	.	stem_loop	2069	2112	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132004	.	engineered_region	2058	2137	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132004	.	engineered_region	1	870	.	+	0	ID=BBa_K132000;Name=BBa_K132000
BBa_K132004	.	engineered_region	18	831	.	+	0	ID=annotation1988217;Name=kanR gene;Parent=BBa_K132000
BBa_K132004	.	stop_codon	865	870	.	+	0	ID=annotation1988219;Name=stop;Parent=BBa_K132000
BBa_K132004	.	ribosome_entry_site	1	12	.	+	0	ID=annotation1987900;Name=RBS;Parent=BBa_K132000
BBa_K132004	.	sequence_feature	13	18	.	+	0	ID=annotation1988215;Name=scar;Parent=BBa_K132000
BBa_K132004	.	tag	832	864	.	+	0	ID=annotation1988218;Name=LVA;Parent=BBa_K132000
BBa_K132004	.	promoter	2195	2248	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K132004	.	promoter	2214	2219	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K132004	.	promoter	2237	2242	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K132004	.	engineered_region	2195	2248	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K132004	.	operator	2220	2236	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K132004	.	operator	2197	2213	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
>BBa_K132004
aaagaggagaaatactagatgagccatattcaacgggaaacgtcttgctccaggccgcgattaaattccaacatggatg
ctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcc
cgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaac
tggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactg
cgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagt
gttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcg
caatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtct
ggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttat
ttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatc
ctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgata
tgaataaattgcagtttcatttgatgctcgatgagtttttcgctgcaaacgacgaaaactacgctttagtagcttaata
atactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtg
tctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggc
gatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgcc
acctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcg
tggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcag
tgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgtta
tttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagc
atctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggc
tggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggt
tttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgg
gcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaaga
cagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttg
ctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctgg
cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactgga
aagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataaca
attgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagccatattcaac
gggaaacgtcttgctccaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataa
tgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaa
ggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatca
agcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattaga
agaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgt
aattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcga
gtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcacc
ggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgat
gttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcat
tacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatga
gtttttcgctgcaaacgacgaaaactacgctttagtagcttaataa