LOCUS       BBa_K132005             3127 bp    DNA     linear       22-Dec-2024
DEFINITION  kanR+lacI+Plac+kanR
ACCESSION   BBa_K132005
VERSION     BBa_K132005.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=RBS
     misc_feature    13..18
                     /label=scar
     misc_feature    18..831
                     /label=kanR gene
     misc_feature    832..864
                     /label=LVA
     misc_feature    865..870
                     /label=stop
     misc_feature    1..870
                     /label=BBa_K132000
     misc_feature    883..886
                     /label=conserved
     RBS             879..890
                     /label=BBa_B0034
     misc_feature    1986..2024
                     /label=LVA
     CDS             897..2024
                     /label=lacI-LVA
     misc_feature    897..2024
                     /label=BBa_C0012
     misc_feature    2025..2049
                     /label=Help:Barcodes
     CDS             897..2024
                     /label=BBa_C0012
     stem_loop       2069..2112
                     /label=stem_loop
     misc_feature    2058..2137
                     /label=BBa_B0010
     terminator      2058..2137
                     /label=BBa_B0010
     stem_loop       2153..2172
                     /label=T7 TE
     misc_feature    2179..2179
                     /label=stop
     polyA_site      2173..2186
                     /label=polya
     misc_feature    2146..2186
                     /label=BBa_B0012
     terminator      2146..2186
                     /label=BBa_B0012
     misc_feature    2197..2213
                     /label=lac O1
     promoter        2214..2219
                     /label=-35
     misc_feature    2220..2236
                     /label=lac O1
     promoter        2237..2242
                     /label=-10
     misc_feature    2195..2248
                     /label=BBa_R0011
     promoter        2195..2248
                     /label=BBa_R0011
     RBS             2258..2269
                     /label=RBS
     misc_feature    2270..2275
                     /label=scar
     misc_feature    2275..3088
                     /label=kanR gene
     misc_feature    3089..3121
                     /label=LVA
     misc_feature    3122..3127
                     /label=stop
     misc_feature    2258..3127
                     /label=BBa_K132000
ORIGIN
        1 aaagaggaga aatactagat gagccatatt caacgggaaa cgtcttgctc caggccgcga
       61 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      121 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg
      181 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
      241 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
      301 tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct
      361 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
      421 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
      481 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
      541 gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc
      601 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
      661 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
      721 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
      781 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt cgctgcaaac
      841 gacgaaaact acgctttagt agcttaataa tactagagaa agaggagaaa tactagatgg
      901 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
      961 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
     1021 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
     1081 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
     1141 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
     1201 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
     1261 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
     1321 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
     1381 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
     1441 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
     1501 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
     1561 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
     1621 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
     1681 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
     1741 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     1801 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     1861 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     1921 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     1981 ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt
     2041 gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2101 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2161 ttcgggtggg cctttctgcg tttatatact agagaattgt gagcggataa caattgacat
     2221 tgtgagcgga taacaagata ctgagcacat actagagaaa gaggagaaat actagatgag
     2281 ccatattcaa cgggaaacgt cttgctccag gccgcgatta aattccaaca tggatgctga
     2341 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     2401 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     2461 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     2521 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     2581 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     2641 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     2701 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     2761 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     2821 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     2881 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     2941 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     3001 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     3061 gtttcatttg atgctcgatg agtttttcgc tgcaaacgac gaaaactacg ctttagtagc
     3121 ttaataa
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